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Chapter 11 Autophagy in Neurite Injury and Neurodegeneration
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“In these cell types, free GFP fragments are only detectable in the presence of nonsaturating levels of lysosomotropic compounds (NH4Cl or CQ) or under conditions that attenuate lysosomal acidity; otherwise, the autophagic/degradative machinery appears to be too efficient to allow the accumulation of the proteolytic fragment (Figure 12B,C) [41,65,353].”
From Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1 (2021) · cites this paper as [41]
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“In this case the relative amount of free GFP and the complete fusion protein is the relevant parameter for quantification; although it may not be possible to detect clear changes in the amount of the full-length chimera, especially under conditions of limited flux [41,53].”
From Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1 (2021) · cites this paper as [41]
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Suppression of basal autophagy in neural cells causes neurodegenerative disease in mice
Hara, T., Nakamura, K., Matsui, M., Yamamoto, A., Nakahara, Y., Suzuki-Migishima, R., Yokoyama, M., Mishima, K., Saito, I., Okano, H., et al. (2006) · Nature · doi:10.1038/nature04724 · Cited by 3,882
Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury
Degterev, A., Huang, Z., Boyce, M., Li, Y., Jagtap, P., Mizushima, N., Cuny, G. D., Mitchison, T. J., Moskowitz, M. A., & Yuan, J. (2005) · Nature Chemical Biology · doi:10.1038/nchembio711 · Cited by 3,068
How to Interpret LC3 Immunoblotting
Mizushima, N., & Yoshimori, T. (2007) · Autophagy · doi:10.4161/auto.4600 · Cited by 2,595
Kimura, S., Noda, T., & Yoshimori, T. (2007) · Autophagy · doi:10.4161/auto.4451 · Cited by 2,278
Komatsu, M., Wang, Q. J., Holstein, G. R., Friedrich, V. L., Iwata, J.-I., Kominami, E., Chait, B. T., Tanaka, K., & Yue, Z. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0701311104 · Cited by 614
Kuma, A., Matsui, M., & Mizushima, N. (2007) · Autophagy · doi:10.4161/auto.4012 · Cited by 555 · cited 2×
“Special considerations for protein aggregation disease—A recent study demonstrated that cytosolic protein aggregates can be labeled with GFP-LC3 even in the absence of progression to autophagy (Kuma et al., 2007).”
BackgroundKoike, M., Shibata, M., Waguri, S., Yoshimura, K., Tanida, I., Kominami, E., Gotow, T., Peters, C., von Figura, K., Mizushima, N., et al. (2005) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)61253-9 · Cited by 330
Gong, R., Park, C. S., Abbassi, N. R., & Tang, S.-J. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m512524200 · Cited by 249 · cited 1×
“There is also evidence that mTOR modulates activitydependent synaptic plasticity in the dendrites of hippocampal neurons (Gong et al., 2006), although the specific role of autophagy was not investigated.”
BackgroundAutophagic Stress in Neuronal Injury and Disease
Chu, C. T. (2006) · Journal of Neuropathology & Experimental Neurology · doi:10.1097/01.jnen.0000229233.75253.be · Cited by 211 · cited 1×
“The combined use of early and late AV markers such as GFPLC3 and MDC form a useful complement in studying autophagic maturation (Chu, 2006).”
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Metadata
- Authors
- Chu, C. T., Plowey, E. D., Dagda, R. K., Hickey, R. W., Cherra, S. J., & Clark, R. S. B.
- Venue
- Methods in Enzymology
- Publisher
- Elsevier
- Published
- 2009
- Pages
- 217-249
- ISSN
- 0076-6879
- PMC
- PMC2669321
- Cited by
- 107
- References
- 9
- Language
- en
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