Cited by 53Bacteriophages and microbial interactionsGut microbiota and healthCRISPR and Genetic Engineering

New technologies for developing phage-based tools to manipulate the human microbiome

Open access
Khan Mirzaei, Mohammadali, Deng, Li·2022·Trends in Microbiology

Abstract

Gut bacteria play an essential role in the human body by regulating multiple functions, producing essential metabolites, protecting against pathogen invasion, and much more. Conversely, changes in their community structure are linked to several gastrointestinal (GI) and non-GI conditions. Fortunately, these bacteria are amenable to external perturbations, but we need specific tools for their safe manipulation as nonspecific changes can cause unpredicted long-term consequences. Here, we mainly discuss recent advances in cultivation-independent technologies and argue their relevance to different key steps, that is, identifying the modulation targets and developing phage-based tools to precisely modulate gut bacteria and restore a sustainable microbiome in humans. We finally suggest multiple modulating strategies for different dysbiosis-associated diseases.

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Crossref · 10.1016/j.tim.2021.04.007

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  • Phages can eliminate cancer-associated bacteria.

    “Phages can eliminate cancer-associated bacteria, remodel the tumor microenvironment, enhance antitumor immunity, and deliver targeted therapeutic payloads.”

    From Phage therapy in oncology: opportunities for cancer prevention and treatment (2026) · cites this paper as [63]

    The “Phages as microbiota modulators” section (page unavailable) describes virulent phages as tools for eliminating unwanted bacteria. The section on disease-associated gut bacteria identifies *Helicobacter pylori*-associated gastric cancer, but the paper does not say that phages eliminate cancer-associated bacteria specifically. The claim applies a general proposed capability to a cancer setting that the cited paper does not establish. (LLM, conf=0.85)

    Experimental — accuracy not yet measured
  • Phages enable highly selective removal of cancer-associated bacteria within the gut or tumor niche, avoiding broad collateral damage to commensal communities associated with conventional antibiotics.

    “In parallel, phages enable highly selective removal of cancer-associated bacteria within the gut or tumor niche (Table 1), avoiding the broad collateral damage to commensal communities associated with conventional antibiotics [15,17,20,62,63]”

    From Phage therapy in oncology: opportunities for cancer prevention and treatment (2026) · cites this paper as [63]

    The “Phages as microbiota modulators” section says phages can target unwanted gut bacteria with high specificity while leaving nonhost bacteria unharmed. Figure 3 includes colorectal cancer among diseases for which phage-based microbiome modulation is a proposed goal. Our copy does not establish selective removal of cancer-associated bacteria in the gut or a tumor niche, or directly compare collateral damage with conventional antibiotics. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

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Authors
Khan Mirzaei, M., & Deng, L.
Affiliations
Helmholtz Munich (DE)
Corresponding author
Deng, Li
Venue
Trends in Microbiology
Publisher
Elsevier BV
Published
2022-02
Volume
30
Issue
2
Pages
131-142
ISSN
0966-842X (ISSN-L)
OpenAlex
W3163713499
Cited by
53
References
80
Citations by year
2026: 5 · 2025: 13 · 2024: 15 · 2023: 10 · 2022: 8 · 2021: 2
FWCI
0.98
Type
journal article
Language
en
Open access
Locations
  • Trends in Microbiology (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PuSH - Publication Server of Helmholtz Zentrum München (submittedVersion, cc-by-nc-nd)
  • mediaTUM – the media and publications repository of the Technical University Munich (Technical University Munich) (submittedVersion)
  • mediaTUM (Technical University of Munich) (submittedVersion, other-oa)
  • Site cant be reached (submittedVersion, cc-by-nc-nd)
Funders
  • European Research Council (ERC StG 803077)
  • Deutsche Forschungsgemeinschaft (273124240, 395357507)
  • Technical University of Munich (SFB 1371)
  • Helmholtz Zentrum München Deutsches Forschungszentrum für Umwelt und Gesundheit
Topics
  • Physical Sciences › Environmental Science › Ecology › Bacteriophages and microbial interactions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
Keywords
bacteriophages, human gut microbiome, microbiota modulation, dysbiosis, gut microbiota, microbiota restoration
MeSH terms
Gastrointestinal Microbiome, Bacteria, Bacteriophages, Humans, Microbiota, Dysbiosis

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