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Interaction of a recombinant form of apolipoprotein[a] with human fibroblasts and with the human hepatoma cell line HepG2

Tam, S P, Zhang, X, Koschinsky, M L·1996·Journal of Lipid Research
DOI 10.1016/s0022-2275(20)37595-7
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Abstract

We have studied the binding, uptake, and degradation of a recombinant form of apolipoprotein[a] (r-apo[a]) using a cultured cell model. In HepG2 cells and in human fibroblasts, r-apo[a] complexed with low density lipoprotein(LDL) is bound and internalized via high affinity (Kd = 10 nM) receptors; in both cell types, low affinity (Kd = 200-300 nM) sites also mediate free apo[a] uptake. Using competition studies, we found that the high affinity binding component corresponds to the LDL receptor. Involvement of the LDL receptor in r-apo[a] uptake by fibroblasts was confirmed using fibroblasts derived from an individual homozygous for familial hypercholesterolemia; in contrast to normal fibroblasts, these cells lacked the high affinity r-apo[a] binding component. Cell association of 125I-labeled r-apo[a] was increased and decreased concomitantly with the up- and down-regulation of the LDL receptor in response to a number of compounds. The addition of alpha 2-macroglobulin as well as treatment with heparinase, chondroitinase ABC, and sodium chlorate did not decrease total specific binding of r-apo[a], suggesting that neither the low density lipoprotein receptor-related protein nor cell surface proteoglycans are involved in r-apo[a] clearance. The low affinity binding component present in both fibroblasts and HepG2 cells likely corresponds to the plasminogen receptor, as binding of r-apo[a] to these sites was specifically decreased by the addition of plasminogen or the lysine analog…

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Research works printed but not linked: 54

  1. Scanu, 1992, Genetic basis and pathophysiological implications of high plasma Lp[a] levels, J Int. Med., 231, 679, 10.1111/j.1365-2796.1992.tb01257.x · DOI
  2. Koschinsky, 1993, Identification of the cysteine residue in apolipoprotein[a] which mediates extracellular coupling with apolipoprotein B-100, J. Biol. Chem., 268, 19819, 10.1016/S0021-9258(19)36587-1 · DOI
  3. White, 1993, Intracellular maturation of apolipoprotein[a] and assembly of lipoprotein[a] in primary baboon hepatocytes, J Lipid Res., 34, 509, 10.1016/S0022-2275(20)40742-4 · DOI
  4. Hajjar, 1989, Lipoprotein[a] modulation of endothelial cell surface fibrinolysis and its potential role in atherosclerosis, Nature., 339, 303, 10.1038/339303a0 · DOI
  5. Miles, 1989, A potential basis for the thrombotic risks associated with lipoprotein[a], Nature., 339, 301, 10.1038/339301a0 · DOI
  6. Harpel, 1989, Plasmin catalyzes binding of lipoproteinfa] to immobilized fibrinogen and fibrin, Proc. Natl. Acad. Sci. USA., 86, 3847, 10.1073/pnas.86.10.3847 · DOI
  7. Rouy, 1992, The binding of human recombinant apolipoprotein[a] and plasminogen to fibrin surfaces, Biochemistry., 31, 6333, 10.1021/bi00142a024 · DOI
  8. Floren, 1981, Uptake of Lp[a] lipoprotein by cultured fibroblasts, Biochem. Bio-phys. Res. Commun., 102, 636, 10.1016/S0006-291X(81)80179-9 · DOI · Paper page
  9. Havekes, 1981, Binding of Lp[a] to the low density lipoprotein receptor of human fibroblasts, FEBS Lett., 132, 169, 10.1016/0014-5793(81)81153-2 · DOI
  10. Krempler, 1983, Studies on the role of specific cell surface receptors in the removal of lipopro-tein[a] in man, J. Clin. Invest., 71, 1431, 10.1172/JCI110896 · DOI · Paper page
  11. Armstrong, 1985, Isolation, characterization, and uptake in human fibroblasts of an apo[a]-free lipoprotein obtained on reduction of lipoproteinfa], J. Lipid Res., 26, 1314, 10.1016/S0022-2275(20)34257-7 · DOI
  12. Steyrer, 1990, Interaction of lipoprotein Lp[a] with the B/E-receptor: a study using isolated bovine adrenal cortex and human fibroblast receptors, J. Lipid Res., 31, 1247, 10.1016/S0022-2275(20)42633-1 · DOI
  13. Hofmann, 1990, Overexpression of human low density lipoprotein receptors leads to accelerated catabolism of Lp[a] lipoprotein in transgenic mice, J. Clin. Invest., 85, 1542, 10.1172/JCI114602 · DOI · Paper page
  14. Snyder, 1994, Binding and degradation of lipo-proteinfa] and LDL by primary cultures of human hepa-tocytes. Comparison with cultured human monocyte-macrophages and fibroblasts, Arterioscler. Thromb., 14, 770, 10.1161/01.ATV.14.5.770 · DOI · Paper page
  15. Kowal, 1989, Low density lipoprotein receptor-related protein mediates uptake of cholesteryl esters derived from apoprotein E-enriched lipoproteins, Proc. Natl. Acad. Sci. USA., 86, 5810, 10.1073/pnas.86.15.5810 · DOI
  16. Kowal, 1990, Opposing effects of apolipoproteins E and C on lipoprotein binding to low density lipoprotein receptor-related protein, J. Biol. Chem., 265, 10771, 10.1016/S0021-9258(18)87014-4 · DOI · Paper page
  17. Beisiegel, 1991, Lipoprotein lipase enhances the binding of chylomicrons to low density lipoprotein receptor-related protein, Proc. Natl. Acad. Sci. USA., 88, 8342, 10.1073/pnas.88.19.8342 · DOI · Paper page
  18. van Leuven, 1986, The receptor-binding domain of human alpha 2-macroglobulin. Isolation after limited proteolysis with a bacterial proteinase, J. Biol. Chem., 261, 11369, 10.1016/S0021-9258(18)67393-4 · DOI
  19. Herz, 1992, LDL receptor-related protein internalizes and degrades uPA-PAI-1 complexes and is essential for embryo implantation, Cell., 71, 411, 10.1016/0092-8674(92)90511-A · DOI · Paper page
  20. März, 1993, Heterogeneous lipoprotein[a] isoforms differ by their interaction with the low density lipoprotein receptor and the low density lipoprotein receptor-related protein/alpha 2-macroglobulin receptor, FEBS Lett., 325, 271, 10.1016/0014-5793(93)81087-G · DOI · Paper page
  21. Miles, 1988, Plasminogen receptors: ubiquitous sites for cellular regulation of Fibrinolysis, Fibrinolysis., 2, 61, 10.1016/0268-9499(88)90369-4 · DOI
  22. Kostner, 1993, Interaction of Lp[a] and of apo[a] with liver cells, Arterioscler. Thromb., 13, 1101, 10.1161/01.ATV.13.7.1101 · DOI · Paper page
  23. Rubenstein, 1972, Interrelationship between rat serum very low density and high density lipoproteins, J. Lipid Res., 13, 317, 10.1016/S0022-2275(20)39393-7 · DOI
  24. Havel, 1955, The distribution and chemical composition of ultracentri-fugally separated lipoproteins in human serum, J. Clin. Invest., 34, 1345, 10.1172/JCI103182 · DOI
  25. Koschinsky, 1991, Apolipo-protein[a]: expression and characterization of a recombinant form of the protein in mammalian cells, Biochemistry., 30, 5044, 10.1021/bi00234a029 · DOI
  26. Knowles, 1982, Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen, Science., 209, 497, 10.1126/science.6248960 · DOI
  27. Tam, 1985, Biphasic effects of estrogen on apolipoprotein synthesis in human hepatoma cells: mechanism of antagonism by testosterone, J. Biol. Chem., 260, 1670, 10.1016/S0021-9258(18)89646-6 · DOI
  28. Tam, S-P. Effect of ethanol on lipoprotein secretion in two human hepatoma cell lines, HepG2 and Hep3B. Alcohol Clin. Exp. Res. 16: 1021-1028. · DOI
  29. McFarlane, 1958, Efficient trace-labelling of protein with iodine, Nature., 182, 53, 10.1038/182053a0 · DOI
  30. Lowry, 1951, Protein measurement with the Folin phenol reagent, J. Biol. Chem., 193, 265, 10.1016/S0021-9258(19)52451-6 · DOI
  31. Beisiegel, 1981, Immunoblot analysis of low density lipoprotein receptors in fibroblasts from subjects with familial hypercholesterolemia, J. Biol. Chem., 256, 11923, 10.1016/S0021-9258(19)68494-2 · DOI · Paper page
  32. Herz, 1990, Low density lipoprotein receptor-related protein mediates endocytosis of monoclonal antibodies in cultured cells and rabbit liver, J. Biol. Chem., 265, 21355, 10.1016/S0021-9258(17)45368-3 · DOI
  33. Stein, 1976, Binding, internalization, and degradation of low density lipoprotein by normal human fibroblasts and by fibroblasts from a case of homozygous familial hypercholesterolemia, Proc. Natl. Acad. Sci. USA., 73, 14, 10.1073/pnas.73.1.14 · DOI
  34. Bierman, 1974, Lipoprotein uptake and metabolism by rat aortic smooth muscle cells in tissue culture, Circ. Res., 35, 136, 10.1161/01.RES.35.1.136 · DOI
  35. Tam, 1978, Fate of apolipoproteins C-II, C-III, and E during lipolysis of human very low density lipoproteins in vitro, J. Lipid Res., 22, 641, 10.1016/S0022-2275(20)37374-0 · DOI
  36. Goldstein, 1976, Release of low density lipoprotein from its cell surface receptor by sulfated glycosaminoglycans, Cell., 7, 85, 10.1016/0092-8674(76)90258-0 · DOI
  37. Ji, 1993, Role of heparan sulfate proteoglycans in the binding and uptake of apolipoprotein E-enriched remnant lipoproteins by cultured cells, J. Biol. Chem., 268, 10160, 10.1016/S0021-9258(18)82186-X · DOI · Paper page
  38. Oike, 1980, Structural analysis of chick-embryo cartilage proteoglycan by selective degradation with chondroitin lysases (chondroitinase) and endo-β-D-galactosidase (keratanase), Biochem.J., 191, 193, 10.1042/bj1910193 · DOI
  39. Rosenthal, 1967, A graphic method for the determination and presentation of binding parameters in a complex system, Anal. Biochem., 20, 525, 10.1016/0003-2697(67)90297-7 · DOI
  40. Goldstein, 1990, Regulation of the mevalonate pathway, Nature., 343, 425, 10.1038/343425a0 · DOI · Paper page
  41. Tam, 1991, Differences between the regulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase and low density lipoprotein receptor in human hepatoma cells and fibroblasts reside primarily at the translational and post-translational levels, J. Biol. Chem., 266, 16764, 10.1016/S0021-9258(18)55366-7 · DOI
  42. Wade, 1988, Hormonal regulation of low-density lipoprotein (LDL) receptor activity in human hepatoma HepG2 cells, Insulin increases LDL receptor activity and diminishes its suppression by exogenous LDL. Eur. J. Biochem., 174, 213
  43. Cohen, 1984, Effects of eom-pactin, mevalonate and low density lipoprotein on 3-hy-droxy-3-methylglutaryl-coenzyme A reductase activity and low-density-lipoprotein-receptor activity in the human hepatoma cell line HepG2, Biochem.J., 222, 35, 10.1042/bj2220035 · DOI
  44. Havekes, 1986, Stimulation of the LDL receptor activity in the human hepatoma cell line HepG2 by high-density serum fractions, Biochim. Biophys. Acta., 875, 236, 10.1016/0005-2760(86)90173-6 · DOI
  45. Havekes, 1987, Cellular free cholesterol in HepG2 cells is only partially available for down-regulation of low-density-lipo-protein receptor activity, Biochem.J., 247, 739, 10.1042/bj2470739 · DOI
  46. Snyder, 1992, Comparative binding and degradation of lipopro-teinfa] and low density lipoprotein by human monocyte-derived macrophages, J. Biol. Chem., 267, 339, 10.1016/S0021-9258(18)48499-2 · DOI
  47. Brown, 1976, Analysis of a mutant strain of human fibroblasts with a defect in the internalization of receptor-bound low density lipoprotein, Cell., 9, 663, 10.1016/0092-8674(76)90130-6 · DOI
  48. Trieu, 1990, Lipoproteinfa] binding to other apolipoprotein B containing lipoproteins, Biochemistry., 29, 5919, 10.1021/bi00477a006 · DOI
  49. Williams, 1992, Mechanisms by which lipoprotein lipase alters cellular metabolism of lipoprotein[a], low density lipoprotein, and nascent lipoproteins, J Biol. Chem., 267, 13284, 10.1016/S0021-9258(18)42208-9 · DOI · Paper page
  50. Herz, 1988, Surface location and high affinity for calcium of a 500-kD liver membrane protein closely related to the LDLrreoeptor suggest a physiological role as lipoprotein receptor, EMBOJ., 7, 4119, 10.1002/j.1460-2075.1988.tb03306.x · DOI
  51. Ichinose, 1986, Localization of the binding site of tissue-type plasminogen activator to fibrin, J. Clin. Invest., 78, 163, 10.1172/JCI112546 · DOI
  52. Gries, 1987, Free and apo B-associated Lp[a]-specific protein in human serum, Clin. Chim. Acta., 164, 93, 10.1016/0009-8981(87)90110-0 · DOI
  53. Menzel, 1990, Abetalipoproteinemia with an apoB-100-lipoprotein[a] glycoprotein complex in plasma, Indication for an assembly defect. J. Biol. Chem., 265, 981
  54. Bottalico, 1993, Cholesterol loading of macrophages leads to marked enhancement of native lipoproteinfa] and apo-protein[a] internalization and degradation, J. Biol. Chem., 268, 8569, 10.1016/S0021-9258(18)52913-6 · DOI

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Metadata

Authors
Tam, S. P., Zhang, X., & Koschinsky, M. L.
Venue
Journal of Lipid Research
Publisher
Elsevier BV
Published
1996-03
Volume
37
Issue
3
Pages
518-533
ISSN
0022-2275
DOI
10.1016/s0022-2275(20)37595-7
Cited by
44
References
1
Language
en
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