CoChat
Sign Up
Cited by 139Microbial Metabolic Engineering and BioproductionBiofuel production and bioconversionAnaerobic Digestion and Biogas Production

The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.

Acetone production with metabolically engineered strains of Acetobacterium woodii

Hoffmeister, Sabrina, Gerdom, Marzena, Bengelsdorf, Frank R., Linder, Sonja, Flüchter, Sebastian, Öztürk, Hatice, Blümke, Wilfried, May, Antje, Fischer, Ralf-Jörg, Bahl, Hubert, Dürre, Peter·2016·Metabolic Engineering
DOI 10.1016/j.ymben.2016.03.001
Open this paper in CoChat — free account

Abstract

Expected depletion of oil and fossil resources urges the development of new alternative routes for the production of bulk chemicals and fuels beyond petroleum resources. In this study, the clostridial acetone pathway was used for the formation of acetone in the acetogenic bacterium Acetobacterium woodii. The acetone production operon (APO) containing the genes thlA (encoding thiolase A), ctfA/ctfB (encoding CoA transferase), and adc (encoding acetoacetate decarboxylase) from Clostridium acetobutylicum were cloned under the control of the thlA promoter into four vectors having different replicons for Gram-positives (pIP404, pBP1, pCB102, and pCD6). Stable replication was observed for all constructs. A. woodii [pJIR_actthlA] achieved the maximal acetone concentration under autotrophic conditions (15.2±3.4mM). Promoter sequences of the genes ackA from A. woodii and pta-ack from C. ljungdahlii were determined by primer extension (PEX) and cloned upstream of the APO. The highest acetone production in recombinant A. woodii cells was achieved using the promoters PthlA and Ppta-ack. Batch fermentations using A. woodii [pMTL84151_actthlA] in a bioreactor revealed that acetate concentration had an effect on the acetone production, due to the high Km value of the CoA transferase. In order to establish consistent acetate concentration within the bioreactor and to increase biomass, a continuous fermentation process for A. woodii was developed. Thus, acetone productivity of the strain A. woodii [pMTL84151_actthlA] was increased from 1.2mgL(-1)h(-1) in bottle fermentation to 26.4mgL(-1)h(-1) in continuous gas fermentation.

Overview

Integrity signals

Registry

Crossref · 10.1016/j.ymben.2016.03.001

Cites

42 of 42 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 42 references

Research works printed but not linked: 2

  1. LanzaTech, 2012. LanzaTech and Baosteel׳s 100,000 gallon/year waste-gas-to-ethanol pre-commercial facility exceeds productivity expectations and hits major milestones in advance of commercialization (Press release). 〈http://www.lanzatech.com/lanzatech-and-baosteels-100000-gallonyear-waste-gas-to-ethanol-pre-commercial-facility-exceeds-productivity-expectations-and-hits-major-milestones-in-advance-of-commercialization/〉; (accessed 04.02.16.).
  2. Lederle, 2010, Heterofermentative Acetonproduktion (Ph.D. thesis)

Cited by

Sorted by citations

See all 139

Related

Metadata

Authors
Hoffmeister, S., Gerdom, M., Bengelsdorf, F. R., Linder, S., Flüchter, S., Öztürk, H., Blümke, W., May, A., Fischer, R.-J., Bahl, H., & Dürre, P.
Affiliations
  • Universität Ulm (DE)
  • Evonik (Germany) (DE)
  • University of Rostock (DE)
Corresponding author
Hoffmeister, Sabrina
ORCID
Venue
Metabolic Engineering
Publisher
Elsevier BV
Published
2016-07
Volume
36
Pages
37-47
ISSN
1096-7176 (ISSN-L)
DOI
10.1016/j.ymben.2016.03.001
OpenAlex
W2294276789
Cited by
139
References
42
Citations by year
2026: 9 · 2025: 5 · 2024: 12 · 2023: 18 · 2022: 24 · 2021: 17 · 2020: 15 · 2019: 17 · 2018: 6 · 2017: 9 · 2016: 7
FWCI
6.75
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Metabolic Engineering (publishedVersion)
  • PubMed (publishedVersion)
Funders
Bundesministerium für Bildung und Forschung (01RC1105A/C)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Anaerobic Digestion and Biogas Production
Keywords
acetone production, Acetobacterium woodii, CoA transferase, acetoacetate decarboxylase, promoter cloning, acetogenic bacteria, Clostridium acetobutylicum
MeSH terms
Acetates, Acetone, Carbon Dioxide, Recombinant Proteins, Genetic Enhancement, Acetobacterium, Metabolic Networks and Pathways, Biosynthetic Pathways, Metabolic Engineering

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review