An experimental hut evaluation of PermaNet® 3.0, a deltamethrin–piperonyl butoxide combination net, against pyrethroid-resistant Anopheles gambiae and Culex quinquefasciatus mosquitoes in southern Benin
Abstract
PermaNet 3.0 is a long-lasting combination net with deltamethrin present on the sides and a mixture of deltamethrin and piperonyl butoxide (PBO), an oxidase synergist, on the top panel. An experimental hut trial comparing unwashed and 20 times washed PermaNet 3.0 and PermaNet 2.0, Olyset Net and a conventional deltamethrin-treated net washed three times was conducted in southern Benin. Anopheles gambiae and Culex quinquefasciatus from this area are highly resistant to pyrethroids through kdr and cytochrome P450 mechanisms. The unwashed PermaNet 3.0 killed slightly more A. gambiae (52%) than the unwashed PermaNet 2.0 (44%) (P=0.036), indicating only partial synergism of resistance. After washing there was significant loss of activity to a similar level, with PermaNet 3.0 killing 31%, PermaNet 2.0 killing 29% and the conventional net killing 26%. Blood-feeding rates were partially inhibited for unwashed PermaNet 3.0 and Olyset Net (27% inhibition). Personal protection against A. gambiae derived from PermaNet 3.0 was similar to that from PermaNet 2.0 before washing (50% vs. 47%), and after 20 washes it decreased to 30%. Against C. quinquefasciatus, no treatment killed >24% entering the huts. The synergism from unwashed PermaNet 3.0 was lower than expected, probably due to an unidentified resistance mechanism unaffected by PBO.
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New LLINs treated with a synergist such as piperonyl butoxide and pyrethroids insecticide have shown promising results against wild free-flying multi-resistant An. gambiae (s.s.) in experimental huts.
“Some of these have shown promising results against wild free-flying multi-resistant An. gambiae (s.s.) in experimental huts [34, 43–45].”
From Mapping insecticide resistance in Anopheles gambiae (s.l.) from Côte d'Ivoire (2018) · cites this paper as [45]
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All Nets are Equal, But Some Nets are More Equal Than Others
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- Authors
- N’Guessan, R., Asidi, A., Boko, P., Odjo, A., Akogbeto, M., Pigeon, O., & Rowland, M.
- Affiliations
- London School of Hygiene & Tropical Medicine (GB)
- Ministre de la Santé (BJ)
- Walloon Agricultural Research Centre (BE)
- Venue
- Transactions of the Royal Society of Tropical Medicine and Hygiene
- Publisher
- Oxford University Press (OUP)
- Published
- 2010-12
- Volume
- 104
- Issue
- 12
- Pages
- 758-765
- ISSN
- 0035-9203 (ISSN-L)
- OpenAlex
- W1969486521
- Cited by
- 101
- References
- 14
- Citations by year
- 2026: 5 · 2025: 2 · 2024: 7 · 2023: 9 · 2022: 5 · 2021: 10 · 2020: 12 · 2019: 2 · 2018: 5 · 2017: 7 · 2016: 4 · 2015: 4 · 2014: 4 · 2013: 14 · 2012: 6
- FWCI
- 7.97
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Transactions of the Royal Society of Tropical Medicine and Hygiene (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Keywords
- PermaNet 3.0, deltamethrin, piperonyl butoxide, insecticide-treated nets, pyrethroid resistance, Anopheles gambiae, Culex quinquefasciatus, kdr mutation, cytochrome P450, synergistic effect, net washing, blood-feeding inhibition, personal protection, malaria vector control, southern Benin
- MeSH terms
- Animals, Anopheles, Benin, Culex, Drug Resistance, Housing, Humans, Insecticides, Laundering, Malaria, Mosquito Control, Nitriles, Piperonyl Butoxide, Pyrethrins, Species Specificity, Insecticide-Treated Bednets
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