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Formation of hetero-oligomeric complexes of type I and type II receptors for transforming growth factor-beta.

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Yamashita, H., ten Dijke, P., Franzén, P., Miyazono, K., Heldin, C.H.·1994·Journal of Biological Chemistry
DOI 10.1016/s0021-9258(17)32142-7
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Abstract

Transforming growth factor-beta (TGF-beta) transduces signals through a heteromeric complex of type I (T beta R-I) and type II (T beta R-II) TGF-beta receptors. To determine the stoichiometry of this complex we used analysis by affinity labeling with 125I-TGF-beta 1 and covalent cross-linking with disuccinimidyl suberate and immunoprecipitation using T beta R-I- or T beta R-II-specific antisera, followed by two-dimensional gel electrophoresis under nonreducing (first dimension) and reducing (second dimension) conditions. Dimers composed of T beta R-I and/or T beta R-II were observed on mink lung epithelial cells as well as COS-1 cells transfected with T beta R-I and T beta R-II cDNAs. Homodimers of T beta R-I could be demonstrated on these cells after dissociation of T beta R-II by sodium dodecyl sulfate treatment. On the cell surface of mink lung epithelial cell mutants that express only T beta R-II and do not respond to TGF-beta, and on COS-1 cells transfected with only T beta R-II, only homodimers of T beta R-II were seen. The facts that T beta R-I does not bind TGF-beta in the absence of T beta R-II and that T beta R-II forms a signaling complex with T beta R-I, together with our observations that homodimers of T beta R-I and T beta R-II are seen on responsive cells, support the notion that TGF-beta induces the formation of hetero-oligomeric receptor complexes, most likely a heterotetramer containing two molecules each of T beta R-I and T beta R-II.

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Metadata

Authors
Yamashita, H., ten Dijke, P., Franzén, P., Miyazono, K., & Heldin, C. H.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1994-08
Volume
269
Issue
31
Pages
20172-20178
ISSN
0021-9258 (ISSN-L)
DOI
10.1016/s0021-9258(17)32142-7
OpenAlex
W1522242926
Cited by
326
References
0
FWCI
9.44
Type
journal article
Language
en
Open access
hybrid
Licence

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CoChat. (2026). Citation check: Formation of hetero-oligomeric complexes of type I and type II receptors for transforming growth factor-beta. https://app.cochat.ai/papers/10.1016/s0021-9258(17)32142-7
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Yamashita, H., ten Dijke, P., Franzén, P., Miyazono, K., & Heldin, C. H. (1994). Formation of hetero-oligomeric complexes of type I and type II receptors for transforming growth factor-beta. Journal of Biological Chemistry. https://doi.org/10.1016/s0021-9258(17)32142-7

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