Ecological risk assessment for DvSnf7 RNA: A plant-incorporated protectant with targeted activity against western corn rootworm
Open accessAbstract
MON 87411 maize, which expresses DvSnf7 RNA, was developed to provide an additional mode of action to confer protection against corn rootworm (Diabrotica spp.). A critical step in the registration of a genetically engineered crop with an insecticidal trait is performing an ecological risk assessment to evaluate the potential for adverse ecological effects. For MON 87411, an assessment plan was developed that met specific protection goals by characterizing the routes and levels of exposure, and testing representative functional taxa that would be directly or indirectly exposed in the environment. The potential for toxicity of DvSnf7 RNA was evaluated with a harmonized battery of non-target organisms (NTOs) that included invertebrate predators, parasitoids, pollinators, soil biota as well as aquatic and terrestrial vertebrate species. Laboratory tests evaluated ecologically relevant endpoints such as survival, growth, development, and reproduction and were of sufficient duration to assess the potential for adverse effects. No adverse effects were observed with any species tested at, or above, the maximum expected environmental concentration (MEEC). All margins of exposure for NTOs were >10-fold the MEEC. Therefore, it is reasonable to conclude that exposure to DvSnf7 RNA, both directly and indirectly, is safe for NTOs at the expected field exposure levels.
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Studies on numerous non-target species using the dsRNA targeting Snf7 in D. v. virgifera have demonstrated this specificity.
“Studies on numerous non-target species using the dsRNA targeting Snf7 in D. v. virgifera have demonstrated this specificity (Bachmann et al., 2016).”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Bachmann et al., 2016
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Genetically engineered crops help support conservation biological control
Romeis, J., Naranjo, S. E., Meissle, M., & Shelton, A. M. (2019) · Biological Control · doi:10.1016/j.biocontrol.2018.10.001 · Cited by 231 · see citing works
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- Authors
- Bachman, P. M., Huizinga, K. M., Jensen, P. D., Mueller, G., Tan, J., Uffman, J. P., & Levine, S. L.
- Affiliations
- Monsanto (United States) (US)
- Corresponding author
- Bachman, Pamela M.
- Venue
- Regulatory Toxicology and Pharmacology
- Publisher
- Elsevier BV
- Published
- 2016-11
- Volume
- 81
- Pages
- 77-88
- ISSN
- 0273-2300 (ISSN-L)
- OpenAlex
- W2476788712
- Cited by
- 123
- References
- 59
- Citations by year
- 2026: 4 · 2025: 20 · 2024: 16 · 2023: 12 · 2022: 14 · 2021: 18 · 2020: 18 · 2019: 7 · 2018: 6 · 2017: 6 · 2016: 2
- FWCI
- 8.18
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Regulatory Toxicology and Pharmacology (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- Monsanto Company
- California Agricultural Research Inc
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural safety and regulations
- Keywords
- western corn rootworm, Diabrotica, environmental risk assessment, non-target organisms, margin of exposure, non-target invertebrates, pollinators, soil organisms, aquatic vertebrates, terrestrial vertebrates, RNA toxicity
- MeSH terms
- Animals, Coleoptera, Computer Simulation, Zea mays, Environmental Exposure, Humans, Pest Control, Biological, RNA, Double-Stranded, Species Specificity, Time Factors, Gene Expression Regulation, Plant, Crops, Agricultural, Risk Assessment, Toxicity Tests, No-Observed-Adverse-Effect Level, Computational Biology, Databases, Genetic, Plants, Genetically Modified, Food, Genetically Modified, RNA Interference, Host-Pathogen Interactions, Food Safety
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