iNOs expression is stimulated by the major surface protein (rWSP) from Wolbachia bacterial endosymbiont of Dirofilaria immitis following subcutaneous injection in mice
Abstract
The bacterial endosymbiont Wolbachia of several species of filarial nematodes plays an important role in the inflammatory pathology of filariasis. Nitric oxide (NO) production has also been implicated in the immune response during filarial infections. Here we present data indicating that a recombinant Wolbachia surface protein (rWSP) induces iNOs mRNA expression and NO production, as well as IFN-gamma and a Th1-type antibody response, in inoculated BALB/c mice. This effect is not observed when mice are inoculated with a recombinant heat shock protein from Wolbachia (GroEL).
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In amicrofilaremic canine D. immitis infections, a Th1 response with lack of IL-10 expression and elevated iNOS and IgG2 production occurs.
“a Th1 response characterized by a lack of IL-10 expression and elevated levels of expression of inducible nitric oxide (NO) synthase (iNOS) and IgG2 production occurs in amicrofilaremic canine infections (287).”
From Human and animal dirofilariasis: The emergence of a zoonotic mosaic (2012) · cites this paper as [287]
In microfilaremic canine D. immitis infections, a Th2 response with high IL-4 and IL-10 mRNAs and IgG1 production predominates.
“A Th2 response characterized by high levels of interleukin 4 (IL-4) and IL-10 mRNAs and IgG1 production predominates in microfilaremic canine infections.”
From Human and animal dirofilariasis: The emergence of a zoonotic mosaic (2012) · cites this paper as [287]
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- Authors
- Morchón, R., Bazzocchi, C., López-Belmonte, J., Martín-Pacho, J. R., Kramer, L. H., Grandi, G., & Simón, F.
- Affiliations
- Universidad de Salamanca (ES)
- University of Milan (IT)
- University of Parma (IT)
- Corresponding author
- Simón, F.
- Venue
- Parasitology International
- Publisher
- Elsevier BV
- Published
- 2007-03
- Volume
- 56
- Issue
- 1
- Pages
- 71-75
- ISSN
- 1383-5769 (ISSN-L)
- OpenAlex
- W2006735468
- Cited by
- 30
- References
- 19
- Citations by year
- 2026: 1 · 2024: 1 · 2022: 2 · 2021: 1 · 2020: 3 · 2017: 2 · 2014: 3 · 2013: 1 · 2012: 2
- FWCI
- 1.23
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Parasitology International (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
- Life Sciences › Immunology and Microbiology › Parasitology › Parasites and Host Interactions
- Life Sciences › Immunology and Microbiology › Immunology › Invertebrate Immune Response Mechanisms
- Keywords
- Wolbachia, Dirofilaria immitis, nitric oxide synthase, iNOS mRNA expression, nitric oxide production, filarial nematodes, filariasis, interferon-gamma, Th1 response, endosymbiotic bacteria, subcutaneous injection
- MeSH terms
- Animals, Antibodies, Bacterial, Bacterial Outer Membrane Proteins, Dirofilaria immitis, Dirofilariasis, Inflammation, Interferon-gamma, Mice, Inbred BALB C, Nitric Oxide, Recombinant Proteins, RNA, Messenger, Symbiosis, Interleukin-4, Interleukin-10, Th1 Cells, Chaperonin 60, Wolbachia, Mice, Nitric Oxide Synthase Type II
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