Assessment of cell surface properties and adhesion potential of selected probiotic strains
Abstract
AIM: To evaluate the physicochemical cell surface and adhesive properties of selected probiotic strains for human use. METHODS AND RESULTS: Probiotic strains, Bifidobacterium longum B6, Lactobacillus acidophilus ADH, Lactobacillus paracasei, Lactobacillus rhamnosus GG, Lactobacillus brevis, Lactobacillus casei, Leuconostoc mesenteroides and Pediococcus acidilactici were tested for the physicochemical properties of cell surfaces and the adhesion abilities against foodborne pathogens. Bif. longum B6 (53.6%) and Lact. rhamnosus GG (46.5%) showed the highest hydrophobicity, while the least affinity to xylene was observed in Ped. acidilactici (10.4%). Bifidobacterium longum B6 showed the strongest coaggregation phenotype with Listeria monocytogenes (53.0%), Shigella boydii (42.0%) and Staphylococcus aureus (45.9%). Lactobacillus rhamnosus GG had the strong binding ability to Caco-2 cells and effectively inhibited the adhesion of L. monocytogenes, Salmonella Typhimurium, Sh. boydii and Staph. aureus to Caco-2 cells. The hydrophobicity was highly correlated with coaggregative abilities and competitive inhibition, suggesting a good relationship between in vitro adhesion and in vivo colonization. CONCLUSION: The results suggest that Bif. longum B6 and Lact. rhamnosus GG can be candidate probiotics available for human consumption. SIGNIFICANCE AND IMPACT OF THE STUDY: Because the use of probiotic strains has been more concerned with their beneficial effects in the GI tract, it is essential to examine the potential of probiotic strains based on the physicochemical properties in terms of bacterial-binding and adhesion capabilities.
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L. helveticus 34.9 is a strong electron donor, based on its strong microbial adhesion to chloroform.
“Finally, the strong microbial adhesion to chloroform of L. helveticus 34.9 suggests that this strain is a strong electron donor [42].”
From The Biological Role of the S-Layer Produced by Lactobacillus helveticus 34.9 in Cell Protection and Its Probiotic Properties (2024) · cites this paper as [42]
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“Finally, the strong microbial adhesion to chloroform of L. helveticus 34.9 suggests that this strain is a strong electron donor [42].”
BackgroundInfluential
- Authors
- Xu, H., Jeong, H. S., Lee, H. Y., & Ahn, J.
- Affiliations
- Kangwon National University (KR)
- Corresponding author
- Ahn, J.
- Venue
- Letters in Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-10
- Volume
- 49
- Issue
- 4
- Pages
- 434-442
- ISSN
- 0266-8254 (ISSN-L)
- 1472-765X
- OpenAlex
- W2072004656
- Cited by
- 267
- References
- 34
- Citations by year
- 2026: 14 · 2025: 16 · 2024: 41 · 2023: 19 · 2022: 22 · 2021: 16 · 2020: 29 · 2019: 17 · 2018: 27 · 2017: 9 · 2016: 9 · 2015: 13 · 2014: 14 · 2013: 6 · 2012: 8
- FWCI
- 7.92
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Letters in Applied Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Keywords
- probiotic strains, cell surface hydrophobicity, bacterial adhesion, co-aggregation, competitive inhibition, Lactobacillus rhamnosus GG, foodborne pathogens, Listeria monocytogenes, Staphylococcus aureus, Shigella boydii, Salmonella typhimurium, probiotic colonization, Leuconostoc mesenteroides, Pediococcus acidilactici
- MeSH terms
- Bacteria, Bacterial Adhesion, Humans, Surface Properties, Bacterial Physiological Phenomena, Caco-2 Cells, Probiotics
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