Cited by 518Wnt/β-catenin signaling in development and cancerDevelopmental Biology and Gene RegulationRenal and related cancers

The DOI matches its Crossref record; 70 of the 70 references it cites were found in a registry or an index.

Frzb, a Secreted Protein Expressed in the Spemann Organizer, Binds and Inhibits Wnt-8

Open access

Wang, Shouwen, Krinks, Marie, Lin, Keming, Luyten, Frank P, Moos, Malcolm·1997·Cell

DOI 10.1016/s0092-8674(00)81922-4

Open this paper in CoChat — free account Open PDF

Abstract

We isolated a Xenopus homolog of Frzb, a newly described protein containing an amino-terminal Frizzled motif. It dorsalized Xenopus embryos and was expressed in the Spemann organizer during early gastrulation. Unlike Frizzled proteins, endogenous Frzb was soluble. Frzb was secretable and could act across cell boundaries. In several functional assays, Frzb antagonized Xwnt-8, a proposed ventralizing factor with an expression pattern complementary to that of Frzb. Furthermore, Frzb blocked induction of MyoD, an action reported recently for a dominant-negative Xwnt-8. Frzb coimmunoprecipitated with Wnt proteins, providing direct biochemical evidence for Frzb-Wnt interactions. These observations implicate Frzb in axial patterning and support the concept that Frzb binds and inactivates Xwnt-8 during gastrulation, preventing inappropriate ventral signaling in developing dorsal tissues.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1016/s0092-8674(00)81922-4

Cites

70 of 70 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Antibodies, a laboratory manual
(1989) · Biomedicine & Pharmacotherapy · doi:10.1016/0753-3322(89)90130-3 · Cited by 12,318
Bone: Formation by Autoinduction
Urist, M. R. (1965) · Science · doi:10.1126/science.150.3698.893 · Cited by 5,529
Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites
Nielsen, H., Engelbrecht, J., Brunak, S., & von HEIJNE, G. (1997) · Protein Engineering Design and Selection · doi:10.1093/protein/10.1.1 · Cited by 5,343
Normal Table of Xenopus Laevis (Daudin)
Nieuwkoop, P. D., & Faber, J. (1958) · Copeia · doi:10.2307/1439568 · Cited by 4,542
A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids.
Wessel, D., & Flügge, U. (1984) · Analytical Biochemistry · doi:10.1016/0003-2697(84)90782-6 · Cited by 3,997
Normal table of Xenopus laevis (Daudin)
Gurdon, J. B. (1995) · Trends in Genetics · doi:10.1016/s0168-9525(00)89129-5 · Cited by 2,888
Appendix G: In Situ Hybridization: An Improved Whole-Mount Method for Xenopus Embryos
Harland, R. M. (1991) · Methods in cell biology · doi:10.1016/s0091-679x(08)60307-6 · Cited by 2,048
Bone morphogenetic proteins: multifunctional regulators of vertebrate development.Preprint
Hogan, B. L. M. (1996) · Genes & Development · doi:10.1101/gad.10.13.1580 · Cited by 1,964
Structural features in eukaryotic mRNAs that modulate the initiation of translation.
Kozak, M. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54860-2 · Cited by 1,826
The Spemann Organizer Signal noggin Binds and Inactivates Bone Morphogenetic Protein 4
Zimmerman, L. B., Jesús-Escobar, J. M. D., & Harland, R. M. (1996) · Cell · doi:10.1016/s0092-8674(00)80133-6 · Cited by 1,639

Show all 70 references

Research works printed but not linked: 3

Evans, 1991, Biochemical fractionation of oocytes, Meth. Cell Biol., 36, 117
Kay, 1991, Injection of oocytes and embryos, Meth. Cell Biol., 36, 657
Saunders, 1968

Sorted by citations

Items 201–300 of 518

Somite formation and patterning
Hirsinger, E., Jouve, C., Dubrulle, J., & Pourquié, O. (2000) · International review of cytology · doi:10.1016/s0074-7696(00)98002-1 · Cited by 74
Wnt signaling in castration-resistant prostate cancer: implications for therapy.
Yokoyama, N. N., Shao, S., Hoang, B. H., Mercola, D., & Zi, X. (2014) · PubMed Central · Cited by 73
Secreted frizzled related protein 4 (sFRP4) update: A brief review
Pawar, N. M., & Rao, P. (2018) · Cellular Signalling · doi:10.1016/j.cellsig.2018.01.019 · Cited by 72
Wnt Signaling and Mammary Tumorigenesis
Smalley, M. J., & Dale, T. C. (2001) · Journal of Mammary Gland Biology and Neoplasia · doi:10.1023/a:1009564431268 · Cited by 71
Secreted Frizzled-related protein-2 (sFRP2) augments canonical Wnt3a-induced signaling
von Marschall, Z., & Fisher, L. W. (2010) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2010.08.043 · Cited by 71
“Because of their sequence homology with the Wnt-binding domain of the Fz receptors, sFRPs have been considered antagonists of canonical Wnt signaling by binding to Wnt proteins and preventing signal transduction [16;17].”
Background
Requirement for anti-dorsalizing morphogenetic protein in organizer patterning
Dosch, R., & Niehrs, C. (2000) · Mechanisms of Development · doi:10.1016/s0925-4773(99)00245-2 · Cited by 71
“Whole-mount in situ hybridisation shows that admp is expressed in chordamesoderm overlapping with chordin unlike frzb and dkk1, which are predominantly expressed in leading edge endomesoderm and prechordal plate (Leyns et al., 1997; Wang et al., 1997b; Glinka et al., 1998) (Fig. 6B).”
DDC‐4, an apoptosis‐associated gene, is a secreted frizzled relative
Wolf, V., Ke, G., Dharmarajan, A., Bielke, W., Artuso, L., Saurer, S., & Friis, R. R. (1997) · FEBS Letters · doi:10.1016/s0014-5793(97)01324-0 · Cited by 71
The Dynamic Expression Pattern of frzb-1 Suggests Multiple Roles in Chick Development
Baranski, M., Berdougo, E., Sandler, J. S., Darnell, D. K., & Burrus, L. W. (2000) · Developmental Biology · doi:10.1006/dbio.1999.9516 · Cited by 70
Inverse correlation of secreted frizzled‐related protein 4 and β‐catenin expression in endometrial stromal sarcomas
Hrzenjak, A., Tippl, M., Kremser, M., Strohmeier, B., Guelly, C., Neumeister, D., Lax, S., Moinfar, F., Tabrizi, A. D., Isadi‐Moud, N., et al. (2004) · The Journal of Pathology · doi:10.1002/path.1616 · Cited by 69
The mouse Secreted frizzled-related protein 5 gene is expressed in the anterior visceral endoderm and foregut endoderm during early post-implantation development
Finley, K. R., Tennessen, J. M., & Shawlot, W. (2003) · Gene Expression Patterns · doi:10.1016/s1567-133x(03)00091-7 · Cited by 69
“Cell culture studies and gain of function experiments using Xenopus embryos indicate that Sfrp proteins can bind Wnt molecules extracellularly and interfere with Wnt signaling (Leyns et al., 1997; Wang et al., 1997; Rattner et al., 1997; Chang et al., 1999).”
Background
Neural induction in Xenopus requires inhibition of Wnt-β-catenin signaling
Heeg‐Truesdell, E., & LaBonne, C. (2006) · Developmental Biology · doi:10.1016/j.ydbio.2006.06.015 · Cited by 68
The Netrin-related domain of Sfrp1 interacts with Wnt ligands and antagonizes their activity in the anterior neural plate
López-Rı́os, J., Esteve, P., Ruiz, J. M., & Bovolenta, P. (2008) · Neural Development · doi:10.1186/1749-8104-3-19 · Cited by 68
“In contrast, the strong anteriorisation observed after Sfrp1 and Sfrp1NTR over-expression could be easily explained as the result of an early and generalized antagonism of the canonical Wnt pathway, since inhibition of this pathway induces similar anteriorised and dorsalised phenotypes in both fish and Xenopus embryos…”
BackgroundInfluential
Secreted Frizzled Related Protein 1 Is Overexpressed in Uterine Leiomyomas, Associated with a High Estrogenic Environment and Unrelated to Proliferative Activity
Fukuhara, K., Kariya, M., Kita, M., Shime, H., Kanamori, T., 小阪, 千., Orii, A., Fujita, J., & Fujii, S. (2002) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem.87.4.8375 · Cited by 68
Identification of the laminar-inducing factor: Wnt-signal from the anterior rim induces correct laminar formation of the neural retina in vitro
Nakagawa, S., Takada, S., Takada, R., & Takeichi, M. (2003) · Developmental Biology · doi:10.1016/s0012-1606(03)00320-8 · Cited by 68
Foregut endoderm is required at head process stages for anteriormost neural patterning in chick
Withington, S. L., Beddington, R. S. P., & Cooke, J. (2001) · Development · doi:10.1242/dev.128.3.309 · Cited by 66
Function of zebrafish β-catenin and TCF-3 in dorsoventral patterning
Pelegri, F. J., & Maischein, H. (1998) · Mechanisms of Development · doi:10.1016/s0925-4773(98)00132-4 · Cited by 67
The history of osteoarthritis-osteoarthrosis
Dequeker, J., & Luyten, F. P. (2007) · Annals of the Rheumatic Diseases · doi:10.1136/ard.2007.079764 · Cited by 66
Self-citation
Pharmacological interventions in the Wnt pathway: inhibition of Wnt secretion versus disrupting the protein–protein interfaces of nuclear factors
Zimmerli, D., Hausmann, G., Cantù, C., & Basler, K. (2017) · British Journal of Pharmacology · doi:10.1111/bph.13864 · Cited by 65
“Additionally, there is evidence that Pygo’s chromatin binding ability is required for mammary gland outgrowth (Watanabe et al., 2014).”
Background
Expression of Xfz3, a Xenopus frizzled family member, is restricted to the early nervous system
Shi, D., Goisset, C., & Boucaut, J. (1998) · Mechanisms of Development · doi:10.1016/s0925-4773(97)00166-4 · Cited by 66
Wnt modulation in bone healing
Schupbach, D., Comeau-Gauthier, M., Harvey, E., & Merle, G. (2020) · Bone · doi:10.1016/j.bone.2020.115491 · Cited by 60
Phosphatonins: a new class of phosphate-regulating proteins
Schiavi, S. C., & Moe, O. W. (2002) · Current Opinion in Nephrology & Hypertension · doi:10.1097/00041552-200207000-00009 · Cited by 63
An Endothelial Cell Niche Induces Hepatic Specification Through Dual Repression of Wnt and Notch Signaling
Han, S., Dziedzic, N., Gadue, P. J., Keller, G. M., & Gouon–Evans, V. (2010) · Stem Cells · doi:10.1002/stem.576 · Cited by 62
Hox gene expression in a single Caenorhabditis elegans cell is regulated by a caudal homolog and intercellular signals that inhibit Wnt signaling
Hunter, C. P., Harris, J. M., Maloof, J., & Kenyon, C. J. (1999) · Development · doi:10.1242/dev.126.4.805 · Cited by 62
Roles of two types of heparan sulfate clusters in Wnt distribution and signaling in Xenopus
Mii, Y., Yamamoto, T., Takada, R., Mizumoto, S., Matsuyama, M., Yamada, S., Takada, S., & Taira, M. (2017) · Nature Communications · doi:10.1038/s41467-017-02076-0 · Cited by 60
The role of morphogens in T-cell development
Varas, A. (2003) · Trends in Immunology · doi:10.1016/s1471-4906(03)00033-4 · Cited by 61
Molecular Genetic Studies of Wnt Signaling in the Mouse
Uusitalo, M., Heikkilä, M. M., & Vainio, S. J. (1999) · Experimental Cell Research · doi:10.1006/excr.1999.4710 · Cited by 61
FrzB-2: a human secreted frizzled-related protein with a potential role in chondrocyte apoptosis
James, I. E., Kumar, S., Barnes, M. R., Gress, C. J., Hand, A. T., Dodds, R. A., Connor, J. R., Bradley, B. R., Campbell, D. A., Grabill, S. E., et al. (2000) · Osteoarthritis and Cartilage · doi:10.1053/joca.1999.0321 · Cited by 61
Review Article: Is Wnt Signaling an Attractive Target for the Treatment of Osteoarthritis?
Lories, R. J. U., & Monteagudo, S. (2020) · Rheumatology and Therapy · doi:10.1007/s40744-020-00205-8 · Cited by 60
“like heparin-sulfated proteoglycans [10], and their binding to other secreted molecules such as those belonging to the secreted frizzled related protein (SFRP) family that can serve as molecular shuttles [11, 12], are factors that impact the signaling range of Wnts.”
Background
The Wnt antagonists Frzb-1 and Crescent locally regulate basement membrane dissolution in the developing primary mouth
DICKINSON, A. J. G., & Sive, H. L. (2009) · Development · doi:10.1242/dev.032912 · Cited by 60
Roles of secreted frizzled-related proteins in cancer
Shi, Y. H., Biao, H. E., You, L., & Jablons, D. M. (2007) · Acta Pharmacologica Sinica · doi:10.1111/j.1745-7254.2007.00692.x · Cited by 60
Activation of β–catenin-LEF/TCF signal pathway in chondrocytes stimulates ectopic endochondral ossification
Kitagaki, J., Iwamoto, M., Liu, J.-G., Tamamura, Y., Pacifci, M., & Enomoto‐Iwamoto, M. (2002) · Osteoarthritis and Cartilage · doi:10.1053/joca.2002.0863 · Cited by 61
Wnt-1 signaling inhibits human umbilical vein endothelial cell proliferation and alters cell morphology
Cheng, C.-W., Smith, S. K., & Jones, D. S. (2003) · Experimental Cell Research · doi:10.1016/j.yexcr.2003.07.006 · Cited by 60
Multiple Roles of sFRP2 in Cardiac Development and Cardiovascular Disease
Wu, Y., Liu, X., Zheng, H., Zhu, H., Mai, W., Huang, X., & Huang, Y. (2020) · International Journal of Biological Sciences · doi:10.7150/ijbs.40923 · Cited by 59
“The sFRP family is thought to be a suppressor of Wnt signaling [10-12].”
Background
Different Activities of the Frizzled-Related Proteins frzb2 and sizzled2 during Xenopus Anteroposterior Patterning
Bradley, L., Sun, B. I., Collins‐Racie, L. A., LaVallie, E., McCoy, J. M., & Sive, H. L. (2000) · Developmental Biology · doi:10.1006/dbio.2000.9873 · Cited by 59
Role of Goosecoid, Xnot and Wnt antagonists in the maintenance of the notochord genetic programme inXenopusgastrulae
Yasuo, H., & Lemaire, P. (2001) · Development · doi:10.1242/dev.128.19.3783 · Cited by 59
Alterations of frizzled (FzE3) and secreted frizzled related protein (hsFRP) expression in gastric cancer
To, K. F., Chan, M. W. Y., Leung, W. K., Yu, J. Q., TONG, J. H. M., Lee, T., Chan, F. K. L., & Sung, J. J. �. (2001) · Life Sciences · doi:10.1016/s0024-3205(01)01422-9 · Cited by 58
The Interaction between the Drosophila Secreted Protein Argos and the Epidermal Growth Factor Receptor Inhibits Dimerization of the Receptor and Binding of Secreted Spitz to the Receptor
Jin, M.-H., Sawamoto, K., Ito, M., & Okano, H. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.6.2098-2107.2000 · Cited by 57
SFRP1 and SFRP2 Dose‐Dependently Regulate Midbrain Dopamine Neuron Development In Vivo and in Embryonic Stem Cells
Kele, J., Andersson, E. R., Villaescusa, C., Čajánek, L., Parish, C. L., Bonilla, S., Toledo, E. M., Bryja, V., Rubin, J. S., Shimono, A., et al. (2012) · Stem Cells · doi:10.1002/stem.1049 · Cited by 57
Patterning the early sea urchin embryo
Ettensohn, C. A., & Sweet, H. C. (2000) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/s0070-2153(00)50002-7 · Cited by 57
Elevated levels of β-catenin and fibronectin in three-dimensional collagen cultures of Dupuytren's disease cells are regulated by tension in vitro
Howard, J. C., Varallo, V. M., Ross, D. C., Roth, J. H., Faber, K. J., Alman, B. A., & Gan, B. S. (2003) · BMC Musculoskeletal Disorders · doi:10.1186/1471-2474-4-16 · Cited by 56
Xenopus leads the way: Frogs as a pioneering model to understand the human brain
Exner, C. R. T., & Willsey, H. R. (2020) · genesis · doi:10.1002/dvg.23405 · Cited by 57
Cloning and embryonic expression of five distinct sfrp genes in the zebrafish Danio rerio
Tendeng, C., & Houart, C. (2006) · Gene Expression Patterns · doi:10.1016/j.modgep.2006.01.006 · Cited by 56
Identification and Cloning of a Secreted Protein Related to the Cysteine-Rich Domain of Frizzled
Duplàa, C., Jaspard‐Vinassa, B., Moreau, C., & D'Amore, P. A. (1999) · Circulation Research · doi:10.1161/01.res.84.12.1433 · Cited by 56
Involvement of NLK and Sox11 in neural induction in Xenopus development
Hyodo‐Miura, J., Urushiyama, S., Nagai, S., Nishita, M., Ueno, N., & Shibuya, H. (2002) · Genes to Cells · doi:10.1046/j.1365-2443.2002.00536.x · Cited by 61
“Inhibitors of Wnt signalling, such as Frzb (Leyns et al. 1997; Wang et al. 1997 ), Dkk1 (Glinka et al. 1998), and Wif1 (Hsieh et al. 1999) can co-operate with BMP inhibitors to induce the formation of secondary heads containing forebrain and eyes.”
Background
Adenomatous Polyposis Coli/β-Catenin Interaction and Downstream Targets: Altered Gene Expression in Gastrointestinal Tumors
Clements, W. M., Lowy, A. M., & Groden, J. L. (2003) · Clinical Colorectal Cancer · doi:10.3816/ccc.2003.n.018 · Cited by 55
Specification of anteroposterior axis by combinatorial signaling during Xenopus development
Carron, C., & Shi, D. (2015) · Wiley Interdisciplinary Reviews Developmental Biology · doi:10.1002/wdev.217 · Cited by 59
Wnt inhibitory factor (WIF)-1 inhibits osteoblastic differentiation in mouse embryonic mesenchymal cells
Cho, S. W., Yang, J., Sun, H. J., Jung, J. Y., Her, S. J., Cho, H. Y., Choi, H. J., Kim, S. W., Kim, S. Y., & Shin, C. S. (2009) · Bone · doi:10.1016/j.bone.2009.02.012 · Cited by 55
“Overexpression of hWIF-1 in Xenopus embryos inhibits the activity of XWnt8 and Xwnt3a in a dose-dependentmanner [12] and results in phenotype closely resembling that of Frzb (sFRP-3) overexpression [13].”
Background
WNT signalling events near the cell membrane and their pharmacological targeting for the treatment of cancer
Driehuis, E., & Clevers, H. C. (2017) · British Journal of Pharmacology · doi:10.1111/bph.13758 · Cited by 55
“Wise was discovered in Xenopus embryos as a negative regulator of the WNT pathway.”
Background
Molecular Cloning and Characterization of Human Frizzled-4 on Chromosome 11q14-q21
Kirikoshi, H., Sagara, N., Koike, J., Tanaka, K., Sekihara, H., Hirai, M., & Katoh, M. (1999) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1999.1612 · Cited by 55
Inhibition of myoblast differentiation by Sfrp1 and Sfrp2
Descamps, S., Arzouk, H., Bacou, F., Bernardi, H., Fédon, Y., Gay, S., Reyne, Y., Rossano, B., & Levin, J. M. (2008) · Cell and Tissue Research · doi:10.1007/s00441-008-0574-z · Cited by 56
“…domain of unknown function and a cysteine-rich domain (CRD) that possesses a high degree of structural and sequence similarity to the CRD of Frizzled receptors (Finch et al. 1997; Hoang et al. 1996; Leyns et al. 1997; Melkonyan et al. 1997; Rattner et al. 1997; Salic et al. 1997; Wang et al. 1997).”
Background
Primary body axes of vertebrates: Generation of a near‐Cartesian coordinate system and the role of Spemann‐type organizer
Meinhardt, H. (2006) · Developmental Dynamics · doi:10.1002/dvdy.20952 · Cited by 54
Secreted Frizzled Related Protein 2 (sFRP2) Decreases Susceptibility to UV-Induced Apoptosis in Primary Culture of Canine Mammary Gland Tumors by NF-κB Activation or JNK Suppression
Lee, J.-L., Chang, C., Chueh, L.-L., & Lin, C. (2006) · Breast Cancer Research and Treatment · doi:10.1007/s10549-006-9233-9 · Cited by 54
The role of the homeodomain protein Bozozok in zebrafish axis formation
Solnica‐Krezel, L., & Driever, W. (2001) · The International Journal of Developmental Biology · doi:10.1387/ijdb.11291860 · Cited by 54
“Among those factors are Frzb, bearing similarity to a putative Wnt binding domain of the Frizzled receptors (Leyns et al., 1997; Moon et al., 1997; Wang et al., 1997); Dickkopf1 (Glinka et al.”
Pluripotent cells (stem cells) and their determination and differentiation in early vertebrate embryogenesis†
Tiedemann, H., Asashima, M., Grunz, H., & Knöchel, W. (2001) · Development Growth & Differentiation · doi:10.1046/j.1440-169x.2001.00599.x · Cited by 54
“Fritz can bind and inhibit Wnt-8 and is expressed in all three germ layers in early gastrulation (Leyns et al. 1997; Mayr et al. 1997; Wang et al. 1997).”
Background
Early and dynamic expression of cSfrp1 during chick embryo development
Esteve, P., Morcillo, J., & Bovolenta, P. (2000) · Mechanisms of Development · doi:10.1016/s0925-4773(00)00421-4 · Cited by 54
“Additionally, cSfrp1 transcripts were found in the axial and lateral mesoderm, the otic placode, the trigeminal ganglia, the mesoderm of the branchial arches, the developing limb buds, as well as in the mesodermal component of the developing kidney. q 2000 Elsevier Science Ireland Ltd.”
Background
Patterning of the mesoderm involves several threshold responses to BMP-4 and Xwnt-8
Marom, K., Fainsod, A., & Steinbeißer, H. (1999) · Mechanisms of Development · doi:10.1016/s0925-4773(99)00137-9 · Cited by 53
Frizzled-7 receptor ectodomain expression in a colon cancer cell line induces morphological change and attenuates tumor growth
Vincan, E., Darcy, P. K., Smyth, M. J., Ramsay, R. G., Thompson, E. W., Thomas, R. J. S., & Phillips, W. A. (2005) · Differentiation · doi:10.1111/j.1432-0436.2005.00015.x · Cited by 52
Inhibition of Wnt Signaling by Exogenous Mfrzbl Protein Affects Molar Tooth Size
Sarkar, L., & Sharpe, P. T. (2000) · Journal of Dental Research · doi:10.1177/00220345000790040601 · Cited by 52
Snail2 controls mesodermal BMP/Wnt induction of neural crest
Shi, J., Severson, C., Yang, J., Wedlich, D., & Klymkowsky, M. W. (2011) · Development · doi:10.1242/dev.064394 · Cited by 51
“6J) (Leyns et al., 1997; Wang et al., 1997a; Wang et al., 1997b) and to increases in the levels of RNAs encoding the BMP4 antagonist Chordin, the Wnt and BMP antagonist Sizzled, and the Wnt, BMP and Nodal antagonist Cerberus. cerberus is an apparent ‘immediate-early’ target of Snail2 regulation…”
Background
A pan-cancer analysis of secreted Frizzled-related proteins: re-examining their proposed tumour suppressive function
Vincent, K. M., & Postovit, L.-M. M. (2017) · Scientific Reports · doi:10.1038/srep42719 · Cited by 51
Molecular mechanisms of cell-cell signaling by the Spemann-Mangold organizer
Robertis, E. M. D., Wessely, O., Oelgeschläger, M., Brizuela, B. J., Pera, E. M., Larraı́n, J., Abreu, J. G., & Bachiller, D. (2001) · The International Journal of Developmental Biology · doi:10.1387/ijdb.11291846 · Cited by 52
The ventralized ogon mutant phenotype is caused by a mutation in the zebrafish homologue of Sizzled, a secreted Frizzled-related protein
Martyn, U., & Schulte‐Merker, S. (2003) · Developmental Biology · doi:10.1016/s0012-1606(03)00221-5 · Cited by 51
Patterning and lineage specification in the amphibian embryo
Chan, A. P.-Y., & Etkin, L. D. (2001) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/s0070-2153(01)51001-7 · Cited by 51
Sfrp-1 and sfrp-2 are expressed in overlapping and distinct domains during chick development
Terry, K., Magan, H., Baranski, M., & Burrus, L. W. (2000) · Mechanisms of Development · doi:10.1016/s0925-4773(00)00407-x · Cited by 51
“…et al., 1998; Lescher et al., 1998; Leyns et al., 1997; Lin et al., 1997; Mayr et al., 1997; Melkonyan et al., 1997; Pfeffer et al., 1997; Rattner et al., 1997; Salic et al., 1997; Shirozu et al., 1996; Wada et al., 1999; Wang et al., 1997a,b; Wolf et al., 1997; Xu et al., 1998; Zhou et al., 1998).”
Crystal structure of R-spondin 2 in complex with the ectodomains of its receptors LGR5 and ZNRF3
Zebisch, M., & Jones, E. Y. (2015) · Journal of Structural Biology · doi:10.1016/j.jsb.2015.05.008 · Cited by 50
“…are secreted which function by blocking receptor access (Semenov et al., 2001; Mao et al., 2001; Semenov et al., 2005), sequestering Wnt ligands (Wang et al., 1997; Leyns et al., 1997; Malinauskas et al., 2011) or degrading Wnt (Zhang et al., 2012; Kakugawa et al., 2015; Zhang et al., 2015).”
Hex acts with β-catenin to regulate anteroposterior patterning via a Groucho-related co-repressor and Nodal
Zamparini, A. L., Watts, T., Gardner, C., Tomlinson, S. R., Johnston, G. I., & Brickman, J. M. (2006) · Development · doi:10.1242/dev.02516 · Cited by 51
Antimorphic goosecoids
Ferreiro, B., Artinger, M., Cho, K. W. Y., & Niehrs, C. (1998) · Development · doi:10.1242/dev.125.8.1347 · Cited by 50
A System-Wide Investigation of the Dynamics of Wnt Signaling Reveals Novel Phases of Transcriptional Regulation
Gujral, T. S., & MacBeath, G. (2010) · PLoS ONE · doi:10.1371/journal.pone.0010024 · Cited by 49
“signaling by binding directly to Wnt proteins and preventing their interactions with Frizzled receptors[26,27].”
Background
The Advantages and Disadvantages of Sfrp1 and Sfrp2 Expression in Pathological Events
Esteve, P., & Bovolenta, P. (2010) · The Tohoku Journal of Experimental Medicine · doi:10.1620/tjem.221.11 · Cited by 52
“Secreted Frizzled Related Proteins (Sfrps) were initially and independently identified as soluble factors implicated in early embryonic development (Leyns et al. 1997; Wang et al. 1997) and as modulators of apoptotic”
Background
Dickkopf1 and the Spemann-Mangold head organizer
Niehrs, C., Kazanskaya, O., Wu, W., & Glinka, A. (2001) · The International Journal of Developmental Biology · doi:10.1387/ijdb.11291852 · Cited by 49
“Similar to cerberus, the Wnt antagonist frzb is expressed in the Xenopus head organizer and is capable of inducing heads in conjunction with BMP inhibitors (Leyns et al., 1997; Wang et al., 1997; Glinka et al., 1998).”
A role for Xenopus Frizzled 8 in dorsal development
Itoh, K., Jacob, J., & Sokol, S. Y. (1998) · Mechanisms of Development · doi:10.1016/s0925-4773(98)00076-8 · Cited by 49
The frizzled motif: in how many different protein families does it occur?
Rehn, M., Pihlajaniemi, T. A., Hofmann, K., & Bücher, P. (1998) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(98)01290-0 · Cited by 49
Heparan Sulfate Proteoglycan Clustering in Wnt Signaling and Dispersal
Mii, Y., & Takada, S. (2020) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.00631 · Cited by 48
The complex roles of Wnt antagonists in RCC
Saini, S., Majid, S., & Dahiya, R. (2011) · Nature Reviews Urology · doi:10.1038/nrurol.2011.146 · Cited by 48
“The sFRP family sFRPs were initially identified as soluble factors implicated in early embryonic developmen”
Background
Early neural crest induction requires an initial inhibition of Wnt signals
Steventon, B., & Mayor, R. (2012) · Developmental Biology · doi:10.1016/j.ydbio.2012.02.029 · Cited by 48
“…al., 1996; De Robertis and Kuroda, 2004; Glinka et al., 1998; Leyns et al., 1997; Piccolo et al., 1999; Piccolo et al., 1999; Shibata et al., 2000; Wang et al., 1997) start to be expressed in the organiser, and concomitantly, Wnt8 starts to be downregulated in this territory as a consequence of…”
The thyroid hormone nuclear receptors and the Wnt/β-catenin pathway: An intriguing liaison
Skah, S., Uchuya-Castillo, J., Sirakov, M., & Plateroti, M. (2017) · Developmental Biology · doi:10.1016/j.ydbio.2017.01.003 · Cited by 47
A novel gene’s role in an ancient mechanism: secreted Frizzled-related protein 1 is a critical component in the anterior–posterior Wnt signaling network that governs the establishment of the anterior neuroectoderm in sea urchin embryos
Khadka, A., Martínez-Bartolomé, M., Burr, S. D., & Range, R. (2018) · EvoDevo · doi:10.1186/s13227-017-0089-3 · Cited by 47
Role of WNT signaling in normal and malignant hematopoiesis.
Khan, N. I., & Bendall, L. J. (2006) · PubMed · doi:10.14670/hh-21.761 · Cited by 47
Control of EGF receptor activation in Drosophila
Wasserman, J. D., & Freeman, M. (1997) · Trends in Cell Biology · doi:10.1016/s0962-8924(97)01143-4 · Cited by 47
Wnt pathway antagonists and angiogenesis
Zhang, B., & Ma, J. (2010) · Protein & Cell · doi:10.1007/s13238-010-0112-0 · Cited by 46
“In the study of Xenopus embryos development, sFRP3/Frzb was reported to interact with Xwnt8 and block the Xwnt-8 signaling (Leyns et al., 1997; Wang et al., 1997a).”
Background
Methylation and Loss of Secreted Frizzled-Related Protein 3 Enhances Melanoma Cell Migration and Invasion
Ekström, E. J., Sherwood, V., & Andersson, T. (2011) · PLoS ONE · doi:10.1371/journal.pone.0018674 · Cited by 46
“accordance with previous findings in other systems [38,39,40].”
Result
SFRP1 is required for the proper establishment of the eye field in the medaka fish
Esteve, P., López-Rı́os, J., & Bovolenta, P. (2004) · Mechanisms of Development · doi:10.1016/j.mod.2004.03.003 · Cited by 46
“In Xenopus, over-expression of different SFRP family members, including FrzB, FrzA, Sizzled and Sizzled2, leads to variable degrees of embryo dorso-anteriorisation, interpreted as an acquisition of dorsal fates at the expense of the ventro-lateral domains, due to their antagonism with Wnt8 marginal activity (Leyns et…”
Influential
Netrin UNC-6 and the Regulation of Branching and Extension of Motoneuron Axons from the Ventral Nerve Cord of Caenorhabditis elegans
Lim, Y.-S., Mallapur, S., Kao, G., Ren, X., & Wadsworth, W. G. (1999) · Journal of Neuroscience · doi:10.1523/jneurosci.19-16-07048.1999 · Cited by 46
“Domains VI and V are related to the N terminus of laminin subunits, whereas domain C is shared among netrins and is related to the C terminus of Frzb, an antagonist of the Wnt receptor (Leyns et al., 1997; Wang et al., 1997).”
Background
Evidence for a frizzled-mediated wnt pathway required for zebrafish dorsal mesoderm formation
Nasevicius, A., Hyatt, T. M., Kim, H., Guttman, J., Walsh, E., Sumanas, S., Wang, Y., & Ekker, S. C. (1998) · Development · doi:10.1242/dev.125.21.4283 · Cited by 46
Autonomous and nonautonomous regulation of axis formation by antagonistic signaling via 7-span cAMP receptors and GSK3 in DictyosteliumPreprint
Ginsburg, G. T., & Kimmel, A. R. (1997) · Genes & Development · doi:10.1101/gad.11.16.2112 · Cited by 46
“Wnt concentration, in turn, is sensitive to the secreted, Fz-related proteins Frizzled in bone (Frzb) and Frizzled-related protein (FRP) (Leyns et al. 1997; Wang et al. 1997; J. Rubin, X.”
Background
Specificity of WNT-receptor interactions
Hsieh, J.-C. (2004) · Frontiers in bioscience · doi:10.2741/1321 · Cited by 46
SFRP1 modulates retina cell differentiation through aβ-catenin-independent mechanism
Esteve, P., Trousse, F., Rodríguez, J., & Bovolenta, P. (2003) · Journal of Cell Science · doi:10.1242/jcs.00452 · Cited by 45
“…widespread expression, complementary in several locations to those of Wnts, have suggested that SFRPs could act as competitive antagonists of WNT-mediated β-catenin signalling, binding and preventing Wnt interaction with the Fz receptors (Leyns et al., 1997; Wang et al., 1997; Xu et al., 1998).”
Background
Formation and maintenance of the organizer among the vertebrates
Joubin, K., & Stern, C. D. (2001) · The International Journal of Developmental Biology · doi:10.1387/ijdb.11291844 · Cited by 45
“Additionally, a family of secreted Wnt inhibitors, called the Fzb’s, has been characterized (Leyns et al., 1997; Salic et al., 1997; Wang et al., 1997a; 1997b).”
Background
Serum Dickkopf-1 as a Biomarker for the Diagnosis of Hepatocellular Carcinoma
Kim, S. U., Park, J. H., Kim, H.-S., Lee, J. M., Lee, H. G., Kim, H., Choi, S. H., Baek, S., Kim, B. K., Park, J. Y., et al. (2015) · Yonsei Medical Journal · doi:10.3349/ymj.2015.56.5.1296 · Cited by 44
3 Molecular Regulation of Pronephric Development
Carroll, T. J., Wallingford, J. B., Seufert, D. W., & Vize, P. D. (1998) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/s0070-2153(08)60467-6 · Cited by 44
RGS proteins inhibit Xwnt-8 signaling in Xenopus embryonic development
Wu, C., Zeng, Q., Blumer, K., & Muslin, A. J. (2000) · Development · doi:10.1242/dev.127.13.2773 · Cited by 44
Evidence for a differential association of the Arg200Trp single-nucleotide polymorphism in FRZB with hip osteoarthritis and osteoporosis
Lories, R. J. U., Boonen, S., Peeters, J., de Vlam, K., & Luyten, F. P. (2005) · Lara D. Veeken · doi:10.1093/rheumatology/kei148 · Cited by 44
“The induction of autoantibodies in the treatment of ankylosing spondylitis with infliximab has also been described, but to our knowledge it has never been associated previously with induction of a lupus-like syndrome [4, 5].”
BackgroundSelf-citation
Secreted HHIP1 interacts with heparan sulfate and regulates Hedgehog ligand localization and function
Holtz, A. M., Griffiths, S. C., Davis, S. J., Bishop, B., Siebold, C., & Allen, B. L. (2015) · The Journal of Cell Biology · doi:10.1083/jcb.201411024 · Cited by 43
The APC tumor suppressor protein in development and cancer
Moon, R. T., & Miller, J. R. (1997) · Trends in Genetics · doi:10.1016/s0168-9525(97)01196-7 · Cited by 43
Suppression of GATA factor activity causes axis duplication in Xenopus
Sykes, T. G., Rodaway, A., Walmsley, M. E., & Patient, R. K. (1998) · Development · doi:10.1242/dev.125.23.4595 · Cited by 43
Completion of the epithelial to mesenchymal transition in zebrafish mesoderm requires Spadetail
Row, R. H., Maître, J., Martin, B. L., Stockinger, P., Heisenberg, C., & Kimelman, D. (2011) · Developmental Biology · doi:10.1016/j.ydbio.2011.03.025 · Cited by 42
“MBT acts to promote ventral/lateral mesoderm and to repress axial fates (Glinka et al., 1998; Hashimoto et al., 2000; Leyns et al., 1997; Ramel and Lekven, 2004; Salic et al., 1997; Wang et al., 1997).”
Transcriptome Analysis Reveals Differentially Expressed Transcripts in Rat Adrenal Zona Glomerulosa and Zona Fasciculata
Nishimoto, K., Rigsby, C. S., Wang, T., Mukai, K., Gómez-Sánchez, C. E., Rainey, W. E., & Seki, T. (2012) · Endocrinology · doi:10.1210/en.2011-1915 · Cited by 42
“The gene is initially identified through its sequence similarity with the Drosophila gene Frizzled (45) and shown to inhibit the Wnt signaling (46, 47).”
Background
Differential Expression of Rat Frizzled-Related frzb-1 and Frizzled Receptor fz1 and fz2 Genes in the Rat Aorta After Balloon Injury
Mao, C. D., Tahlil, O., Pueyo, M. E., Steg, P. G., & Soubrier, F. (2000) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.20.1.43 · Cited by 42
Joint homeostasis, restoration, and remodeling in osteoarthritis
Lories, R. J. U. (2008) · Best Practice & Research Clinical Rheumatology · doi:10.1016/j.berh.2007.12.001 · Cited by 41
Regulation of Secreted Frizzled-related Protein-1 by Heparin
Zhong, X., Desilva, T., Lin, L., Bodine, P. V. N., Bhat, R. A., Presman, E., Pocas, J. R., Stahl, M., & Kriz, R. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m609096200 · Cited by 43

Authors: Wang, S., Krinks, M., Lin, K., Luyten, F. P., & Moos, M.
Affiliations: United States Food and Drug Administration (US)
Center for Biologics Evaluation and Research (US)
National Institutes of Health (US)
Corresponding author: Moos, Malcolm
ORCID: Wang, Shouwen
Luyten, Frank P
Moos, Malcolm
Venue: Cell
Publisher: Elsevier BV
Published: 1997-03
Volume: 88
Issue: 6
Pages: 757-766
ISSN: 0092-8674 (ISSN-L)
DOI: 10.1016/s0092-8674(00)81922-4
OpenAlex: W1988350691
Cited by: 518
References: 70
Citations by year: 2026: 3 · 2025: 11 · 2024: 8 · 2023: 9 · 2022: 11 · 2021: 6 · 2020: 12 · 2019: 3 · 2018: 13 · 2017: 17 · 2016: 6 · 2015: 12 · 2014: 15 · 2013: 9 · 2012: 14
FWCI: 17.08
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/open-access/userlicense/1.0/ (vor, 5982 d)
Locations: Cell (publishedVersion)
PubMed (publishedVersion)
Funders: George Washington University
National Institutes of Health
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Health Sciences › Medicine › Oncology › Renal and related cancers
Keywords: FRZB, Spemann organizer, dorsalization, ventralization, gastrulation, axial patterning, MyoD, Wnt signaling inhibition, secreted proteins, Xenopus embryo
MeSH terms: Frizzled-Related Proteins, Animals, Biological Transport, Cell Communication, Dogs, Embryonic Induction, Female, Glycoproteins, Male, Mesoderm, Microsomes, Protein Binding, Proteins, Rabbits, RNA, Messenger, Solubility, Transcription, Genetic, Xenopus, Zygote, MyoD Protein, Gene Expression Regulation, Developmental, COS Cells, Zebrafish Proteins, Intracellular Signaling Peptides and Proteins, Wnt Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Frzb, a Secreted Protein Expressed in the Spemann Organizer, Binds and Inhibits Wnt-8

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks