Clonal Development and Organization of the Adult Drosophila Central Brain
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Background The insect brain can be divided into neuropils that are formed by neurites of both local and remote origin. The complexity of the interconnections obscures how these neuropils are established and interconnected through development. The Drosophila central brain develops from a fixed number of neuroblasts (NBs) that deposit neurons in regional clusters. Results By determining individual NB clones and pursuing their projections into specific neuropils, we unravel the regional development of the brain neural network. Exhaustive clonal analysis revealed 95 stereotyped neuronal lineages with characteristic cell-body locations and neurite trajectories. Most clones show complex projection patterns, but despite the complexity, neighboring clones often coinnervate the same local neuropil or neuropils and further target a restricted set of distant neuropils. Conclusions These observations argue for regional clonal development of both neuropils and neuropil connectivity throughout the Drosophila central brain.
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Previous literature expected a total of around 119 lineages in the central brain, including the gnathal ganglia (GNG).
“From previous literature, we expected a total of around 119 lineages in the central brain, including the gnathal ganglia (GNG)31–34,84.”
From Whole-brain annotation and multi-connectome cell typing of Drosophila (2024) · cites this paper as [34]
The Introduction reports an earlier count of 106 neuroblasts in the procephalic region, and the Discussion reports 114 lineages across two collections, including some possibly derived from the optic lobe. Neither passage gives an expected total of around 119 lineages including the gnathal ganglia; the paper treats the subesophageal ganglion as distinct from its cerebral focus. Page numbers are unavailable in our copy. (LLM, conf=0.81)
Experimental — accuracy not yet measuredThe following lineages are new to the Ito/Lee nomenclature: CREl1/DALv3, LHp3/CP5, DILP/DILP, LALa1/BAlp2, SMPpm1/DPMm2 and VLPl5/BLVa3_or_4.
“new to refs. 33,34 (ItoLee nomenclature) are: CREl1/DALv3, LHp3/CP5, DILP/DILP, LALa1/BAlp2, SMPpm1/DPMm2 and VLPl5/BLVa3_or_4”
From Whole-brain annotation and multi-connectome cell typing of Drosophila (2024) · cites this paper as [34]
The Results section on regional clustering (page unavailable) lists LH lineages only through LHp2 and identifies the two CRE lineages as CREa1 and CREa2, supporting that LHp3 and CREl1 are new relative to this paper's nomenclature. Searches for the other specified names and paired identifiers found no matching passage, but the available passages do not establish each proposed lineage correspondence or its novelty. (LLM, conf=0.76)
Experimental — accuracy not yet measuredLight-level clones from refs. 33,34 match very well the great majority of the time.
“although light-level clones from refs. 33,34 match very well the great majority of the time”
From Whole-brain annotation and multi-connectome cell typing of Drosophila (2024) · cites this paper as [34]
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- Authors
- Yu, H.-H., Awasaki, T., Schroeder, M. D., Long, F., Yang, J. S., He, Y., Ding, P., Kao, J.-C., Wu, G. Y.-Y., Peng, H., Myers, G., & Lee, T.
- Affiliations
- Howard Hughes Medical Institute (US)
- Corresponding author
- Lee, Tzumin
- Venue
- Current Biology
- Publisher
- Elsevier BV
- Published
- 2013-04
- Volume
- 23
- Issue
- 8
- Pages
- 633-643
- ISSN
- 0960-9822 (ISSN-L)
- PMC
- PMC3637848
- OpenAlex
- W2123114798
- Cited by
- 163
- References
- 46
- Citations by year
- 2026: 7 · 2025: 10 · 2024: 9 · 2023: 19 · 2022: 9 · 2021: 10 · 2020: 18 · 2019: 12 · 2018: 6 · 2017: 16 · 2016: 6 · 2015: 14 · 2014: 18 · 2013: 9
- FWCI
- 7.69
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 386 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Current Biology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- Howard Hughes Medical Institute
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- neuronal lineage, neuronal migration, neural network organization, insect brain development
- MeSH terms
- Animals, Brain, Clone Cells, Drosophila melanogaster, Female, Larva, Male, Microscopy, Confocal, Cell Lineage, Neuropil, Neural Stem Cells
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