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Cited by 47Neuroscience and Neuropharmacology ResearchAmino Acid Enzymes and MetabolismNeurotransmitter Receptor Influence on Behavior

Quantitative localization of NMDAR1 receptor subunit immunoreactivity in inferotemporal and prefrontal association cortices of monkey and human

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Huntley, G.W., Vickers, J.C., Morrison, J.H.·1997·Brain Research
DOI 10.1016/s0006-8993(96)00847-5
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Abstract

The cellular and synaptic localization of immunoreactivity for the N-methyl-D-aspartate (NMDA) receptor subunit, NMDAR1, was investigated in inferotemporal and prefrontal association neocortices of monkeys and humans. In all monkey association areas examined, the laminar distribution patterns of NMDAR1 immunoreactivity were similar, and characterized by predominant pyramidal-like neuronal labeling in layers II, III, V and VI and a dense neuropil labeling consisting of intensely stained puncta and fine-caliber processes present throughout layers I-III, and V-VI. Layer IV, in contrast, contained only very lightly immunostained neurons which mostly lacked extensive dendritic staining. The laminar distribution of NMDAR1 immunolabeling in human association cortex was similar to that observed in monkeys. Electron microscopy of monkey areas 46 and TE1 confirmed that intensely immunoreactive asymmetrical postsynaptic densities were present throughout all cell-dense layers of prefrontal and inferotemporal association cortex. Quantitative analyses of the laminar proportions of immunoreactive synapses demonstrated that in both areas examined, the percentages of immunolabeled synapses were mostly similar across superficial layers, layer IV and infragranular layers. Finally, quantitative double-labeling immunofluorescence for non-NMDA receptor subunits or calcium-binding proteins demonstrated that virtually all GluR2/3 or GluR5/6/7-immunoreactive neurons were also labeled for NMDAR1, while regionally-specific subsets of parvalbumin-, calbindin- and calretinin-immunoreactive neurons were co-labeled. These data indicate that in primate association cortex, NMDA receptors are heterogeneously distributed to subsets of functionally distinct types of neurons and subsets of excitatory synapses, suggesting a critical and highly specific role in mediating the activity of excitatory connectivity which converges on cortical association areas.

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  1. Feldman, 1984, Morphology of the neocortical pyramidal neuron, Vol. 5, 123

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Authors
Huntley, G. W., Vickers, J. C., & Morrison, J. H.
Affiliations
  • Icahn School of Medicine at Mount Sinai (US)
  • University of Tasmania (AU)
  • Mount Sinai Hospital (US)
Corresponding author
Huntley, G.W.
ORCID
Venue
Brain Research
Publisher
Elsevier BV
Published
1997-02
Volume
749
Issue
2
Pages
245-262
ISSN
0006-8993 (ISSN-L)
DOI
10.1016/s0006-8993(96)00847-5
OpenAlex
W2135113615
Cited by
47
References
112
Citations by year
2024: 1 · 2020: 2 · 2018: 1 · 2017: 1 · 2016: 1 · 2014: 2 · 2013: 1 · 2012: 2
FWCI
1.31
Type
journal article
Language
en
Open access
Licence
Locations
  • Brain Research (publishedVersion)
  • PubMed (publishedVersion)
  • eCite Digital Repository (University of Tasmania) (submittedVersion)
  • Figshare (submittedVersion, cc-by)
Funders
National Institute on Aging (AG06447, AG 05138, P50AG005138)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
Keywords
NMDA receptor subunits, NMDAR1, association cortex, inferotemporal cortex, prefrontal cortex, laminar distribution, postsynaptic density, excitatory synapses, GluR2/3, parvalbumin, calbindin, calretinin, calcium-binding proteins, pyramidal neurons, monkeys, human, primate neocortex
MeSH terms
Animals, Humans, Immunohistochemistry, Macaca fascicularis, Male, Nerve Tissue Proteins, Neurons, Temporal Lobe, Receptors, N-Methyl-D-Aspartate, Microscopy, Immunoelectron, Prefrontal Cortex, Pyramidal Cells, Calbindins, S100 Calcium Binding Protein G

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