The DOI matches its Crossref record; 70 of the 70 references it cites were found in a registry or an index.
Frzb, a Secreted Protein Expressed in the Spemann Organizer, Binds and Inhibits Wnt-8
Open accessAbstract
We isolated a Xenopus homolog of Frzb, a newly described protein containing an amino-terminal Frizzled motif. It dorsalized Xenopus embryos and was expressed in the Spemann organizer during early gastrulation. Unlike Frizzled proteins, endogenous Frzb was soluble. Frzb was secretable and could act across cell boundaries. In several functional assays, Frzb antagonized Xwnt-8, a proposed ventralizing factor with an expression pattern complementary to that of Frzb. Furthermore, Frzb blocked induction of MyoD, an action reported recently for a dominant-negative Xwnt-8. Frzb coimmunoprecipitated with Wnt proteins, providing direct biochemical evidence for Frzb-Wnt interactions. These observations implicate Frzb in axial patterning and support the concept that Frzb binds and inactivates Xwnt-8 during gastrulation, preventing inappropriate ventral signaling in developing dorsal tissues.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“In the extra-cellular matrix, a number of secreted proteins that bind to Wnts and prevent their interaction with either Fz or LRP5/6 to antagonize Wnt signaling have been identified. These include Dickkopf (Dkk) proteins,16 Wnt-inhibitor protein (WIF),17 soluble Frizzled-related proteins (SFRP),18 Cerebrus,19 Frzb20 and the context dependent Wnt inhibitor Wise.21”
From Wnt signal transduction pathways (2008) · cites this paper as [20]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Antibodies, a laboratory manual
(1989) · Biomedicine & Pharmacotherapy · doi:10.1016/0753-3322(89)90130-3 · Cited by 12,318
Bone: Formation by Autoinduction
Urist, M. R. (1965) · Science · doi:10.1126/science.150.3698.893 · Cited by 5,529
Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites
Nielsen, H., Engelbrecht, J., Brunak, S., & von HEIJNE, G. (1997) · Protein Engineering Design and Selection · doi:10.1093/protein/10.1.1 · Cited by 5,343
Normal Table of Xenopus Laevis (Daudin)
Nieuwkoop, P. D., & Faber, J. (1958) · Copeia · doi:10.2307/1439568 · Cited by 4,542
Wessel, D., & Flügge, U. (1984) · Analytical Biochemistry · doi:10.1016/0003-2697(84)90782-6 · Cited by 3,997
Normal table of Xenopus laevis (Daudin)
Gurdon, J. B. (1995) · Trends in Genetics · doi:10.1016/s0168-9525(00)89129-5 · Cited by 2,888
Appendix G: In Situ Hybridization: An Improved Whole-Mount Method for Xenopus Embryos
Harland, R. M. (1991) · Methods in cell biology · doi:10.1016/s0091-679x(08)60307-6 · Cited by 2,048
Bone morphogenetic proteins: multifunctional regulators of vertebrate development.Preprint
Hogan, B. L. M. (1996) · Genes & Development · doi:10.1101/gad.10.13.1580 · Cited by 1,964
Structural features in eukaryotic mRNAs that modulate the initiation of translation.
Kozak, M. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54860-2 · Cited by 1,826
The Spemann Organizer Signal noggin Binds and Inactivates Bone Morphogenetic Protein 4
Zimmerman, L. B., Jesús-Escobar, J. M. D., & Harland, R. M. (1996) · Cell · doi:10.1016/s0092-8674(00)80133-6 · Cited by 1,639
Research works printed but not linked: 3
- Evans, 1991, Biochemical fractionation of oocytes, Meth. Cell Biol., 36, 117
- Kay, 1991, Injection of oocytes and embryos, Meth. Cell Biol., 36, 657
- Saunders, 1968
Cited by
Sorted by citations
The Wnt Signaling Pathway in Development and Disease
Logan, C. Y., & Nusse, R. (2004) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.20.010403.113126 · Cited by 5,325
“In general, both SFRPs and WIFs are thought to function as extracellular Wnt inhibitors (Bafico et al. 1999, Dennis et al. 1999, Hsieh et al. 1999a, Leyns et al. 1997, Salic et al. 1997, Uren et al. 2000, Wang et al. 1997).”
BackgroundPolakis, P. (2000) · Genes & Development · doi:10.1101/gad.14.15.1837 · Cited by 3,183
“The so-called secreted frizzled-related proteins (sFRP) bind to the wnt ligands, thereby exerting antagonistic activity when overexpressed in wnt signaling assays (Leyns et al. 1997; Wang et al. 1997).”
BackgroundWnt signaling: a common theme in animal development
Cadigan, K. M., & Nusse, R. (1997) · Genes & Development · doi:10.1101/gad.11.24.3286 · Cited by 2,567
“tein does not uniquely bind to cells expressing Dfz2; a variety of other Fz family members (Wang et al. 1996; Y.K. Wang et al. 1997), including the original Fz, also enable cells to interact with Wg (Table 3; Bhanot et al. 1996).”
BackgroundMECHANISMS OF WNT SIGNALING IN DEVELOPMENT
Wodarz, A., & Nusse, R. (1998) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.14.1.59 · Cited by 2,030
“…of Xwnt-5A to induce a secondary axis can be overcome by coinjection of human Frizzled 5, suggesting that the availability of a specific receptor rather than a fundamental difference in its signal transduction P1: APR/ary P2: ARS/dat QC: ARS/APM T1: ARS August 29, 1998 9:42 Annual Reviews AR066-03”
BackgroundDickkopf-1 is a member of a new family of secreted proteins and functions in head induction
Glinka, A., Wu, W., Delius, H., Monaghan, A. P., Blumenstock, C., & Niehrs, C. (1998) · Nature · doi:10.1038/34848 · Cited by 1,608
“Consistent with our two-inhibitor model for head formation, the organizer secretes at least one Wnt-antagonist, Frzb, which blocks muscle formation and anteriorizes embryo”
MethodSecreted antagonists of the Wnt signalling pathway
Kawano, Y., & Kypta, R. (2003) · Journal of Cell Science · doi:10.1242/jcs.00623 · Cited by 1,584
Wnt signal transduction pathways
Komiya, Y., & Habas, R. (2008) · Organogenesis · doi:10.4161/org.4.2.5851 · Cited by 1,528
Wnt signalling and its impact on development and cancer
Klaus, A., & Birchmeier, W. (2008) · Nature Reviews Cancer · doi:10.1038/nrc2389 · Cited by 1,595
The Promise and Perils of Wnt Signaling Through β-Catenin
Moon, R. T., Bowerman, B. A., Boutros, M., & Perrimon, N. (2002) · Science · doi:10.1126/science.1071549 · Cited by 970
Bone Morphogenetic Proteins, Their Antagonists, and the Skeleton
CANALIS, E. M., Economides, A. N., & Gazzerro, E. (2003) · Endocrine Reviews · doi:10.1210/er.2002-0023 · Cited by 863
FORMATION AND FUNCTION OF SPEMANN'S ORGANIZER
Harland, R., & Gerhart, J. (1997) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.13.1.611 · Cited by 839
“though they block the axis-inducing activity of injected wnt mRNA (Hoppler et al 1996, Sokol 1996, Leyns et al 1997, Wang et al 1997); whereas overexpression of GSK3 and depletion of β-catenin does block axis formation.”
BackgroundMcMahon, J. A., Takada, S., Zimmerman, L. B., Fan, C., Harland, R. M., & McMahon, A. P. (1998) · Genes & Development · doi:10.1101/gad.12.10.1438 · Cited by 844
“Four signals have been described that are expressed within the organizer and that have dorsalizing activity: Noggin (Smith and Harland 1992), Follistatin (Hemmati-Brivanlou et al. 1994), Chordin (Sasai et al. 1994), and Frzb (Leyns et al. 1997; Wang et al. 1997).”
BackgroundNovel mechanism of Wnt signalling inhibition mediated by Dickkopf-1 interaction with LRP6/Arrow
Bafico, A., Liu, G., Yaniv, A., Gazit, A., & Aaronson, S. A. (2001) · Nature Cell Biology · doi:10.1038/35083081 · Cited by 772
Wnt signaling in development and tissue homeostasis
Steinhart, Z., & Angers, S. (2018) · Development · doi:10.1242/dev.146589 · Cited by 821
“…proteins that prevent their interaction with the receptor complex, such as Wnt inhibitory factor (WIF) (Hsieh et al., 1999), or secreted frizzled related proteins (sFRPs) (Hsieh et al., 1999; Leyns et al., 1997; Rattner et al., 1997; Wang et al., 1997), which share homology with the Frizzled CRD.”
BackgroundFrzb-1 Is a Secreted Antagonist of Wnt Signaling Expressed in the Spemann Organizer
Leyns, L., Bouwmeester, T., Kim, S., Piccolo, S., & Robertis, E. M. D. (1997) · Cell · doi:10.1016/s0092-8674(00)81921-2 · Cited by 736
Head inducer Dickkopf-1 is a ligand for Wnt coreceptor LRP6
Semënov, M. V., Tamai, K., Brott, B. K., Kühl, M., Sokol, S., & He, X. (2001) · Current Biology · doi:10.1016/s0960-9822(01)00290-1 · Cited by 616
Mechanism and function of signal transduction by the Wnt/β-catenin and Wnt/Ca2+ pathways
Miller, J. R., Hocking, A. M., Brown, J. D., & Moon, R. T. (1999) · Oncogene · doi:10.1038/sj.onc.1203245 · Cited by 689
“…include members of the secreted Frizzled-Related Protein family (called FrzB or FRP; Finch et al., 1997; Leyns et al., 1997; Rattner et al., 1997; Wang et al., 1997), Wnt-inhibitory factor-1 (WIF-1; Hsieh et al., 1999a), Cerberus (Bouwmeester et al., 1996; Piccolo et al., 1999), and Dickkopf…”
BackgroundAxonal Remodeling and Synaptic Differentiation in the Cerebellum Is Regulated by WNT-7a Signaling
Hall, A. C., Lucas, F. R., & Salinas, P. C. (2000) · Cell · doi:10.1016/s0092-8674(00)80689-3 · Cited by 699
Secreted and transmembrane wnt inhibitors and activators
Cruciat, C.-M., & Niehrs, C. (2013) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a015081 · Cited by 646
“1996) and shown to act as a Wnt antagonist (Leyns et al. 1997; Wang et al. 1997).”
BackgroundBovolenta, P., Esteve, P., Ruiz, J. M., Cisneros, E., & López-Rı́os, J. (2008) · Journal of Cell Science · doi:10.1242/jcs.026096 · Cited by 655
“…sequence similarity with the Fz receptors (Hoang et al., 1996; Leyns et al., 1997), and immediately associated with Wnt signalling because of its ability to bind to Wnt8 and block its signalling in Xenopus, strongly supporting its role as a Wnt antagonist (Leyns et al., 1997; Wang et al., 1997).”
BackgroundInfluentialThe oncogenic activation of β-catenin
Polakis, P. G. (1999) · Current Opinion in Genetics & Development · doi:10.1016/s0959-437x(99)80003-3 · Cited by 651
Dickkopf1 Is Required for Embryonic Head Induction and Limb Morphogenesis in the Mouse
Mukhopadhyay, M., Shtrom, S., Rodrı́guez-Esteban, C., Chen, L., Tsukui, T., Gomer, L., Dorward, D. W., Glinka, A., Grinberg, A., Huang, S.-P., et al. (2001) · Developmental Cell · doi:10.1016/s1534-5807(01)00041-7 · Cited by 634
Inhibition of Wnt activity induces heart formation from posterior mesodermPreprint
Marvin, M. J., Rocco, G. D., Gardiner, A., Bush, S. M., & Lassar, A. B. (2001) · Genes & Development · doi:10.1101/gad.855501 · Cited by 590
“As with other FrzB-related Wnt antagonists (Leyns et al. 1997; Salic et al. 1997; Wang et al. 1997; Deardorff et al. 1998; Xu et al. 1998; Itoh and Sokol 1999), injection of crescent RNA into the marginal zone of one cell of a two-cell Xenopus embryo enlarged anterior tissues and inhibited…”
BackgroundInfluentialWnt Signaling and the Polarity of the Primary Body Axis
Petersen, C. P., & Reddien, P. W. (2009) · Cell · doi:10.1016/j.cell.2009.11.035 · Cited by 544
“…cells toward a posterior identity (Figure 2C) (Christian and Moon, 1993; Fredieu et al., 1997; Kiecker and Niehrs, 2001), and decreasing Wnt signaling can push cells toward an anterior identity (Figure 2C) (Leyns et al., 1997; Wang et al., 1997; Glinka et al., 1998; Kiecker and Niehrs, 2001).”
Functional and structural diversity of the human Dickkopf gene family
Krupnik, V. E., Sharp, J., Jiang, C., Robison, K. E., Chickering, T. W., Amaravadi, L. S., Brown, D. E., Guyot, D. J., Mays, G. G., Leiby, K. R., et al. (1999) · Gene · doi:10.1016/s0378-1119(99)00365-0 · Cited by 491
Wnt antagonism initiates cardiogenesis in Xenopus laevisPreprint
Schneider, V., & Mercola, M. A. (2001) · Genes & Development · doi:10.1101/gad.855601 · Cited by 483
Signal transduction by the Wnt family of ligands
Dale, T. C. (1998) · Biochemical Journal · doi:10.1042/bj3290209 · Cited by 467
The establishment of spemann's organizer and patterning of the vertebrate embryo
De Robertis, E. M., Larraín, J., Oelgeschläger, M., & Wessely, O. (2000) · Nature Reviews Genetics · doi:10.1038/35042039 · Cited by 429
“Frzb-1 is a secreted protein that contains a domain similar to the Wnt-binding region of the Frizzled Wnt receptors, and functions by binding to Wnts and antagonizing their activit”
BackgroundWnt5a functions in planar cell polarity regulation in mice
Qian, D., Jones, C., Rzadzinska, A. K., Mark, S., Zhang, X., Steel, K. P., Dai, X., & Chen, P. (2007) · Developmental Biology · doi:10.1016/j.ydbio.2007.03.011 · Cited by 422
“Sfrps can bind Wnts and sequester Wnts from Fz receptors, thus acting as Wnt antagonists (Rattner et al., 1997; Wang et al., 1997).”
BackgroundSecreted Frizzled‐related proteins: searching for relationships and patterns
Jones, S. E., & Jomary, C. (2002) · BioEssays · doi:10.1002/bies.10136 · Cited by 427
Purification and molecular cloning of a secreted, Frizzled-related antagonist of Wnt action
Finch, P. W., He, X., Kelley, M. J., Üren, A., Schaudies, R. P., Popescu, N. C., Rudikoff, S., Aaronson, S. A., VARMUS, H. E., & Rubin, J. S. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.13.6770 · Cited by 417
Wnt signaling controls the fate of mesenchymal stem cells
Ling, L., Nurcombe, V., & Cool, S. M. (2008) · Gene · doi:10.1016/j.gene.2008.12.008 · Cited by 417
“Secreted FZD-related peptides (sFRPs) andWnt inhibitory factor 1 (WIF-1) compete with FZD for Wnt ligand binding, while Dickkopfs (Dkks) and Sclerostin target and antagonize LRPs to block Wnt signaling (Leyns et al., 1997; Wang et al., 1997; Hsieh et al., 1999; Semenov et al., 2001; Mao et al., 2002; Li et al., 2005;…”
BackgroundÜren, A., Reichsman, F., Anest, V., Taylor, W. G., Muraiso, K., Bottaro, D. P., Cumberledge, S., & Rubin, J. S. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.6.4374 · Cited by 408
Funato, Y., Michiue, T., Asashima, M., & Miki, H. (2006) · Nature Cell Biology · doi:10.1038/ncb1405 · Cited by 386
“Pretreatment of cells with sFRP-1 , a secreted Wnt antagonis”
BackgroundA Wnt Survival Guide: From Flies to Human Disease
Chien, A. J., Conrad, W. H., & Moon, R. T. (2009) · Journal of Investigative Dermatology · doi:10.1038/jid.2008.445 · Cited by 382
“Soon after their discovery, studies on these proteins supported the notion that they could bind Wnts and thus act as modulators of Wnt/β-catenin signaling (Lin et al., 1997; Wang et al., 1997a, b; Hoang et al., 1998; Bafico et al., 1999).”
BackgroundTajbakhsh, S., Borello, U., Vivarelli, E., Kelly, R. G., Papkoff, J. S., Duprez, D., BUCKINGHAM, M. E., & Cossu, G. (1998) · Development · doi:10.1242/dev.125.21.4155 · Cited by 390
McLin, V. A., Rankin, S. A., & Zorn, A. M. (2007) · Development · doi:10.1242/dev.001230 · Cited by 377
“By contrast, Wnt-antagonists (Dkk1, Frzb1, Crescent and Sfrp5) secreted by the anterior endoderm (Kemp et al., 2005; Leyns et al., 1997; Pilcher and Krieg, 2002; Wang et al., 1997) would protect it from the posteriorizing Wnt ligands, keep nuclear -catenin levels low, and maintain foregut identity.”
BackgroundBafico, A., Gazit, A., Tata, P., Finch, P. W., Yaniv, A., & Aaronson, S. A. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.23.16180 · Cited by 353
Head induction by simultaneous repression of Bmp and Wnt signalling in Xenopus
Glinka, A., Wu, W., Onichtchouk, D., Blumenstock, C., & Niehrs, C. (1997) · Nature · doi:10.1038/39092 · Cited by 350
Hsieh, J.-C., Rattner, A., Smallwood, P. M., & Nathans, J. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.7.3546 · Cited by 349
Expression Profiling and Functional Analysis of Wnt Signaling Mechanisms in Mesenchymal Stem Cells
Etheridge, S. L., Spencer, G. J., Heath, D. J., & Genever, P. G. (2004) · Stem Cells · doi:10.1634/stemcells.22-5-849 · Cited by 353
Wnt signaling in Xenopus embryos inhibits Bmp4 expression and activates neural developmentPreprint
Baker, J. C., Beddington, R. S. P., & Harland, R. M. (1999) · Genes & Development · doi:10.1101/gad.13.23.3149 · Cited by 337
“Previous work has shown that aminoterminal truncations result in stabilization of b-catenin, likely because of the removal of the proteasome recognition site (Yost et al. 1996; Willert and Nusse 1998; Maniatis 1999).”
BackgroundEstablishment of the Telencephalon during Gastrulation by Local Antagonism of Wnt Signaling
Houart, C., Caneparo, L., Heisenberg, C., Barth, K. A., Takeuchi, M., & Wilson, S. W. (2002) · Neuron · doi:10.1016/s0896-6273(02)00751-1 · Cited by 326
An autocrine mechanism for constitutive Wnt pathway activation in human cancer cells
Bafico, A., Liu, G., Goldin, L., Harris, V., & Aaronson, S. A. (2004) · Cancer Cell · doi:10.1016/j.ccr.2004.09.032 · Cited by 329
“interactions (Leyns et al., 1997; Wang et al., 1997; Bafico et al., 1999, 2001; Mao et al., 2001; Semenov et al., 2001).”
BackgroundInfluentialThe Wnt/calcium pathway activates NF-AT and promotes ventral cell fate in Xenopus embryos
Saneyoshi, T., Kume, S., Amasaki, Y., & Mikoshiba, K. (2002) · Nature · doi:10.1038/417295a · Cited by 322
“Figure 4b shows that both the Xwnt-8 antagonist Frz”
BackgroundSARPs: A family of secreted apoptosis-related proteins
Melkonyan, H. S., Chang, W. C., Shapiro, J. P., Mahadevappa, M., Fitzpatrick, P., Kiefer, M. C., Tomei, L., & Umansky, S. R. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.25.13636 · Cited by 326
Roles of Wnt proteins in neural development and maintenance
Patapoutian, A., & Reichardt, L. F. (2000) · Current Opinion in Neurobiology · doi:10.1016/s0959-4388(00)00100-8 · Cited by 319
Wnt signaling and the activation of myogenesis in mammals
Cossu, G., & Borello, U. (1999) · The EMBO Journal · doi:10.1093/emboj/18.24.6867 · Cited by 312
“Another possible level of regulation of Wnt signaling may be exerted by the sFRPs (soluble Frizzled-related proteins), a new class of genes recently identified in several laboratories (Leyns et al., 1997; Wang et al., 1997).”
BackgroundWnt/Beta-Catenin/Tcf Signaling: A Critical Pathway in Gastrointestinal Tumorigenesis
Kolligs, F. T., Bommer, G. T., & Göke, B. (2002) · Digestion · doi:10.1159/000066755 · Cited by 317
Signaling through ?-catenin and Lef/Tcf
Novak, A., & Dedhar, S. (1999) · Cellular and Molecular Life Sciences · doi:10.1007/s000180050449 · Cited by 305
Marchant, L., Linker, C., Ruiz, P., Guerrero, N., & Mayor, R. (1998) · Developmental Biology · doi:10.1016/s0012-1606(98)80008-0 · Cited by 302
Barrow, J. R., Thomas, K. R., Boussadia, O., Moore, R. H., Kemler, R., Capecchi, M. R., & McMahon, A. P. (2003) · Genes & Development · doi:10.1101/gad.1044903 · Cited by 299
“…derived from the dorsal ectoderm is capable of signaling to the AER of Wnt3 conditional mutant limbs argues against the possible antagonistic action of secreted factors such as frizzled-related proteins (sFRPs), which function by titrating secreted Wnt ligands (Leyns et al. 1997; Wang et al. 1997).”
BackgroundMorphogen Gradient FormationPreprint
Wartlick, O., Kicheva, A., & González‐Gaitán, M. (2009) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a001255 · Cited by 296
“Cite this article as Cold Spring Harb Perspect Biol doi: 10.1101/cshperspect.a001255 (Leyns et al. 1997; Wang et al. 1997).”
BackgroundInfluentialSecreted frizzled-related protein 4 is a potent tumor-derived phosphaturic agent
Berndt, T. J., Craig, T. A., Bowe, A. E., Vassiliadis, J., Reczek, D., Finnegan, R., de Beur, S. M. J., Schiavi, S. C., & Kumar, R. (2003) · Journal of Clinical Investigation · doi:10.1172/jci18563 · Cited by 292
Paralkar, V. M., Vail, A. L., GRASSER, W. A., Brown, T. A., Xu, H., Vukičević, S., Ke, H. Z., Qi, H., Owen, T. A., & Thompson, D. D. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.22.13760 · Cited by 289
Heads or tails: Wnts and anterior–posterior patterning
Yamaguchi, T. P. (2001) · Current Biology · doi:10.1016/s0960-9822(01)00417-1 · Cited by 276
“Subsequent studies showed that Frzb1 is secreted from the organizer, binds Wnts directly, and blocks the formation of secondary axes induced by ectopic XWnt8 when overexpressed in Xenopus [46,47].”
Fedi, P., Bafico, A., Nieto, A., Burgess, W. H., Miki, T., Bottaro, D. P., Kraus, M. H., & Aaronson, S. A. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.27.19465 · Cited by 274
Corin, a Mosaic Transmembrane Serine Protease Encoded by a Novel cDNA from Human Heart
Yan, W., Sheng, N., Seto, M. H., Morser, J., & Wu, Q. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.21.14926 · Cited by 271
Articular cartilage and biomechanical properties of the long bones in Frzb‐knockout mice
Lories, R. J. U., Peeters, J., Bakker, A. D., Tylżanowski, P., Derese, I., Schrooten, J., Thomas, J. T., & Luyten, F. P. (2007) · Arthritis & Rheumatism · doi:10.1002/art.23137 · Cited by 275
Self-citationKremen2 modulates Dickkopf2 activity during Wnt/lRP6 signaling
Mao, B., & Niehrs, C. (2003) · Gene · doi:10.1016/s0378-1119(02)01106-x · Cited by 325
Biochemical interactions in the wnt pathway
Seidensticker, M. J., & Behrens, J. (2000) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/s0167-4889(99)00158-5 · Cited by 260
APCDD1 is a novel Wnt inhibitor mutated in hereditary hypotrichosis simplex
Shimomura, Y., Agalliu, D., Vonica, A., Luria, V., Wajid, M., Baumer, A., Belli, S., Petukhova, L., Schinzel, A., Brivanlou, A. H., et al. (2010) · Nature · doi:10.1038/nature08875 · Cited by 254
Lin, K., Wang, S., Julius, M. A., Kitajewski, J., Moos, M. C., & Luyten, F. P. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.21.11196 · Cited by 251
Self-citationWnt signals from the neural tube block ectopic cardiogenesisPreprint
Tzahor, E., & Lassar, A. B. (2001) · Genes & Development · doi:10.1101/gad.871501 · Cited by 246
“In Drosophila, the BMP family member, dpp (Frasch 1995), and the Wnt family member, wingless (Wu et al. 1995), are required for the maintained expression of the NK homeobox gene tinman and for subsequent cardiogenesis.”
BackgroundTargets, models and challenges in osteoarthritis research
Thysen, S., Luyten, F., & Lories, R. (2015) · Disease Models & Mechanisms · doi:10.1242/dmm.016881 · Cited by 235
“Frizzledrelated protein (FRZB) is a secreted antagonist of the Wnt signalling pathway (Wang et al., 1997) and was originally discovered in a chondrogenic extract of articular cartilage and linked to chondrocyte proliferation (Hoang et al., 1996) (Fig.”
BackgroundSelf-citationWnt signalling: Antagonistic Dickkopfs
Zorn, A. M. (2001) · Current Biology · doi:10.1016/s0960-9822(01)00360-8 · Cited by 238
Qin, K. H., Yu, M. S., Fan, J., Wang, H., Zhao, P., Zhao, G., Zeng, W., Chen, C., Wang, Y., Wang, A., et al. (2023) · Genes & Diseases · doi:10.1016/j.gendis.2023.01.030 · Cited by 243
Leimeister, C., Bach, A., & Gessler, M. (1998) · Mechanisms of Development · doi:10.1016/s0925-4773(98)00072-0 · Cited by 222
“Its mammalian homologues have been cloned and characterized, including a detailed analysis of expression during mouse development (Hoang et al., 1996; Mayr et al., 1997; Wang et al., 1997).”
Tarte, K., Zhan, F., Vos, J. D., Klein, B., & Shaughnessy, J. D. (2003) · Blood · doi:10.1182/blood-2002-10-3161 · Cited by 215
Regulated Endocytic Routing Modulates Wingless Signaling in Drosophila Embryos
Dubois, L., Lecourtois, M., Alexandre, C., Hirst, E., & Vincent, J. (2001) · Cell · doi:10.1016/s0092-8674(01)00375-0 · Cited by 209
Wei, C. L., Miura, T., Robson, P., Lim, S. K., Xu, X., Lee, M. Y., Gupta, S. M., Stanton, L. W., Luo, Y., Schmitt, J., et al. (2005) · Stem Cells · doi:10.1634/stemcells.2004-0162 · Cited by 212
LRP5 and Wnt Signaling: A Union Made for Bone
Johnson, M. L., Harnish, K., Nusse, R., & Hul, W. V. (2004) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.040816 · Cited by 211
Dabdoub, A., Donohue, M. J., Brennan, A., Wolf, V., Montcouquiol, M., Sassoon, D. A., Hseih, J.-C., Rubin, J. S., Salinas, P. C., & Kelley, M. W. (2003) · Development · doi:10.1242/dev.00448 · Cited by 211
Lee, A. Y., He, B., You, L., Dadfarmay, S., Xu, Z., Mazières, J., Mikami, I., McCormick, F. P., & Jablons, D. M. (2004) · Oncogene · doi:10.1038/sj.onc.1207881 · Cited by 209
“…(2004) 23, 6672–6676 & 2004 Nature Publishing Group All rights reserved 0950-9232/04 $30.00 www.nature.com/onc based on their ability to bind and sequester the Wnt ligands, provide an important method for modulating Wnt signaling (Hu et al., 1998; Wang et al., 1997).”
InfluentialGhiglione, C., Carraway, K. L., Amundadottir, L. T., Boswell, R. E., Perrimon, N., & Duffy, J. B. (1999) · Cell · doi:10.1016/s0092-8674(00)80594-2 · Cited by 206
The molecular nature of the zebrafish tail organizer
Agathon, A., Thisse, C., & Thisse, B. (2003) · Nature · doi:10.1038/nature01822 · Cited by 206
“or through overexpression of a Wnt-specific inhibitor, Frz”
Wnt Signalling in Mammalian Development and Cancer
Smalley, M. J., & Dale, T. C. (1999) · Cancer and Metastasis Reviews · doi:10.1023/a:1006369223282 · Cited by 209
“The largest family of such proteins so far discovered are the secreted Frizzled-Related Proteins (sFRP) 1–5 [95–101].”
BackgroundMii, Y., & Taira, M. (2009) · Development · doi:10.1242/dev.032524 · Cited by 205
“, 2008)] and Crescent (Cres) are thought to negatively regulate Wnt signalling to protect the head region from posteriorisation and CEMs by Wnts by opposite concentration gradients of sFRPs against Wnts (Leyns et al., 1997; Wang et al., 1997; Pera and De Robertis, 2000; Shibata et al., 2005).”
BackgroundNalesso, G., Sherwood, J., Bertrand, J., Pap, T., Ramachandran, M., Bari, C. D., Pitzalis, C., & Dell’Accio, F. (2011) · The Journal of Cell Biology · doi:10.1083/jcb.201011051 · Cited by 202
“In addition, a loss of function polymorphism in the SFRP3 gene, encoding for a soluble Wnt inhibitor (Leyns et al., 1997; Wang et al., 1997), has been linked to an increased susceptibility to develop OA (Loughlin et al.”
BackgroundHead in the WNT: the molecular nature of Spemann’s head organizer
Niehrs, C. (1999) · Trends in Genetics · doi:10.1016/s0168-9525(99)01767-9 · Cited by 199
Bang, A. G., Papalopulu, N., Goulding, M., & Kintner, C. R. (1999) · Developmental Biology · doi:10.1006/dbio.1999.9319 · Cited by 197
“frzB and sizzled (szl), which encode secreted proteins that act as antagonists of XWnt-8 signaling, are expressed during gastrulation in dorsal and ventral mesodermal domains, respectively (Leyns et al., 1997; Salic et al., 1997; Wang et al., 1997).”
Connective-tissue growth factor modulates WNT signalling and interacts with the WNT receptor complex
Mercurio, S., Latinkic, B., Itasaki, N., Krumlauf, R. K., & Smith, J. C. (2004) · Development · doi:10.1242/dev.01045 · Cited by 197
Zi, X., Guo, Y., Simoneau, A. R., Hope, C., Xie, J., Holcombe, R. F., & Hoang, B. H. (2005) · Cancer Research · doi:10.1158/0008-5472.can-05-0103 · Cited by 198
“Frzb/sFRP3 binds to both Wnt-8 and Wnt-1 and acts as a functional inhibitor of Wnt-8 activity in Xenopus embryos (17, 18).”
Ligand Receptor Interactions in the Wnt Signaling Pathway inDrosophila
Wu, C.-H., & Nusse, R. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m207850200 · Cited by 190
Enomoto‐Iwamoto, M., Kitagaki, J., Koyama, E., Tamamura, Y., Wu, C., Kanatani, N., Koike, T., Okada, H., Komori, T., Yoneda, T., et al. (2002) · Developmental Biology · doi:10.1006/dbio.2002.0802 · Cited by 188
“All rights reserved. dral ossification (Hartmann and Tabin, 2000) and Wnt-8 is a target of, and is antagonized by, Frzb-1 (Leyns et al., 1997; Wang et al., 1997a).”
Zebrafish Dkk1 Functions in Forebrain Specification and Axial Mesendoderm Formation
Hashimoto, H., Itoh, M., Yamanaka, Y., Yamashita, S., Shimizu, T., Solnica-Krezel, L., Hibi, M., & Hirano, T. (2000) · Developmental Biology · doi:10.1006/dbio.1999.9537 · Cited by 184
Murine Cerberus Homologue mCer-1: A Candidate Anterior Patterning Molecule
Biben, C., Stanley, E. G., Fabri, L., Kotecha, S., Rhinn, M., Drinkwater, C. C., Lah, M. S., Wang, C.-C., Nash, A. D., Hilton, D. J., et al. (1998) · Developmental Biology · doi:10.1006/dbio.1997.8812 · Cited by 185
Yamamoto, A., Nagano, T., Takehara, S., Hibi, M., & Aizawa, S. (2005) · Cell · doi:10.1016/j.cell.2004.11.051 · Cited by 191
“Porcupine (Por)/Mom-1 is required sod et al., 1997; Leyns et al., 1997; Wang et al., 1997; for N-glycosylation and transportation of Wnt ligand (van Glinka et al., 1998; Thisse and Thisse, 1999).”
BackgroundGonzález‐Sancho, J. M., Brennan, K. R., Castelo‐Soccio, L. A., & Brown, A. M. C. (2004) · Molecular and Cellular Biology · doi:10.1128/mcb.24.11.4757-4768.2004 · Cited by 184
“5A) and are presumed to work by preventing interaction of the cognate receptors with ligand (11, 32, 44, 83, 90).”
BackgroundWnt Signaling in Vertebrate Axis SpecificationPreprint
Hikasa, H., & Sokol, S. Y. (2012) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a007955 · Cited by 178
“On the other hand, multiple Wnt antagonists, such as Frzb/Sfrp3, Crescent, Shisa, and Dkk1, are expressed in the head region (Leyns et al. 1997; Wang et al. 1997; Glinka et al. 1998; Shibata et al. 2005; Yamamoto et al. 2005).”
BackgroundWnt signaling in the vasculature
Goodwin, A. M., & D'Amore, P. A. (2002) · Angiogenesis · doi:10.1023/a:1021563510866 · Cited by 178
“FRP-3 binds to and inhibits secondary axisforming activity of Wnt-1 and Wnt-8 in Xenopus embryos [ 49 ], but blocks neither secondary axis activity ofWnt-5a nor downstream gene transcription in response to Wnt-3a or Wnt-11 [50].”
BackgroundAi, L., Tao, Q., Zhong, S., Fields, C. R., Kim, W.-J., Lee, M. W., Cui, Y., Brown, K. D., & Robertson, K. D. (2006) · Carcinogenesis · doi:10.1093/carcin/bgi379 · Cited by 177
BMP-2/-4 and Wnt-8 cooperatively pattern the Xenopus mesoderm
Hoppler, S., & Moon, R. T. (1998) · Mechanisms of Development · doi:10.1016/s0925-4773(98)00004-5 · Cited by 177
“(Leyns et al., 1997; Wang et al., 1997).”
Ugolini, F., Charafe‐Jauffret, E., Bardou, V., Geneix, J., Adélaı̈de, J., Labat-Moleur, F., Penault-Llorca, F. M., Longy, M., Jacquemier, J. D., Birnbaum, D., et al. (2001) · Oncogene · doi:10.1038/sj.onc.1204706 · Cited by 176
“SFRP1 is a member of a group of soluble proteins, the soluble Frizzled-related proteins (SFRPs), also called secreted apoptosis-related proteins (SARPs) (Finch et al., 1997; Leyns et al., 1997; Rattner et al., 1997; Wang et al., 1997).”
BackgroundBaarsma, H. A., Königshoff, M., & Gosens, R. (2013) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2013.01.002 · Cited by 179
“…mutations in the LRP5 or LRP6 resulting in truncated proteins lacking the extracellular domain, induced a constitutively active canonical WNT signal, whereas the lack of the intracellular domain results in dominant negative receptors (G. Liu et al., 2003; Tamai et al., 2000; Wehrli et al., 2000).”
Background5 Cellular Mechanisms of Wingless/Wnt Signal Transduction
Dierick, H. A., & Bejsovec, A. (1998) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/s0070-2153(08)60381-6 · Cited by 170
Anterior Endomesoderm Specification inXenopusby Wnt/β-catenin and TGF-β Signalling Pathways
Zorn, A. M., Butler, K., & Gurdon, J. B. (1999) · Developmental Biology · doi:10.1006/dbio.1999.9257 · Cited by 170
Weinstein, D. C., & Hemmati‐Brivanlou, A. (1999) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.15.1.411 · Cited by 170
Roth, W., Wild‐Bode, C., Platten, M., Grimmel, C., Melkonyan, H. S., DICHGANS, J., & Weller, M. (2000) · Oncogene · doi:10.1038/sj.onc.1203783 · Cited by 169
“However, this signaling pathway can be modulated by a recently identi®ed group of soluble proteins, the soluble Frizzled-related proteins (sFRPs; secreted apoptosis-related proteins, SARPs) (Wang et al., 1997; Leyns et al., 1997; Rattner et al., 1997; Finch et al., 1997; Melkonyan et al., 1997).”
BackgroundHeller, R. S., Dichmann, D. S., Jensen, J. N., Miller, C., Wong, G. G., Madsen, O. D., & Serup, P. (2002) · Developmental Dynamics · doi:10.1002/dvdy.10157 · Cited by 167
“Additionally, in the mouse, four secreted Frizzled-related proteins (sFRPs) have been cloned (Rattner et al., 1997), some of which have been shown to bind Wnts and antagonize Wnt-mediated signaling (Wang et al., 1997a,b; Rattner et al., 1997).”
Background
Metadata
- Authors
- Wang, S., Krinks, M., Lin, K., Luyten, F. P., & Moos, M.
- Affiliations
- United States Food and Drug Administration (US)
- Center for Biologics Evaluation and Research (US)
- National Institutes of Health (US)
- Corresponding author
- Moos, Malcolm
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1997-03
- Volume
- 88
- Issue
- 6
- Pages
- 757-766
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W1988350691
- Cited by
- 518
- References
- 70
- Citations by year
- 2026: 3 · 2025: 11 · 2024: 8 · 2023: 9 · 2022: 11 · 2021: 6 · 2020: 12 · 2019: 3 · 2018: 13 · 2017: 17 · 2016: 6 · 2015: 12 · 2014: 15 · 2013: 9 · 2012: 14
- FWCI
- 17.08
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- George Washington University
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Health Sciences › Medicine › Oncology › Renal and related cancers
- Keywords
- FRZB, Spemann organizer, dorsalization, ventralization, gastrulation, axial patterning, MyoD, Wnt signaling inhibition, secreted proteins, Xenopus embryo
- MeSH terms
- Frizzled-Related Proteins, Animals, Biological Transport, Cell Communication, Dogs, Embryonic Induction, Female, Glycoproteins, Male, Mesoderm, Microsomes, Protein Binding, Proteins, Rabbits, RNA, Messenger, Solubility, Transcription, Genetic, Xenopus, Zygote, MyoD Protein, Gene Expression Regulation, Developmental, COS Cells, Zebrafish Proteins, Intracellular Signaling Peptides and Proteins, Wnt Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
