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Cited by 53Axon Guidance and Neuronal SignalingNeurobiology and Insect Physiology ResearchDevelopmental Biology and Gene Regulation

Postembryonic lineages of the Drosophila brain: I. Development of the lineage-associated fiber tracts

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Lovick, Jennifer K., Ngo, Kathy T., Omoto, Jaison J., Wong, Darren C., Nguyen, Joseph D., Hartenstein, Volker·2013·Developmental Biology
DOI 10.1016/j.ydbio.2013.07.008PMC PMC3886848
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Abstract

Neurons of the Drosophila central brain fall into approximately 100 paired groups, termed lineages. Each lineage is derived from a single asymmetrically-dividing neuroblast. Embryonic neuroblasts produce 1,500 primary neurons (per hemisphere) that make up the larval CNS followed by a second mitotic period in the larva that generates approximately 10,000 secondary, adult-specific neurons. Clonal analyses based on previous works using lineage-specific Gal4 drivers have established that such lineages form highly invariant morphological units. All neurons of a lineage project as one or a few axon tracts (secondary axon tracts, SATs) with characteristic trajectories, thereby representing unique hallmarks. In the neuropil, SATs assemble into larger fiber bundles (fascicles) which interconnect different neuropil compartments. We have analyzed the SATs and fascicles formed by lineages during larval, pupal, and adult stages using antibodies against membrane molecules (Neurotactin/Neuroglian) and synaptic proteins (Bruchpilot/N-Cadherin). The use of these markers allows one to identify fiber bundles of the adult brain and associate them with SATs and fascicles of the larval brain. This work lays the foundation for assigning the lineage identity of GFP-labeled MARCM clones on the basis of their close association with specific SATs and neuropil fascicles, as described in the accompanying paper (Wong et al., 2013. Postembryonic lineages of the Drosophila brain: II. Identification of lineage projection patterns based on MARCM clones. Submitted.).

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Crossref · 10.1016/j.ydbio.2013.07.008

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118 of 118 references verified0 citation errors · 0 not checkable

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  • The majority of hemilineage tracts visible in the EM dataset occur in pairs or small groups (3-5).

    “the majority of hemilineage tracts visible in the EM dataset occur in pairs or small groups (3–5).”

    From Whole-brain annotation and multi-connectome cell typing of Drosophila (2024) · cites this paper as [31]

    The Discussion section, “Hemilineages and sub-lineages form separate tracts” (page unavailable), says that two hemilineage tracts from one lineage enter close together and remain close in the majority of lineages possessing two hemilineages. It does not establish that a majority of tracts visible in an EM dataset occur in pairs or groups of 3–5; the Methods describe confocal microscopy instead. (LLM, conf=0.83)

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  • From light-level data that labels a whole lineage and all of its hemilineages together, it is occasionally difficult to assess whether there is a single hemilineage or multiple if the cell body clusters do not separate sufficiently.

    “From light-level data that labels a whole lineage and all of its hemilineages together,139,199,200,201 it is occasionally difficult to assess whether there is a single hemilineage or multiple, if the cell body clusters happen to not separate sufficiently.”

    From Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster (2024) · cites this paper as [199]

  • Our lineage-associated tracts have been mapped by light microscopy and assigned to named lineage clones.

    “Our lineage-associated tracts have been mapped by light microscopy and assigned to named lineage clones.139,199,200,201,202”

    From Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster (2024) · cites this paper as [199]

  • The following lineages are new to the Hartenstein nomenclature: SLPal5/BLAd5, SLPav3/BLVa2a, LHl3/BLVa2b, SLPpl3/BLVa2c, PBp1/CM6, SLPpl2/CP6, SMPpd2/DPLc6, PSp1/DPMl2 and LHp3/CP5.

    “New to ref. 31 (Hartenstein nomenclature) are: SLPal5/BLAd5, SLPav3/BLVa2a, LHl3/BLVa2b, SLPpl3/BLVa2c, PBp1/CM6, SLPpl2/CP6, SMPpd2/DPLc6, PSp1/DPMl2 and LHp3/CP5”

    From Whole-brain annotation and multi-connectome cell typing of Drosophila (2024) · cites this paper as [31]

  • Previous literature expected a total of around 119 lineages in the central brain, including the gnathal ganglia (GNG).

    “From previous literature, we expected a total of around 119 lineages in the central brain, including the gnathal ganglia (GNG)31–34,84.”

    From Whole-brain annotation and multi-connectome cell typing of Drosophila (2024) · cites this paper as [31]

    The Introduction says previous studies mapped central-brain lineages, and the Results classify individual lineages, but neither passage gives an expected total of around 119 including the gnathal ganglia. Searches for “119” and for the gnathal or subesophageal ganglion did not find that estimate in the paper’s body text. The available passages have no page numbers. (LLM, conf=0.70)

    Experimental — accuracy not yet measured

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Cites

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3 of the 121 references couldn’t be matched to a known paper.

Research works printed but not linked: 4

  1. Hartenstein V Campos-Ortega JA 1984 Early neurogenesis in wild-type Drosophila melanogaster Roux’s Arch Dev Biol 193 308 325 28305340
  2. Kumar A Bello B Reichert H 2009b Lineage-specific cell death in postem-bryonic brain development of Drosophila Development 136 3433 3442 19762424
  3. Stocker RF Heimbeck G Gendre N de Belle JS 1997 Neuroblast ablation in Drosophila P[Gal4] lines reveals origins of olfactory interneurons J Neurobiol 32 443 456 9110257
  4. Strausfeld NJ Li Y 1999 Representation of the calyces in the medial and vertical lobes of cockroach mushroom bodies J Comp Neurol 409 626 646 10376744

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Authors
Lovick, J. K., Ngo, K. T., Omoto, J. J., Wong, D. C., Nguyen, J. D., & Hartenstein, V.
Affiliations
University of California, Los Angeles (US)
Corresponding author
Hartenstein, Volker
ORCID
Venue
Developmental Biology
Publisher
Elsevier BV
Published
2013-12
Volume
384
Issue
2
Pages
228-257
ISSN
0012-1606 (ISSN-L)
DOI
10.1016/j.ydbio.2013.07.008
PMC
PMC3886848
OpenAlex
W2008227188
Cited by
48
References
121
Citations by year
2026: 3 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 3 · 2020: 5 · 2019: 3 · 2018: 3 · 2017: 8 · 2016: 4 · 2015: 5 · 2014: 6 · 2013: 1
FWCI
2.15
Type
journal article
Language
en
Open access
Licence
Locations
  • Developmental Biology (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • NIH (R01 NS29357-15)
  • NSF Graduate Research Fellowship Program (DGE-0707424)
  • Ruth L. Kirschstein National Research Service Award (GM007185)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
Drosophila brain, neuronal lineage, fascicles, neuroblasts, adult brain, synaptic proteins, lineage identity
MeSH terms
Animals, Brain, Drosophila, Humans, Metamorphosis, Biological, Body Patterning

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