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Imaging proteins inside cells with fluorescent tags
Open accessAbstract
Watching biological molecules provides clues to their function and regulation. Some of the most powerful methods of labeling proteins for imaging use genetically encoded fluorescent fusion tags. There are four standard genetic methods of covalently tagging a protein with a fluorescent probe for cellular imaging. These use I) auto-fluorescent proteins, II) self-labeling enzymes, III) enzymes that catalyze the attachment of a probe to a target sequence, and IV) biarsenical dyes that target tetracysteine motifs. Each of these techniques has advantages and disadvantages. In this review, we cover new developments in these methods and discuss practical considerations for their use in imaging proteins inside living cells.
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Metadata
- Authors
- Crivat, G., & Taraska, J. W.
- Venue
- Trends in Biotechnology
- Publisher
- Elsevier BV
- Published
- 2012-01
- Volume
- 30
- Issue
- 1
- Pages
- 8-16
- ISSN
- 0167-7799 (ISSN-L)
- OpenAlex
- W1985283191
- Cited by
- 291
- References
- 77
- FWCI
- 1.48
- Type
- journal article
- Language
- en
- Open access
- green
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