The apical/basal-polarity determinant Scribble cooperates with the PCP core factor Stbm/Vang and functions as one of its effectors
Open accessAbstract
Most tissues display several features of cellular polarization. Besides the ubiquitous epithelial polarization in the Apical-Basal (A/B) axis, many epithelia (and associated organs) display a Planar Cell Polarization (PCP). Recently, a crosstalk between the PCP and A/B polarity determinants has been suggested, i.e. the activity or stability of the PCP factor Frizzled is regulated by the A/B determinants aPKC and Bazooka in the Drosophila eye. We have systematically investigated genetic and physical interactions between the Drosophila A/B factors and the core PCP component Strabismus (Stbm)/Van Gogh (Vang). The A/B determinant Scribble was found to interact both genetically and physically with Stbm/Vang. We demonstrate that Scribble binds Stbm/Vang through its PDZ domain 3 and that it cooperates with Stbm/Vang in PCP establishment. Our data indicate that Scribble, in addition to its role in A/B polarity, has a distinct requirement in PCP establishment in the Drosophila eye and wing. We define a scribble allele that is largely PCP specific. Our data show that Scribble is part of the Stbm/Vang PCP complex and further suggest that it might act as an effector of Stbm/Vang during PCP establishment.
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A very similar mechanism links Drosophila Vang and Scribble in planar polarization of the wing hairs and photoreceptor cells.
“Intriguingly, a very similar mechanism appears to link Drosophila Vang and Scribble in planar polarization of the wing hairs and photoreceptor cells (Courbard et al., 2009).”
From Scribble: A master scaffold in polarity, adhesion, synaptogenesis, and proliferation (2019) · cites this paper as Courbard et al., 2009
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Scribble: A master scaffold in polarity, adhesion, synaptogenesis, and proliferation
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- Authors
- Courbard, J.-R., Djiane, A., Wu, J., & Mlodzik, M.
- Affiliations
- Icahn School of Medicine at Mount Sinai (US)
- Corresponding author
- Mlodzik, Marek
- Venue
- Developmental Biology
- Publisher
- Elsevier BV
- Published
- 2009-09
- Volume
- 333
- Issue
- 1
- Pages
- 67-77
- ISSN
- 0012-1606 (ISSN-L)
- PMC
- PMC3011816
- OpenAlex
- W2047339425
- Cited by
- 82
- References
- 46
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 2 · 2022: 4 · 2021: 6 · 2020: 6 · 2019: 1 · 2018: 4 · 2017: 6 · 2016: 9 · 2015: 4 · 2014: 3 · 2013: 4 · 2012: 10
- FWCI
- 4.16
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 1415 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Developmental Biology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Eye Institute (EY 13256, R01EY013256)
- National Institute of General Medical Sciences (R01GM062917, GM 62917)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Hippo pathway signaling and YAP/TAZ
- Keywords
- Scribble, strabismus, Vincent van Gogh, planar cell polarity, apical-basal polarity, PDZ domain, protein-protein interaction, Drosophila eye, Drosophila wing, epithelial polarization
- MeSH terms
- Animals, Drosophila, Membrane Proteins, Mutation, Protein Binding, Wings, Animal, Cell Polarity, Body Patterning, Drosophila Proteins, Compound Eye, Arthropod
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