Validation and quantitation of an in vitro M-cell model
Open accessAbstract
This study has evaluated an in vitro model of the follicle-associated epithelia that overlie Peyer's patches of the small intestine. The model shares many phenotypic characteristics of M cells in vivo. Co-cultures of the human adenocarcinoma cell line Caco-2 and freshly isolated Peyer's patch cells were established. Fluorescence microscopy and quantitative image analysis were used to validate the model against known markers of M-cell phenotype. Apical expression of alkaline phosphatase was down-regulated in co-cultures and villin was re-distributed from the apical membrane to the cytoplasm. alpha5beta1 integrin was found on the apical surfaces of the monolayers and B and T lymphocytes integrated into the monolayers. Particle transport was temperature-dependent in co-cultures, indicating that a transcytotic route was responsible. This model provides opportunities to study factors that influence M-cell development, assess putative Peyer's patch targeting in oral vaccine technologies, and study intestinal uptake in vitro.
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M cells have reduced digestive function because they lack brush-border enzymes such as alkaline phosphatase, which makes them interesting for drug targeting.
“With the loss of brush-border enzymes, such as alkaline phosphatase, M cells show a reduced digestive function, which makes them even more interesting for drug targeting [26,27].”
From Use of lectin-functionalized particles for oral immunotherapy (2012) · cites this paper as [27]
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- Authors
- Tyrer, P., Ruth Foxwell, A., Kyd, J., Harvey, M., Sizer, P., & Cripps, A.
- Affiliations
- University of Canberra (AU)
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2002-12
- Volume
- 299
- Issue
- 3
- Pages
- 377-383
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2043613408
- Cited by
- 51
- References
- 32
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 2 · 2014: 3 · 2013: 4 · 2012: 2
- FWCI
- 0.89
- Type
- journal article
- Language
- en
- Open access
- green
- figshare.com
- Licence
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Acquire (CQUniversity) (submittedVersion)
- Figshare (submittedVersion, cc-by)
- Griffith Research Online (submittedVersion)
- Swinburne figshare (Swinburne University of Technology) (submittedVersion)
- Topics
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Keywords
- M cells, Peyer's patches, follicle-associated epithelium, Caco-2 cells, Peyer's patch cells, alkaline phosphatase, villin, alpha5beta1 integrin, T and B lymphocytes, transcytosis, intestinal absorption, small intestine
- MeSH terms
- Alkaline Phosphatase, Animals, Carrier Proteins, Cells, Cultured, Humans, Intestinal Mucosa, Mice, Inbred BALB C, Microfilament Proteins, Microspheres, Models, Biological, Peyer's Patches, Reproducibility of Results, Lymphocyte Subsets, Coculture Techniques, Cell Culture Techniques, Caco-2 Cells, Integrin alpha5beta1, Mice
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