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Frzb, a Secreted Protein Expressed in the Spemann Organizer, Binds and Inhibits Wnt-8
Open accessAbstract
We isolated a Xenopus homolog of Frzb, a newly described protein containing an amino-terminal Frizzled motif. It dorsalized Xenopus embryos and was expressed in the Spemann organizer during early gastrulation. Unlike Frizzled proteins, endogenous Frzb was soluble. Frzb was secretable and could act across cell boundaries. In several functional assays, Frzb antagonized Xwnt-8, a proposed ventralizing factor with an expression pattern complementary to that of Frzb. Furthermore, Frzb blocked induction of MyoD, an action reported recently for a dominant-negative Xwnt-8. Frzb coimmunoprecipitated with Wnt proteins, providing direct biochemical evidence for Frzb-Wnt interactions. These observations implicate Frzb in axial patterning and support the concept that Frzb binds and inactivates Xwnt-8 during gastrulation, preventing inappropriate ventral signaling in developing dorsal tissues.
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“In the extra-cellular matrix, a number of secreted proteins that bind to Wnts and prevent their interaction with either Fz or LRP5/6 to antagonize Wnt signaling have been identified. These include Dickkopf (Dkk) proteins,16 Wnt-inhibitor protein (WIF),17 soluble Frizzled-related proteins (SFRP),18 Cerebrus,19 Frzb20 and the context dependent Wnt inhibitor Wise.21”
From Wnt signal transduction pathways (2008) · cites this paper as [20]
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BackgroundCapdevila, J., & Belmonte, J. C. I. (1999) · Current Opinion in Genetics & Development · doi:10.1016/s0959-437x(99)80065-3 · Cited by 39
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Lupo, G., Harris, W. A., Barsacchi, G., & Vignali, R. (2002) · Development · doi:10.1242/dev.00095 · Cited by 38
Martin‐Manso, G., Calzada, M. J., Chuman, Y., Sipes, J. M., Xavier, C. P., Wolf, V., Kuznetsova, S. A., Rubin, J. S., & Roberts, D. D. (2011) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2011.03.004 · Cited by 38
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Camus, A., & Tam, P. (1999) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/s0070-2153(08)60315-4 · Cited by 40
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Duprez, D., Leyns, L., Bonnin, M.-A., Lapointe, F., Etchevers, H., Robertis, E. M. D., & Douarin, N. M. L. (1999) · Mechanisms of Development · doi:10.1016/s0925-4773(99)00206-3 · Cited by 38
Leung, T., Söll, I., Arnold, S. J., Kemler, R., & Driever, W. (2003) · Developmental Dynamics · doi:10.1002/dvdy.10408 · Cited by 37
“(Leyns et al., 1997; Wang et al., 1997).”
The Spemann organizer and embryonic head induction
Niehrs, C. (2001) · The EMBO Journal · doi:10.1093/emboj/20.4.631 · Cited by 37
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Michiue, T., Fukui, A., Yukita, A., Sakurai, K., Danno, H., Kikuchi, A., & Asashima, M. (2004) · Developmental Dynamics · doi:10.1002/dvdy.20037 · Cited by 36
“Frzb, a secreted protein containing Wnt-interacting domain similar with Frizzled, physically interacts with Wnt and inhibits Wnt signaling (Wang et al., 1997; Leyns et al., 1997).”
BackgroundVertebrate Axial Patterning: From Egg to Asymmetry
Houston, D. W. (2016) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-46095-6_6 · Cited by 36
Nfonsam, L. E., Jandová, J., Jecius, H., Omesiete, P. N., & Nfonsam, V. N. (2019) · World Journal of Gastrointestinal Oncology · doi:10.4251/wjgo.v11.i8.589 · Cited by 35
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Gradl, D., Kühl, M., & Wedlich, D. (1999) · Mechanisms of Development · doi:10.1016/s0925-4773(99)00129-x · Cited by 35
Epigenetic alterations in metastatic cutaneous carcinoma
Darr, O. A. F., Colacino, J. A., Tang, A. L., McHugh, J. B., Bellile, E. L., Bradford, C. R., Prince, M. P., Chepeha, D. B., Rozek, L. S., & Moyer, J. S. (2014) · Head & Neck · doi:10.1002/hed.23701 · Cited by 33
“The protein product of FRZB is secreted and involved in regulation of bone morphogenesis during development(32,33) through antagonism of Wnt signaling.(34,35) Previous studies of the role of FRZB in cancer have identified that overexpression of FRZB is associated with a more differentiated tumor phenotype in gastric c…”
BackgroundKrill, K. T., Gurdziel, K., Heaton, J. H., Simon, D. P., & Hammer, G. D. (2013) · Molecular Endocrinology · doi:10.1210/me.2012-1331 · Cited by 33
“Frzb, a secreted antagonist of Wnt signaling (42, 43), is of particular interest based on the significant role of Wnt/ -catenin signaling in adrenal development.”
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“At later stages of development, Frzb-1 may regulate Wnt activity in the central core region of the limb bud, since Frzb-1 can bind to some Wnts, and antagonize their biological activity (Leyns et al., 1997; Wang et al., 1997).”
BackgroundChen, J., Voigt, J., Gilchrist, M. J., Papalopulu, N., & Amaya, E. (2005) · Mechanisms of Development · doi:10.1016/j.mod.2004.11.008 · Cited by 32
“Among these 13 genes, two (BMP7, Msx1) were previously known to lead to head truncations when missexpressed (Maeda et al., 1997; Suzuki et al., 1997b; Wang et al., 1997a).”
BackgroundXiro‐1 controls mesoderm patterning by repressing bmp‐4 expression in the spemann organizer
Glavic, Á., Gómez-Skármeta, J. L., & Mayor, R. (2001) · Developmental Dynamics · doi:10.1002/dvdy.1189 · Cited by 32
“…while Noggin, Chordin, Frzb, and Cerberus, secreted by the organizer, bind the BMP and Wnt ligands, interfere with their signaling, and promote dorsal fate (Bouwmeester et al., 1996; Piccolo et al., 1997; Zimermann et al., 1996, Wang et al., 1997; Leyns et al., 1997; Hoppler and Moon, 1998).”
BackgroundDetermination, diversification and multipotency of mammalian myogenic cells
Cossu, G., Angelis, L. D., Borello, U., Berarducci, B., Buffa, V., Sonnino, C., Coletta, M., Vivarelli, E., Bouché, M., Lattanzi, L., et al. (2000) · The International Journal of Developmental Biology · doi:10.1387/ijdb.11061434 · Cited by 32
“Another possible level of regulation for Wnt signaling may be exerted by the sFRPs (soluble Frizzled Related Proteins) a new class of genes, recently identified in several laboratories (Leyns et al., 1997; Wang et al., 1997).”
Deletion of frizzled-related protein reduces voluntary running exercise performance in mice
Lories, R. J. U., Peeters, J., Szlufcik, K., Hespel, P., & Luyten, F. P. (2008) · Osteoarthritis and Cartilage · doi:10.1016/j.joca.2008.07.018 · Cited by 30
Self-citationInhibition of canonical Wnt signaling promotes gliogenesis in P0-NSCs
Kunke, D., Bryja, V., Mygland, L., Arenas, E., & Krauss, S. J. K. (2009) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2009.06.084 · Cited by 32
Severe suppression of Frzb/sFRP3 transcription in osteogenic sarcoma
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Ortiz‐Matamoros, A., Salcedo‐Tello, P., Ávila-Muñoz, E., Zepeda, A., & Arias, C. (2013) · Current Neuropharmacology · doi:10.2174/1570159x11311050001 · Cited by 30
Tiedemann, H., Asashima, M., Grunz, H., Knöchel, W., & Tiedemann, H. (1998) · Development Growth & Differentiation · doi:10.1046/j.1440-169x.1998.t01-1-00001.x · Cited by 29
Cell–cell signalling: Frog frizbees
Zorn, A. M. (1997) · Current Biology · doi:10.1016/s0960-9822(06)00248-x · Cited by 30
“[8].”
InfluentialCloning, Sequence Analysis, and Distribution of Rat Metallocarboxypeptidase z
Xin, X., Day, R. W., Dong, W., LEI, Y., & Fricker, L. D. (1998) · DNA and Cell Biology · doi:10.1089/dna.1998.17.311 · Cited by 30
“The Frzb and other frizzled-related proteins are approximately 30 kDa secreted proteins that are thought to bind Wnt/Wing¬ less and block signalling through the frizzled proteins (Leyns et al., 1997; Wang et al., 1997; Rattner et al., 1997; Finch et al., 1997).”
Niehrs, C. (2021) · Developmental Biology · doi:10.1016/j.ydbio.2021.11.006 · Cited by 29
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Sulf1 has ligand-dependent effects on canonical and non-canonical Wnt signalling
Fellgett, S. W., Maguire, R. J., & Pownall, M. E. (2015) · Journal of Cell Science · doi:10.1242/jcs.164467 · Cited by 29
“, 1996), the discovery of secreted frizzled receptors like FzB that antagonise Wnt signalling (Leyns et al., 1997; Wang et al., 1997), and indeed, even the initial description of the canonical and non-canonical pathways was elucidated in Xenopus (Du et al.”
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“…and stained by in situ hybridization for the organizer genes chordin (Sasai et al., 1994), gsc (Cho et al., 1991), noggin (Smith et al., 1991), and Frzb (Leyns et al., 1997; Wang et al., 1997), for the anterior endodermal gene cerberus, and for the mesoderm marker Xbra (Smith et al., 1991) (Fig.”
BackgroundWnt signaling orients the proximal-distal axis of kidney nephrons
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“The expression of Wnt4 (Kispert et al., 1998; Stark et al., 1994) as well as that of the Wnt inhibitors FrzB (Leyns et al., 1997; Wang et al., 1997), Sfrp1 and Sfrp2 (Rattner et al., 1997) was examined at three developmental stages: Stage I before the onset of nephrogenesis, Stage II during the…”
BackgroundInfluentialMomoi, A., Yoda, H., Steinbeißer, H., Fagotto, F., Kondoh, H., Kudō, A., Driever, W., & Furutani‐Seiki, M. (2003) · Mechanisms of Development · doi:10.1016/s0925-4773(03)00003-0 · Cited by 28
The Neural Plate Specifies Somite Size in the Xenopus laevis Gastrula
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Shi, D. (2024) · Journal of Developmental Biology · doi:10.3390/jdb12030020 · Cited by 34
“…several extracellular Wnt antagonists, such as Frzb, Cerberus, and Dickkopf-1, are expressed in the Spemann organizer; they function to protect the Spemann organizer field and promote head development by antagonizing the ventralizing activity of Wnt8 and BMP4 (bone morphogenetic protein 4) [68–72].”
BackgroundRong, X., Chen, C., Zhou, P., Zhou, Y., Li, Y., Lu, L., Liu, Y., Zhou, J., & Duan, C. (2014) · PLoS ONE · doi:10.1371/journal.pone.0099514 · Cited by 27
Over-expression of FRZB in gastric cancer cell suppresses proliferation and induces differentiation
Qu, Y., Li, J., Cai, Q., Wang, Y., Gu, Q., Zhu, Z., & Liu, B. (2007) · Journal of Cancer Research and Clinical Oncology · doi:10.1007/s00432-007-0291-0 · Cited by 27
“Secreted FRZB antagonized the eVects of Xwnt-8 ( Wang et al. 1997; Leyns et al. 1997), Wnt-1 (Leyns et al. 1997) and Wnt-1-induced accumulation of -catenin (Lin et al. 1997).”
Purification and Characterization of Human Metallocarboxypeptidase Z
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Markers of vertebrate mesoderm induction
Stennard, F. A., Ryan, K., & Gurdon, J. B. (1997) · Current Opinion in Genetics & Development · doi:10.1016/s0959-437x(97)80009-3 · Cited by 27
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Cornesse, Y., Pieler, T., & Hollemann, T. (2004) · Developmental Biology · doi:10.1016/j.ydbio.2004.09.016 · Cited by 26
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Kodjabachian, L., Karavanov, A. A., Hikasa, H., Hukriede, N. A., Aoki, T. O., Taira, M., & Dawid, I. B. (2001) · The International Journal of Developmental Biology · doi:10.1387/ijdb.11291848 · Cited by 25
“, 1998) and Frzb (Leyns et al., 1997; Wang et al., 1997), the BMP”
Schlensog, M., Magnus, L., Heide, T., Eschenbruch, J., Steib, F., Tator, M., Kloten, V., Rose, M., Noetzel, E., Gaisa, N. T., et al. (2016) · Epigenetics · doi:10.1080/15592294.2016.1229730 · Cited by 24
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BackgroundTouw, W. A., Ueland, T., Bollerslev, J., Schousboe, J. T., Lim, W. H., Wong, G., Thompson, P. L., Kiel, D. P., Prince, R. L., Rivadeneira, F., et al. (2017) · Journal of the Endocrine Society · doi:10.1210/js.2016-1040 · Cited by 23
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BackgroundSecreted frizzled related proteins inhibit fibrosis in vitro but appear redundant in vivo
Langhe, E. D., Aznar-Lopez, C., Vooght, V. D., Vanoirbeek, J., Luyten, F. P., & Lories, R. J. U. (2014) · Fibrogenesis & Tissue Repair · doi:10.1186/1755-1536-7-14 · Cited by 23
“Frizzled-related protein (FRZB) was the founding member of this family [16-18] and confirmed to bind xWNT8 and antagonize its activity in Xenopus.”
BackgroundSelf-citationEvaluation of SFRP1 as a candidate for human retinal dystrophies.
García‐Hoyos, M., Cantalapiedra, D., Arroyo, C., Esteve, P., Rodríguez, J., Riveiro-Álvarez, R., Trujillo, M. J., Ramos, C., Bovolenta, P., & Ayuso, C. (2004) · PubMed · Cited by 23
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Strapps, W. R., & Tomlinson, A. (2001) · Development · doi:10.1242/dev.128.23.4829 · Cited by 23
Bu, Q., Li, Z., Zhang, J., Xu, F., Liu, J., & Liu, H. (2017) · Journal of Biological Chemistry · doi:10.1074/jbc.m117.791756 · Cited by 20
Cell signaling pathways controlling an axis organizing center in the zebrafish
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The role of the polycystins in kidney development
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Soluble Frizzled-related proteins promote exosome-mediated Wnt re-secretion
Tran, T. H. N., Takada, R., Krayukhina, E., Maruno, T., Mii, Y., Uchiyama, S., & Takada, S. (2024) · Communications Biology · doi:10.1038/s42003-024-05881-8 · Cited by 19
“On the other hand, sFRP proteins were initially thought to act as antagonists, primarily by sequestering Wnt 61 – 63 .”
BackgroundMatricellular proteins in cancer: a focus on secreted Frizzled-related proteins
Vincent, K. M., & Postovit, L.-M. M. (2017) · Journal of Cell Communication and Signaling · doi:10.1007/s12079-017-0398-2 · Cited by 21
Cox, S., Smith, L. B., Bogani, D., Cheeseman, M. T., Siggers, P., & Greenfield, A. (2006) · Molecular Reproduction and Development · doi:10.1002/mrd.20507 · Cited by 21
“Several lines of evidence suggest that SFRPs can antagonize WNT-mediated signaling by direct competitive interaction with WNT ligands via the CRD (Leyns et al., 1997; Wang et al., 1997; Dann et al., 2001) or by formation of nonsignaling complexes with Frizzled proteins (Bafico et al.”
BackgroundInfluentialWhat's your position? the Xenopus cement gland as a paradigm of regional specification
Wardle, F. C., & Sive, H. L. (2003) · BioEssays · doi:10.1002/bies.10294 · Cited by 21
Mahmoud, D. E., Sassi, N., Drissi, G., Barsaoui, M., Zitouna, K., Sahli, H., KALLEL‐SELLAMI, M., Kanoun, L., Cheour, E., & LAADHAR, L. (2017) · Immunological Investigations · doi:10.1080/08820139.2016.1267204 · Cited by 20
“In human, the sFRP family comprises five members, sFRP1–5 (Ekström et al., 2011). sFRP3 binds to Wnt ligands to prevent the activation of the canonical Wnt pathway (Wang et al., 1997).”
BackgroundCadherins and catenins, Wnts and SOXs: Embryonic patterning in Xenopus
Amand, A. L. S., & Klymkowsky, M. W. (2001) · International review of cytology · doi:10.1016/s0074-7696(01)03010-8 · Cited by 20
Interactions between Wingless and Notch during the assignment of cell fates in Drosophila
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“This, together with some circumstantial evidence from naturally occurring dominant negative variants of these receptors in vertebrates (Leyns et al., 1997; Wang et al., 1997), has led to the suggestion that fz genes are receptors for Wingless.”
BackgroundContinuity and change: paradigm shifts in neural induction
Gilbert, S. F. (2001) · The International Journal of Developmental Biology · doi:10.1387/ijdb.11291843 · Cited by 19
“Frzb (“frisbee”) is a small soluble form of the Wnt receptor protein which is capable of binding Wnt proteins in solution (Leyns et al., 1997; Wang et al., 1997).”
Xenopus hairy2b specifies anterior prechordal mesoderm identity within Spemann's organizer
Yamaguti, M., Cho, K. W. Y., & Hashimoto, C. (2005) · Developmental Dynamics · doi:10.1002/dvdy.20523 · Cited by 19
“We also examined the expression of the Wnt antagonists frzb1 (Leyns et al., 1997; Wang et al., 1997 ) and Xdkk1 (Glinka et al., 1998) and the multifunctional (BMP, Wnt, and Nodal) antagonist cerberus (Hsu et al., 1998; Piccolo et al., 1999).”
BackgroundInfluentialArmstrong, A. T., Mandala, A., Malhotra, M., & Gnana‐Prakasam, J. P. (2022) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2022/7163326 · Cited by 18
High-frequency deregulated expression of Wnt signaling pathway members in breast carcinomas
Khan, Z., Arafah, M., Shaik, J. P., Mahale, A. M., & Alanazi, M. A. (2018) · OncoTargets and Therapy · doi:10.2147/ott.s154395 · Cited by 18
Huang, X., Post, J. N., Zhong, L., Leijten, J., Larsson, S., Karperien, M., & Struglics, A. (2018) · Osteoarthritis and Cartilage · doi:10.1016/j.joca.2018.02.904 · Cited by 18
Laux, H., Tomer, R., Mader, M. T., Smida, J., Budczies, J., Kappler, R., Hahn, H., Blöchinger, M., Schnitzbauer, U., Eckardt‐Schupp, F., et al. (2004) · Laboratory Investigation · doi:10.1038/labinvest.3700158 · Cited by 18
Kwon, Y., Chung, Y.-J., Kim, J., Lee, H.-J., Park, J., Roh, T., Cho, H., Yoon, C., Koo, B., & Kim, H.-S. (2014) · PLoS ONE · doi:10.1371/journal.pone.0085736 · Cited by 17
“SFRP1 is one of factors to inhibit both canonical and noncanonical Wnt signaling by reversibly preventing Wnt from interacting with membrane-bound receptors [31,32].”
Asashima, M., Michiue, T., & Kurisaki, A. (2008) · Development Growth & Differentiation · doi:10.1111/j.1440-169x.2008.00990.x · Cited by 17
“In molecular analyses conducted in the 1990s, the head organizer was shown to require several factors, such as Cerberus, frzb, and Dickkopf, for head-inducing activity (Bouwmeester et al. 1996; Leyns et al. 1997; Wang et al. 1997; Glinka et al. 1998).”
FRZBas a key molecule in abdominal aortic aneurysm progression affecting vascular integrity
Oh, C., Ko, Y., Park, J. J., Heo, H. J., Kang, J., Kwon, E. J., Kang, J. W., Lee, Y., Myung, K., Kang, J. M., et al. (2020) · Bioscience Reports · doi:10.1042/bsr20203204 · Cited by 17
Illies, M. R., Peeler, M. T., Dechtiaruk, A., & Ettensohn, C. A. (2002) · Mechanisms of Development · doi:10.1016/s0925-4773(01)00657-8 · Cited by 17
Oldefest, M., Düsterhöft, S., Desel, C., Thysen, S., Fink, C., Rabe, B., Lories, R. J. U., Grötzinger, J., & Lorenzen, I. (2015) · Biochemical Journal · doi:10.1042/bj20141231 · Cited by 16
Multiple maternal influences on dorsal–ventral fate of Xenopus animal blastomeres
Pandur, P., Sullivan, S. A., & Moody, S. A. (2002) · Developmental Dynamics · doi:10.1002/dvdy.10181 · Cited by 16
“Signaling by Wnt1 and Wnt8, but not by Wnt3a, -5a, or -11, can be blocked by the secreted protein Frzb1 (Leyns et al., 1997; Wang et al., 1997a, b).”
BackgroundMethodSiamois functions in the early blastula to induce Spemann's organiser
Kodjabachian, L., & Lemaire, P. (2001) · Mechanisms of Development · doi:10.1016/s0925-4773(01)00484-1 · Cited by 16
Takeda, M., Saito, Y., Sekine, R., Onitsuka, I., Maeda, R., & Maéno, M. (2000) · Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology · doi:10.1016/s0305-0491(00)00194-2 · Cited by 16
Chimeric Wnt proteins define the amino-terminus of Wnt-1 as a transformation-specific determinant
Julius, M. A., Rai, S. D., & Kitajewski, J. (1999) · Oncogene · doi:10.1038/sj.onc.1202268 · Cited by 16
“The CRD is also found within a group of frizzled-related secretory proteins (frps) that includes FrzB-1, an antagonist of Wnt-1 activity in Xenopus and mammalian cells (Finch et al., 1997; Hoang et al., 1996; Leyns et al., 1997; Lin et al., 1997; Rattner et al., 1997; Wang et al., 1997).”
BackgroundFrizzled related protein deficiency impairs muscle strength, gait and calpain 3 levels
Casas-Fraile, L., Cornelis, F. M. F., Costamagna, D., Rico, A., Duelen, R., Sampaolesi, M., de Munaín, A. L., Lories, R. J. U., & Sáenz, A. (2020) · Orphanet Journal of Rare Diseases · doi:10.1186/s13023-020-01372-1 · Cited by 15
Yam, J. W. P., Chan, K. W., Ngan, E., & Hsiao, W. L. W. (2005) · Gene · doi:10.1016/j.gene.2005.05.025 · Cited by 15
“Secreted frizzled related protein (sFrp) homologues have been independently isolated from bovine, Xenopus, mouse, rat and human tissues by different laboratories (Hoang et al., 1996; Leyns et al., 1997; Wang et al., 1997; Rattner et al., 1997; Wolf et al., 1997; Zhou et al., 1998; Chang et al., 1999; Katoh, 2001; Finc…”
BackgroundThe logistics of Wnt production and delivery
Alvarez‐Rodrigo, I., Willnow, D., & Vincent, J. (2023) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/bs.ctdb.2023.01.006 · Cited by 12
The impact of substrate stiffness on morphological, transcriptional and functional aspects in RPE
Wolfram, L., Gimpel, C., Schwämmle, M. E., Clark, S. J., Böhringer, D., & Schlunck, G. (2024) · Scientific Reports · doi:10.1038/s41598-024-56661-7 · Cited by 15
Varshini, M. S., Reddy, R. A., Krishnamurthy, P. T., & Wadhwani, A. (2024) · Neuroscience & Biobehavioral Reviews · doi:10.1016/j.neubiorev.2024.105863 · Cited by 13
Systematic Screening and Expression Analysis of the Head Organizer Genes in Xenopus Embryos
Shibata, M., Itoh, M., Ohmori, S., Shinga, J., & Taira, M. (2001) · Developmental Biology · doi:10.1006/dbio.2001.0428 · Cited by 14
“1 Wnt antagonists include Cerberus (Bouwmeester et al., 1996), Dkk-1 (Glinka et al., 1998), and Frzb-1 (Leyns et al., 1997; Wang et al., 1997a), which are expressed mainly in the head organizer region.”
BackgroundWnt antagonist as therapeutic targets in ovarian cancer
Narayanaa, Y. K., Perumalsamy, N. K., Warrier, S. R., Perumalsamy, L. R., & Dharmarajan, A. (2022) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2022.106191 · Cited by 15
Gao, Y., Li, Q., Du, Z., Yao, Q., Jiang, G., Huang, W., Gao, X., Li, J., Dou, T., Chen, F., et al. (2025) · Journal of Nanobiotechnology · doi:10.1186/s12951-025-03475-y · Cited by 16
“As a well-characterized Wnt signaling antagonist [93], Frzb exerts multiple protective effects on cartilage [94], including: (1) inhibition of inflammatory matrix metalloproteinase production [95]; (2) regulation of developmental signaling pathways [96]; and (3) suppression of tumor invasion [97].”
Inhibition of miR-194 suppresses the Wnt/β-catenin signalling pathway in gastric cancer
Peng, Y., Zhang, X., Lin, H.-J., Deng, S., Huang, Y., Qin, Y., Feng, X.-L., Yan, R., Zhao, Y.-Q., Cheng, Y., et al. (2018) · Oncology Report · doi:10.3892/or.2018.6773 · Cited by 14
“Wnt-associated genes SFRP (29,30), nemo-like kinase (31), pygopus family PHD finger 2 (32,33), FBXW11 (34), PITX2 (35,36), FRAT genes (37) and transducer-like enhancer of split genes (38); signal transducers Dickkopf Wnt signalling pathway inhibitors (39), chibby family member 1, β -catenin antagonist (40) and kringle…”
InfluentialTransport and gradient formation of Wnt and Fgf in the early zebrafish gastrula
Cooper, E. J., & Scholpp, S. (2023) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/bs.ctdb.2023.12.003 · Cited by 12
Ren, H., Luo, J., Ouyang, F., Li, C., Chen, X., Zhou, H., Huang, W., & Zhang, W. (2021) · Frontiers in Cardiovascular Medicine · doi:10.3389/fcvm.2021.675222 · Cited by 12
“protein (FRZB)- a secreted Wnt antagonist (19), secreted frizzled- related proteins (SFRP)- a competitive antagonist of Wnt (20).”
BackgroundZheng, P., Liu, F., Zheng, Z., Pan, H.-W., & Liu, Z. (2023) · Aging · doi:10.18632/aging.204547 · Cited by 12
Pomduk, K., Kheolamai, P., U‐pratya, Y., Wattanapanitch, M., Klincumhom, N., & Issaragrisil, S. (2014) · Annals of Hematology · doi:10.1007/s00277-014-2210-1 · Cited by 12
Wnt/β-Catenin Signaling in Vertebrate Posterior Neural Development
Elkouby, Y. M., & Frank, D. (2010) · Colloquium Series on Developmental Biology · doi:10.4199/c00015ed1v01y201007deb004 · Cited by 12
Kadian, L. K., Yadav, R., Nanda, S., Gulshan, G., Sharma, S., & Yadav, C. (2020) · Molecular Biology Reports · doi:10.1007/s11033-020-05960-z · Cited by 11
Guo, P., Yao, X., Jin, X., Xv, Y., Zhang, J., Li, Q., Yan, C., Li, X., & Kim, N. (2023) · Animals · doi:10.3390/ani13132223 · Cited by 11
“Since SFRPs are homologous to the WNt-binding domain sequence of the Fz receptor, they are considered typical Wnt signaling antagonists that can bind to Wnt proteins and block signal transduction [37].”
BackgroundSpemann-Mangold organizer and mesoderm induction
Asashima, M., & Satou-Kobayashi, Y. (2024) · Cells and Development · doi:10.1016/j.cdev.2024.203903 · Cited by 12
Evaluation of mouse Sfrp3/Frzb1 as a candidate for the lst, Ul, and Far mutants on Chromosome 2
Peichel, C. L., Kozak, C. A., Luyten, F. P., & Vogt, T. (1998) · Mammalian Genome · doi:10.1007/s003359900775 · Cited by 11
Self-citation
Metadata
- Authors
- Wang, S., Krinks, M., Lin, K., Luyten, F. P., & Moos, M.
- Affiliations
- United States Food and Drug Administration (US)
- Center for Biologics Evaluation and Research (US)
- National Institutes of Health (US)
- Corresponding author
- Moos, Malcolm
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1997-03
- Volume
- 88
- Issue
- 6
- Pages
- 757-766
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W1988350691
- Cited by
- 518
- References
- 70
- Citations by year
- 2026: 3 · 2025: 11 · 2024: 8 · 2023: 9 · 2022: 11 · 2021: 6 · 2020: 12 · 2019: 3 · 2018: 13 · 2017: 17 · 2016: 6 · 2015: 12 · 2014: 15 · 2013: 9 · 2012: 14
- FWCI
- 17.08
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- George Washington University
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Health Sciences › Medicine › Oncology › Renal and related cancers
- Keywords
- FRZB, Spemann organizer, dorsalization, ventralization, gastrulation, axial patterning, MyoD, Wnt signaling inhibition, secreted proteins, Xenopus embryo
- MeSH terms
- Frizzled-Related Proteins, Animals, Biological Transport, Cell Communication, Dogs, Embryonic Induction, Female, Glycoproteins, Male, Mesoderm, Microsomes, Protein Binding, Proteins, Rabbits, RNA, Messenger, Solubility, Transcription, Genetic, Xenopus, Zygote, MyoD Protein, Gene Expression Regulation, Developmental, COS Cells, Zebrafish Proteins, Intracellular Signaling Peptides and Proteins, Wnt Proteins
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