The DOI matches its Crossref record; 70 of the 70 references it cites were found in a registry or an index.
Frzb, a Secreted Protein Expressed in the Spemann Organizer, Binds and Inhibits Wnt-8
Open accessAbstract
We isolated a Xenopus homolog of Frzb, a newly described protein containing an amino-terminal Frizzled motif. It dorsalized Xenopus embryos and was expressed in the Spemann organizer during early gastrulation. Unlike Frizzled proteins, endogenous Frzb was soluble. Frzb was secretable and could act across cell boundaries. In several functional assays, Frzb antagonized Xwnt-8, a proposed ventralizing factor with an expression pattern complementary to that of Frzb. Furthermore, Frzb blocked induction of MyoD, an action reported recently for a dominant-negative Xwnt-8. Frzb coimmunoprecipitated with Wnt proteins, providing direct biochemical evidence for Frzb-Wnt interactions. These observations implicate Frzb in axial patterning and support the concept that Frzb binds and inactivates Xwnt-8 during gastrulation, preventing inappropriate ventral signaling in developing dorsal tissues.
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“In the extra-cellular matrix, a number of secreted proteins that bind to Wnts and prevent their interaction with either Fz or LRP5/6 to antagonize Wnt signaling have been identified. These include Dickkopf (Dkk) proteins,16 Wnt-inhibitor protein (WIF),17 soluble Frizzled-related proteins (SFRP),18 Cerebrus,19 Frzb20 and the context dependent Wnt inhibitor Wise.21”
From Wnt signal transduction pathways (2008) · cites this paper as [20]
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Items 501–518 of 518
Bovolenta, P. (2018) · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature · doi:10.3410/f.725544970.793553991
Zi, X., Guo, Y. C., Simoneau, A. R., Hope, C., Xie, J., Holcombe, R. F., & Hoang, B. H. (2023) · doi:10.1158/0008-5472.c.6495890
Zi, X., Guo, Y. C., Simoneau, A. R., Hope, C., Xie, J., Holcombe, R. F., & Hoang, B. H. (2023) · doi:10.1158/0008-5472.c.6495890.v1
Xiao, Z., Zheng, N., Chen, H., Yang, Z., Wang, R., & Liang, Z. (2024) · Translational Psychiatry · doi:10.1038/s41398-024-03056-x · Cited by 1
“In addition, our results did not identify proteins that have a potential causal relationship with BD_II, so studies with larger sample sizes, more tissue sources, and more types of proteins are needed.”
ResultDu, S., Rizou, E. A., & Warmflash, A. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.06.07.658391
“Meanwhile, Wnt antagonists are found in the anterior region and are critical for head induction (Bouwmeester et al., 1996; Glinka et al., 1998; Leyns et al., 1997; Shibata et al., 2005; Wang et al., 1997; Yamamoto et al., 2005).”
Uterine artery transcriptomic signatures of e-cigarette aerosol exposure in pregnancy
Carabulea, A. L., Naik, V. D., Janeski, J. D., Jiang, H., Venkatachalam, S., & Ramadoss, J. (2025) · Reproductive Toxicology · doi:10.1016/j.reprotox.2025.109133
Wnt in Hematopoietic and Leukemic Stem Cells
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Étude du signal véhiculé par les hormones thyroïdiennes dans la physiopathologie intestinale
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Étude du rôle des facteurs de transcription pou3f au cours du développement rénal
Cosse-Etchepare, C. (2017) · theses.fr (ABES) · doi:10.70675/258b85b3z0c5dz4075zb642zd36687fb2f70
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Chen, C.-J., Zhou, L., Chen, H.-T., Barathi, V. A., Qiu, B., Shaik, A. A., Chia, R. N., Lim, S. T., Joo, C., Cheung, N., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-71326-x
“Indeed, FRZB has been shown to form a complex with multiple Wnt ligands and regulate their diffusion and signaling during Xenopus embryo development 30 – 35 .”
Inoue, Y., Kumagai, K., Kim, J., Ishikawa, K., Choe, H., Ike, H., Kobayashi, N., & Inaba, Y. (2026) · Journal of Orthopaedic Research® · doi:10.1002/jor.70295
“Frizzled‐related protein B (FRZB), also known as secreted frizzled‐ related protein 3 (SFRP3), is a critical extracellular antagonist of Wnt/β‐catenin signaling that limits aberrant Wnt pathway activation [13, 14].”
In Vitro Control of Organogenesis by ActivinA Treatment of Amphibian and Mouse Stem Cells
Asashima, M., Kurisaki, A., & Michiue, T. (2008) · Springer eBooks · doi:10.1007/978-1-4020-8274-0_7
“Subsequent molecular analyses clarified that formation of the head organizer requires several secreted factors including Cerberus, frzb and dickkopf (Bouwmeester et al., 1996; Wang et al., 1997; Leyns et al., 1997; Glinka et al., 1998).”
BackgroundControl of Development and Homeostasis Via Regulation of BMP, Wnt, and Hedgehog Signaling
Hackenmiller, R., Degnin, C., & Christian, J. L. (2004) · Humana Press eBooks · doi:10.1007/978-1-59259-736-9_8
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Self-citation
Metadata
- Authors
- Wang, S., Krinks, M., Lin, K., Luyten, F. P., & Moos, M.
- Affiliations
- United States Food and Drug Administration (US)
- Center for Biologics Evaluation and Research (US)
- National Institutes of Health (US)
- Corresponding author
- Moos, Malcolm
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1997-03
- Volume
- 88
- Issue
- 6
- Pages
- 757-766
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W1988350691
- Cited by
- 518
- References
- 70
- Citations by year
- 2026: 3 · 2025: 11 · 2024: 8 · 2023: 9 · 2022: 11 · 2021: 6 · 2020: 12 · 2019: 3 · 2018: 13 · 2017: 17 · 2016: 6 · 2015: 12 · 2014: 15 · 2013: 9 · 2012: 14
- FWCI
- 17.08
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- George Washington University
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Health Sciences › Medicine › Oncology › Renal and related cancers
- Keywords
- FRZB, Spemann organizer, dorsalization, ventralization, gastrulation, axial patterning, MyoD, Wnt signaling inhibition, secreted proteins, Xenopus embryo
- MeSH terms
- Frizzled-Related Proteins, Animals, Biological Transport, Cell Communication, Dogs, Embryonic Induction, Female, Glycoproteins, Male, Mesoderm, Microsomes, Protein Binding, Proteins, Rabbits, RNA, Messenger, Solubility, Transcription, Genetic, Xenopus, Zygote, MyoD Protein, Gene Expression Regulation, Developmental, COS Cells, Zebrafish Proteins, Intracellular Signaling Peptides and Proteins, Wnt Proteins
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