Characterization of the spectrum of insecticidal activity of a double-stranded RNA with targeted activity against western corn rootworm (Diabrotica virgifera virgifera LeConte)
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The sequence specificity of the endogenous RNA interference pathway allows targeted suppression of genes essential for insect survival and enables the development of durable and efficacious insecticidal products having a low likelihood to adversely impact non-target organisms. The spectrum of insecticidal activity of a 240 nucleotide (nt) dsRNA targeting the Snf7 ortholog in Western Corn Rootworm (WCR; Diabrotica virgifera virgifera) was characterized by selecting and testing insects based upon their phylogenetic relatedness to WCR. Insect species, representing 10 families and 4 Orders, were evaluated in subchronic or chronic diet bioassays that measured potential lethal and sublethal effects. When a specific species could not be tested in diet bioassays, the ortholog to the WCR Snf7 gene (DvSnf7) was cloned and corresponding dsRNAs were tested against WCR and Colorado potato beetle (Leptinotarsa decemlineata); model systems known to be sensitive to ingested dsRNA. Bioassay results demonstrate that the spectrum of activity for DvSnf7 is narrow and activity is only evident in a subset of beetles within the Galerucinae subfamily of Chrysomelidae (>90% identity with WCR Snf7 240 nt). This approach allowed for evaluating the relationship between minimum shared nt sequence length and activity. A shared sequence length of ≥ 21 nt was required for efficacy against WCR (containing 221 potential 21-nt matches) and all active orthologs contained at least three 21 nt matches. These results also suggest that WCR resistance to DvSnf7 dsRNA due to single nucleotide polymorphisms in the target sequence of 240 nt is highly unlikely.
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The GE maize event MON87411 produces a dsRNA targeting the Snf7 protein in D. v. virgifera, which is crucial for the transport of transmembrane proteins.
“This GE maize event (MON87411) produces a dsRNA targeting the Snf7 protein in D. v. virgifera, which is crucial for the transport of transmembrane proteins (Bachman et al., 2013;Bolognesi et al., 2012).”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Bachman et al., 2013
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- Authors
- Bachman, P. M., Bolognesi, R., Moar, W. J., Mueller, G. M., Paradise, M. S., Ramaseshadri, P., Tan, J., Uffman, J. P., Warren, J., Wiggins, B. E., & Levine, S. L.
- Affiliations
- Monsanto (United States) (US)
- Corresponding author
- Bachman, Pamela M.
- Venue
- Transgenic Research
- Published
- 2013-06-08
- Volume
- 22
- Issue
- 6
- Pages
- 1207-1222
- ISSN
- 0962-8819 (ISSN-L)
- 1573-9368
- PMC
- PMC3835954
- OpenAlex
- W2034046707
- Cited by
- 242
- References
- 43
- Citations by year
- 2026: 13 · 2025: 24 · 2024: 22 · 2023: 9 · 2022: 30 · 2021: 24 · 2020: 31 · 2019: 20 · 2018: 11 · 2017: 15 · 2016: 18 · 2015: 12 · 2014: 10 · 2013: 2
- FWCI
- 6.23
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Transgenic Research (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Auburn University
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- RNA interference, double-stranded RNA, western corn rootworm, sequence specificity, non-target organisms, Galerucinae, Chrysomelidae, Colorado potato beetle, single nucleotide polymorphisms, sublethal effects
- MeSH terms
- Animals, Coleoptera, Zea mays, Endotoxins, Insect Control, Larva, RNA, Double-Stranded, Insect Proteins, Plants, Genetically Modified, RNA Interference
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