Dirofilaria immitis and Wolbachia-derived antigens: Its effect on endothelial mammal cells
Abstract
Antigens of both Dirofilaria immitis and Wolbachia symbiont bacteria are implicated in the inflammatory pathology of heartworm infection. The aim of the present study was to compare the stimulatory capacity of in vitro cultures of vascular endothelial cells by the adult somatic antigens of D. immitis (DiSA) and the recombinant form of the Wolbachia surface protein (rWSP), during the first 24h of stimulation. Our results indicate a different stimulatory activity of the two antigens. Both the DiSA and rWSP stimulate the production of the enzymes responsible of the arachidonic acid metabolism, cyclooxygenase-2, 5-lipoxygenase (5-LO), and leukotriene B4. Only DiSA stimulates the production of prostaglandin E2. Related to the adhesion molecules, the DiSA stimulates the expression of intercellular adhesion molecule-1 (ICAM-1) and platelet endothelial cell adhesion molecule-1 (PECAM-1), whereas rWSP stimulates ICAM-1, PECAM-1, and vascular cell adhesion molecule-1 (VCAM-1). Expression of E-cadherin and vascular endothelial growth factor also were stimulated by rWSP. Neither of the two antigens altered the basic physiological mechanisms of endothelial cells, such as cell proliferation, cell cycle, or apoptosis. The biological and pathological significance of these finding are discussed.
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Symbiotic bacteria stimulate proinflammatory eicosanoid production in vascular endothelium.
“which suggests that symbiotic bacteria stimulate proinflammatory eicosanoid production in the vascular endothelium.”
From Human and animal dirofilariasis: The emergence of a zoonotic mosaic (2012) · cites this paper as [394]
WSP-stimulated vascular endothelial cell cultures show increased expression of COX2, 5-LO, TxB2, and LTB4.
“Vascular endothelial cell cultures stimulated with WSP exhibit significant increases in the expression levels of enzymes responsible for eicosanoid synthesis (cyclooxygenase 2 [COX2] and 5-lipooxygenase [LO]) and of TxB2 and LTB4 (296, 394)”
From Human and animal dirofilariasis: The emergence of a zoonotic mosaic (2012) · cites this paper as [394]
Vascular endothelium is altered during D. immitis infection, undergoing inflammation and endothelial cell disorganization.
“the vascular endothelium is altered during D. immitis infection, undergoing inflammation and endothelial cell disorganization (394).”
From Human and animal dirofilariasis: The emergence of a zoonotic mosaic (2012) · cites this paper as [394]
Many molecules increased by Wolbachia antigens are also produced by endothelial cell cultures treated with DiSA.
“many of these molecules are also produced by endothelial cell cultures treated with DiSA (394)”
From Human and animal dirofilariasis: The emergence of a zoonotic mosaic (2012) · cites this paper as [394]
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Human and animal dirofilariasis: The emergence of a zoonotic mosaic
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- Authors
- Simón, F., Morchón, R., Rodríguez-Barbero, A., López-Belmonte, J., Grandi, G., & Genchi, C.
- Affiliations
- Universidad de Salamanca (ES)
- University of Milan (IT)
- Corresponding author
- Simón, F.
- Venue
- Veterinary Parasitology
- Publisher
- Elsevier BV
- Published
- 2008-12
- Volume
- 158
- Issue
- 3
- Pages
- 223-231
- ISSN
- 0304-4017 (ISSN-L)
- OpenAlex
- W2068200009
- Cited by
- 21
- References
- 31
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 2 · 2019: 1 · 2017: 1 · 2014: 1 · 2012: 2
- FWCI
- 1.93
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Veterinary Parasitology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
- Health Sciences › Veterinary › Small Animals › Helminth infection and control
- Life Sciences › Immunology and Microbiology › Parasitology › Parasites and Host Interactions
- Keywords
- Dirofilaria immitis, Wolbachia, heartworm infection, endothelial cells, vascular endothelial growth factor, arachidonic acid metabolism, cyclooxygenase-2, 5-lipoxygenase, leukotriene B4, prostaglandin E2, adhesion molecules, ICAM-1, CD31, VCAM-1, E-cadherin, Wolbachia surface protein, inflammatory pathology
- MeSH terms
- Animals, Antigens, Helminth, Cells, Cultured, Dirofilaria immitis, Endothelium, Vascular, Humans, Inflammation, Membrane Proteins, Intercellular Adhesion Molecule-1, Vascular Cell Adhesion Molecule-1, Platelet Endothelial Cell Adhesion Molecule-1, Wolbachia, Vascular Endothelial Growth Factor A, Endothelial Cells
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