Cited by 300Adipose Tissue and MetabolismMitochondrial Function and PathologyPeroxisome Proliferator-Activated Receptors

The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.

Mitochondrial uncoupling protein from mouse brown fat. Molecular cloning, genetic mapping, and mRNA expression

Open access
Jacobsson, A, Stadler, U, Glotzer, M A, Kozak, L P·1985·Journal of Biological Chemistry

Abstract

We have identified cDNAs clones for several cold-inducible mRNAs from the brown adipose tissue of mice. pCIN-1, a plasmid with a 900-base pair insert, encoded the mitochondrial uncoupling protein (UCP) as determined by the ability of the cDNA insert to select, by hybridization, an mRNA that could be translated into a 32,000-Da protein immunoprecipitable with anti-UCP antibodies. Nine tissues were analyzed; however, UCP cDNA hybridized to an mRNA species of 1.6 and 2.0 kilobase pairs only in brown adipose tissue. A maximum induction of 10-fold occurred within 6 h of exposure to cold (5 degrees C). A BamHI restriction fragment polymorphism detected by Southern blot analysis of genomic DNA in recombinant inbred mouse strains allowed us to map the UCP gene to Chromosome 8. The analysis of the UCP gene expression in diabetic (db) and obese (ob) mice maintained at 27 degrees C for 3 days followed by cold exposure for 4 h at 5 degrees C indicated that UCP mRNA levels in mutant mice were unaffected at 27 degrees C and only slightly reduced at 5 degrees C. Accordingly, the inability of diabetic and obese mice to thermoregulate is not associated with a lack of UCP mRNA induction.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/s0021-9258(17)36228-2

Cites

36 of 36 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 36 references

Research works printed but not linked: 6

  1. Krawetz, 1984, Biotechniques, 2, 342
  2. Moutier, 1978, Mouse Newsletter, 58, 64
  3. Sundin, 1980, Comp. Biochem. Biophys., 65B, 463
  4. Nedergaard, 1985, Am. J. Physiol.
  5. Hogan, 1980, Am. J. Physiol., 239, E301
  6. Zaror-Behrens, 1983, Am. J. Physiol., 244, E361

Cited by

Sorted by citations

Items 101–200 of 299

Metadata

Authors
Jacobsson, A., Stadler, U., Glotzer, M. A., & Kozak, L. P.
Affiliations
Stockholm University (SE)
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1985-12
Volume
260
Issue
30
Pages
16250-16254
ISSN
0021-9258 (ISSN-L)
OpenAlex
W1513078811
Cited by
300
References
36
Citations by year
2026: 2 · 2025: 3 · 2024: 2 · 2023: 2 · 2022: 6 · 2021: 10 · 2020: 1 · 2019: 4 · 2018: 8 · 2017: 7 · 2016: 7 · 2015: 1 · 2014: 2 · 2013: 3 · 2012: 3
FWCI
6.62
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • The Mouseion at the JAXlibrary (Jackson Laboratory) (submittedVersion)
Funders
  • National Institutes of Health
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD-08431)
Topics
  • Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
Keywords
mitochondrial uncoupling proteins, brown adipose tissue, cold-responsive genes, cDNA cloning, gene mapping, chromosome 8, mRNA expression, cold exposure, thermoregulation, diabetic mice, recombinant inbred mouse strains, restriction fragment polymorphism
MeSH terms
Uncoupling Protein 1, Animals, Adipose Tissue, Brown, Carrier Proteins, Cloning, Molecular, Cold Temperature, DNA Transposable Elements, DNA, Mitochondrial, Genes, Ion Channels, Membrane Proteins, Mice, Inbred C57BL, Mice, Mutant Strains, Mitochondria, Molecular Weight, RNA, Messenger, Protein Biosynthesis, Mitochondrial Proteins, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review