The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Purification and characterization of Tet(M), a protein that renders ribosomes resistant to tetracycline.
Open accessAbstract
The tet(M) tetracycline resistance gene has been found in a wide variety of clinically important bacteria. It has been shown previously (Burdett, V. (1986) J. Bacteriol. 165, 564-569) that the tet(M) gene product mediates resistance at the level of protein synthesis as judged by in vitro assay. Using this assay, large amounts of protein were purified from an Escherichia coli overproducer expressing the gene under control of a T7 promoter. The purified activity consists of a single polypeptide of molecular weight 68,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and was confirmed to be the tet(M) gene product by amino-terminal sequence analysis. Purified Tet(M) has an associated ribosome-dependent GTPase with the specific activity being similar to that of the corresponding activity associated with elongation factor G. Since Tet(M) also displays substantial homology to elongation factor G throughout its length, Tet(M) may function as an analog of this elongation factor.
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“Accordingly, the RPPs bind and hydrolyze GTP in a ribosome-dependent manner (5, 6, 53), and maintenance of this activity is important for in vivo activity (11, 23).”
From Ribosomal Protection Proteins and Their Mechanism ofTetracyclineResistance (2003) · cites this paper as [5]
In the citing paper
“however, Burdett (5, 6) showed that Tet(M) cannot substitute for the elongation factors in vivo or in vitro.”
From Ribosomal Protection Proteins and Their Mechanism ofTetracyclineResistance (2003) · cites this paper as [5]
Citations
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Molecular Cloning. A Laboratory Manual
Wood, E. (1983) · Biochemical Education · doi:10.1016/0307-4412(83)90068-7 · Cited by 29,534
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
Studier, F. W., & Moffatt, B. A. (1986) · Journal of Molecular Biology · doi:10.1016/0022-2836(86)90385-2 · Cited by 6,385
[2] New M13 vectors for cloning
Messing, J. W. (1983) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(83)01005-8 · Cited by 5,240
Vectors for selective expression of cloned DNAs by T7 RNA polymerase
Rosenberg, A. H., Lade, B. N., Dao-shan, C., Lin, S., Dunn, J. J., & Studier, F. W. (1987) · Gene · doi:10.1016/0378-1119(87)90165-x · Cited by 1,443
Nucleotide sequence of bacteriophage λ DNA
Sanger, F., Coulson, A. R., Hong, G. F., Hill, D. F., & Petersen, G. B. (1982) · Journal of Molecular Biology · doi:10.1016/0022-2836(82)90546-0 · Cited by 1,174
Simple and rapid method for isolating large plasmid DNA from lactic streptococci
Anderson, D. G., & McKay, L. L. (1983) · Applied and Environmental Microbiology · doi:10.1128/aem.46.3.549-552.1983 · Cited by 1,026
GTP-binding domain: three consensus sequence elements with distinct spacing.
Dever, T. E., Glynias, M. J., & Merrick, W. C. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.7.1814 · Cited by 603
Hamel, E., Koka, M., & Nakamoto, T. (1972) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)45679-2 · Cited by 362
Morse, S. A., Johnson, S. R., Biddle, J. W., & Roberts, M. C. (1986) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.30.5.664 · Cited by 314
Conjugative Transposons and the Dissemination of Antibiotic Resistance in Streptococci
Clewell, D. B., & Gawron-Burke, C. (1986) · Annual Review of Microbiology · doi:10.1146/annurev.mi.40.100186.003223 · Cited by 274
A transposon in Streptococcus faecalis with fertility properties
Gawron-Burke, C., & Clewell, D. B. (1982) · Nature · doi:10.1038/300281a0 · Cited by 236
Transposable multiple antibiotic resistance in Streptococcus pneumoniae
Courvalin, P. M., & Carlier, C. (1986) · Molecular and General Genetics MGG · doi:10.1007/bf00430441 · Cited by 218
Separation of three microbial amino acid polymerization factors.
Lucas-Lenard, J. M., & Lipmann, F. A. (1966) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.55.6.1562 · Cited by 187
Heterogeneity of tetracycline resistance determinants in Streptococcus
Burdett, V., Inamine, J. M., & Rajagopalan, S. (1982) · Journal of Bacteriology · doi:10.1128/jb.149.3.995-1004.1982 · Cited by 185
Self-citationMartin, P., Trieu‐Cuot, P., & Courvalin, P. M. (1986) · Nucleic Acids Research · doi:10.1093/nar/14.17.7047 · Cited by 146
Tetracycline-resistant Mycoplasma hominis strains contain streptococcal tetM sequences
Roberts, M. C., Koutsky, L. A., Holmes, K. K., LeBlanc, D. J., & Kenny, G. E. (1985) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.28.1.141 · Cited by 136
Goldman, R. A., Hasan, T., Hall, C. C., Strycharz, W. A., & Cooperman, B. S. (1983) · Biochemistry · doi:10.1021/bi00271a020 · Cited by 134
Sougakoff, W., Papadopoulou, B., Nordmann, P., & Courvalin, P. (1987) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1987.tb02260.x · Cited by 124
Streptococcal tetracycline resistance mediated at the level of protein synthesis
Burdett, V. (1986) · Journal of Bacteriology · doi:10.1128/jb.165.2.564-569.1986 · Cited by 123
Self-citationThe Complete Amino-Acid Sequence of Elongation Factor Tu from Escherichia coli
Jones, M. D., NIELSEN, K. M., Clark, B. F. C., Petersen, T. E., Magnusson, S., Sottrup‐Jensen, L., & Gausing, K. (1980) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1980.tb04748.x · Cited by 120
[47] Preparation of Escherchia coli ribosomal subunits active in polypeptide synthesis
Staehelin, T., & Maglott, D. R. (1971) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(71)20049-5 · Cited by 89
The nucleotide sequence of theEscherichta coli fusgene, coding for elongation factor G
Zengel, J. M., Archer, R. H., & Lindahl, L. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.4.2181 · Cited by 88
Sanchez-Pescador, R., Brown, J. T., Roberts, M., & Urdea, M. S. (1988) · Nucleic Acids Research · doi:10.1093/nar/16.3.1218 · Cited by 87
Gene heterogeneity for tetracycline resistance in Staphylococcus spp
Bismuth, R., Zilhão, R., Sakamoto, H., Guesdon, J.-L., & Courvalin, P. M. (1990) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.34.8.1611 · Cited by 85
Manavathu, E. K., Hiratsuka, K., & Taylor, D. E. (1988) · Gene · doi:10.1016/0378-1119(88)90576-8 · Cited by 85
Hächler, H., Kayser, F. H., & Berger‐Bächi, B. (1987) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.31.7.1033 · Cited by 82
Ohnuki, T., Katoh, T., Imanaka, T., & Aiba, S. (1985) · Journal of Bacteriology · doi:10.1128/jb.161.3.1010-1016.1985 · Cited by 72
Molecular studies on the mechanism of tetracycline resistance mediated by Tet(O)
Manavathu, E. K., Fernandez, C. L., Cooperman, B. S., & Taylor, D. E. (1990) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.34.1.71 · Cited by 72
Bott, K. F., & Wilson, G. A. (1968) · Bacteriological Reviews · doi:10.1128/br.32.4_pt_1.370-378.1968 · Cited by 66
Nucleotide sequence analysis of tetracycline resistance gene tetO from Streptococcus mutans DL5
LeBlanc, D. J., Lee, L. N., Titmas, B. M., Smith, C. J., & Tenover, F. C. (1988) · Journal of Bacteriology · doi:10.1128/jb.170.8.3618-3626.1988 · Cited by 62
Buck, M. A., & Cooperman, B. S. (1990) · Biochemistry · doi:10.1021/bi00474a024 · Cited by 57
The nucleotide sequence of the tetracycline resistance determinant tetM fromUreaplasma urealyticum
Sánchez-Pescador, R., Brown, J. T., Roberts, M. C., & Urdea, M. S. (1988) · Nucleic Acids Research · doi:10.1093/nar/16.3.1216 · Cited by 56
Nucleotide sequence of thetet(M) gene ofTn916
Burdett, V. (1990) · Nucleic Acids Research · doi:10.1093/nar/18.20.6137 · Cited by 49
Self-citationConditional mutations in the translational apparatus of Bacillus subtilis
Dubnau, E., Pifko, S., Sloma, A., Cabane, K., & Smith, I. (1976) · Molecular and General Genetics MGG · doi:10.1007/bf00337929 · Cited by 30
The Conservation of DNA Sequences over Very Long Periods of Evolutionary Time
Filer, D., Dhar, R., & Furano, A. V. (1981) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1981.tb05671.x · Cited by 27
Felicetti, L., Tocchini‐Valentini, G. P., & Matteo, G. F. D. (1969) · Biochemistry · doi:10.1021/bi00836a044 · Cited by 17
Research works printed but not linked: 1
- Burdett, 1987, Plasmid, 17, 79
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Unemo, M., & Shafer, W. M. (2014) · Clinical Microbiology Reviews · doi:10.1128/cmr.00010-14 · Cited by 1,154
Roberts, M. C. (1996) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.1996.tb00251.x · Cited by 689
Aminov, R. I., Garrigues-Jeanjean, N., & Mackie, R. I. (2001) · Applied and Environmental Microbiology · doi:10.1128/aem.67.1.22-32.2001 · Cited by 667
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Metadata
- Authors
- Burdett, V
- Affiliations
- Duke Medical Center (US)
- Corresponding author
- Burdett, V
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1991-02
- Volume
- 266
- Issue
- 5
- Pages
- 2872-2877
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1537838130
- Cited by
- 141
- References
- 39
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 1 · 2023: 2 · 2022: 4 · 2021: 3 · 2020: 5 · 2019: 2 · 2018: 2 · 2017: 5 · 2016: 3 · 2015: 6 · 2014: 6 · 2013: 4 · 2012: 5
- FWCI
- 2.91
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ai015619-18)
- National Institute of Allergy and Infectious Diseases (AI15619)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- tetM, tetracycline resistance, ribosome protection, ribosome-associated GTPase, protein synthesis inhibition, T7 promoter, N-terminal sequence, antibiotic resistance mechanisms
- MeSH terms
- Amino Acid Sequence, Amino Acids, Electrophoresis, Polyacrylamide Gel, Escherichia coli, Genes, Bacterial, Hot Temperature, Hydrolysis, Molecular Sequence Data, Mutation, Peptide Elongation Factor Tu, Peptide Elongation Factors, Plasmids, Repressor Proteins, Ribosomes, Sequence Homology, Nucleic Acid, Tetracycline Resistance, Peptide Elongation Factor G, GTP Phosphohydrolase-Linked Elongation Factors
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