Lipoprotein binding to canine hepatic membranes. Metabolically distinct apo-E and apo-B,E receptors.
Open accessAbstract
Hepatic membranes from adult dog livers have receptors which bind to lipoproteins containing the E apoprotein (the apo-E HDLc) but lack specific receptors for the apo-B-containing low density lipoproteins (LDL). Scatchard analysis of direct binding data for 125I-apo-E-HDLc revealed nonlinearity of the binding which could be resolved into two components, suggesting the presence of two separate binding sites. The binding site for apo-E HDLc that possessed the highest affinity (Kd = 0.23 x 10(-9) M) was calcium-dependent and was sensitive to proteolytic digestion with pronase. The lower affinity (Kd = 20 x 10(-9) M) binding site for apo-E HDLc did not require calcium and was resistant to pronase digestion. Chemical modification of the arginyl or lysyl residues of the apo-E HDLc prevented the HDLc from binding to the higher affinity receptor but had no effect on their binding to the lower affinity site. Adult canine liver membranes also bound canine 125I-HDL. However, the binding of HDL was of lower affinity (Kd = 8.2 x 10(-8) M), did not require calcium, was not blocked by modification of the lysyl or arginyl residues, and may not be of physiologic significance. Although the liver membranes from normal chow-fed adult dogs did not bind canine LDL, it was possible to demonstrate specific high affinity binding of LDL under certain metabolic conditions in dogs. When adult dogs were treated with the hypocholesterolemic agent cholestyramine, the liver membranes from these animals read…
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The apo E or remnant receptor is found only in hepatic tissues and recognizes particles containing apo E but not apo B.
“The second is the apo E or remnant receptor, which is found in hepatic tissues only and is able to recognize particles containing apo E but not apo B (18).”
From Apolipoprotein E2-Christchurch (136 Arg----Ser). New variant of human apolipoprotein E in a patient with type III hyperlipoproteinemia. (1987) · cites this paper as [18]
Cites
BROWN, M. S., KOVANEN, P. T., & GOLDSTEIN, J. L. (1979) · Proceedings of the 1978 Laurentian Hormone Conference · doi:10.1016/b978-0-12-571135-7.50009-6 · Cited by 311 · see citing works
Sherrill, B. C., & Dietschy, J. M. (1978) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)62331-8 · Cited by 197 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
A Receptor-Mediated Pathway for Cholesterol Homeostasis
Brown, M. S., & Goldstein, J. L. (1986) · Science · doi:10.1126/science.3513311 · Cited by 5,703 · see citing works
Piedrahita, J. A., Zhang, S. H., Hagaman, J. R., Oliver, P. M., & Maeda, N. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.10.4471 · Cited by 944 · see citing works
Human apolipoprotein E. The complete amino acid sequence
Rall, S. C., Weisgraber, K. H., & Mahley, R. W. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)34702-1 · Cited by 741 · see citing works
Self-citationMahley, R. W., & Ji, Z.-S. (1999) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)33334-4 · Cited by 693 · see citing works
Self-citationLipoprotein receptors in the liver. Control signals for plasma cholesterol traffic.
Brown, M. S., & Goldstein, J. L. (1983) · Journal of Clinical Investigation · doi:10.1172/jci111044 · Cited by 545 · see citing works
Hepatic uptake of chylomicron remnants
Cooper, A. D. (1997) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)34932-4 · Cited by 499 · see citing works
Rall, S. C., Weisgraber, K. H., Innerarity, T. L., & Mahley, R. W. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.15.4696 · Cited by 345 · see citing works
Self-citationWeintraub, M. S., Zechner, R., Brown, A., Eisenberg, S., & Breslow, J. L. (1988) · Journal of Clinical Investigation · doi:10.1172/jci113806 · Cited by 331 · see citing works
The receptor-binding domain of human apolipoprotein E. Binding of apolipoprotein E fragments.
Innerarity, T. L., Friedlander, E. J., Rall, S. C., Weisgraber, K. H., & Mahley, R. W. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)44180-9 · Cited by 276 · see citing works
Self-citationWeintraub, M. S., Eisenberg, S., & Breslow, J. L. (1987) · Journal of Clinical Investigation · doi:10.1172/jci112926 · Cited by 261 · see citing works
Diet-induced hypercholesterolemia in mice: prevention by overexpression of LDL receptors
Yokode, M., Hammer, R. E., Ishibashi, S., Brown, M. S., & Goldstein, J. L. (1990) · Science · doi:10.1126/science.2244210 · Cited by 175 · see citing works
Wardell, M. R., Brennan, S. O., Janus, E. D., Fraser, R., & Carrell, R. W. (1987) · Journal of Clinical Investigation · doi:10.1172/jci113096 · Cited by 159 · see citing works
Wang, D., El-Amouri, S. S., Dai, M., Kuan, C.-Y., Hui, D. Y., Brady, R. O., & Pan, D. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1222742110 · Cited by 159 · see citing works
Self-citationFainaru, M., Mahley, R. W., Hamilton, R. L., & Innerarity, T. L. (1982) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)38102-5 · Cited by 156 · see citing works
Self-citationCholesterol uptake in adrenal and gonadal tissues: the SR-BI and “selective” pathway connection
Azhar, S. (2003) · Frontiers in bioscience · doi:10.2741/1165 · Cited by 109 · see citing works
Cladaras, C., Hadzopoulou-Cladaras, M., Felber, B. K., Pavlakis, G., & Zannis, V. I. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)61655-2 · Cited by 92 · see citing works
Vogel, T., Weisgraber, K. H., Zeevi, M. I., Ben-Artzi, H., Levanon, A. Z., Rall, S. C., Innerarity, T. L., Hui, D. Y., Taylor, J. M., & Kanner, D. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.24.8696 · Cited by 88 · see citing works
Self-citationShanmugaratnam, J., Berg, E., Kimerer, L., Johnson, R. J., Amaratunga, A., Schreiber, B. M., & Fine, R. E. (1997) · Molecular Brain Research · doi:10.1016/s0169-328x(97)00176-9 · Cited by 68 · see citing works
Windler, E. E. T., Greeve, J., Daerr, W. H., & Greten, H. (1988) · Biochemical Journal · doi:10.1042/bj2520553 · Cited by 67 · see citing works
Nagata, Y., Chen, J., & Cooper, A. D. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)68158-x · Cited by 54 · see citing works
Role of the Liver in the Degradation of Lipoproteins
Cooper, A. D. (1985) · Gastroenterology · doi:10.1016/s0016-5085(85)80155-4 · Cited by 45 · see citing works
Zannis, V. I., Ordovas, J. M., Cladaras, C., Cole, F. S., Forbes, G., & Schaefer, E. J. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)38808-7 · Cited by 21 · see citing works
ApoE deficiency: Markedly decreased levels of cellular ApoE mRNA
Anchors, J. M., Gregg, R. E., Law, S. W., & Brewer, H. B. (1986) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(86)80510-1 · Cited by 13 · see citing works
- Authors
- Hui, D. Y., Innerarity, T. L., & Mahley, R. W.
- Affiliations
- University of California System (US)
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1981-06
- Volume
- 256
- Issue
- 11
- Pages
- 5646-5655
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1488147530
- Cited by
- 383
- References
- 2
- Citations by year
- 2020: 1 · 2019: 1 · 2018: 2 · 2015: 2 · 2014: 1 · 2013: 3 · 2012: 1
- FWCI
- 85.81
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Keywords
- lipoprotein receptors, canine liver, HDL cholesterol, low-density lipoprotein, receptor binding sites, calcium-dependent binding, cholestyramine, hypocholesterolemia, receptor binding affinity, Scatchard analysis
- MeSH terms
- Animals, Apolipoproteins, Apolipoproteins B, Apolipoproteins E, Calcium, Cell Membrane, Cholestyramine Resin, Dogs, Edetic Acid, Female, Hydrogen-Ion Concentration, Kinetics, Lipoproteins, HDL, Lipoproteins, VLDL, Liver, Male, Receptors, Cell Surface, Low Density Lipoprotein Receptor-Related Protein-1
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
