CoChat
Sign Up
Cited by 128Axon Guidance and Neuronal SignalingDevelopmental Biology and Gene RegulationNeurogenesis and neuroplasticity mechanisms

A truncated Reelin protein is produced but not secreted in the ‘Orleans’ reeler mutation (Reln)

de Bergeyck, V., Nakajima, K., de Rouvroit, C. Lambert, Naerhuyzen, B., Goffinet, A.M., Miyata, T., Ogawa, M., Mikoshiba, K.·1997·Molecular Brain Research
DOI 10.1016/s0169-328x(97)00166-6
Open this paper in CoChat — free account

Abstract

Reelin is the protein defective in reeler mutant mice [I. Bar, C. Lambert de Rouvroit, I. Royaux, D.B. Krizman, C. Dernoncourt, D. Ruelle, M.C. Beckers, A.M. Goffinet, A YAC contig containing the reeler locus with preliminary characterization of candidate gene fragments, Genomics 26 (1995) 543-549; G. D'Arcangelo, G.G. Miao, S.C. Chen, H.D. Soares, J.I. Morgan, T. Curran, A protein related to extracellular matrix proteins deleted in the mouse mutant reeler, Nature 374 (1995) 719-723; S. Hirotsune, T. Takahara, N. Sasaki, K. Hirose, A. Yoshiki, T. Ohashi, M. Kusakabe, Y. Murakami, M. Muramatsu, S. Watanabe, K. Nakao, M. Katsuki, Y. Hayashizaki, The reeler gene encodes a protein with an EGF-like motif expressed by pioneer neurons, Nature Genet. 10 (1995) 77-83]. In the Orleans allele of reeler (symbol: Reln[rl-Orl]), a 220 nucleotide deletion is present in the 3' region of the Reelin message, resulting in a frame shift with production of a predicted protein amputated from its C-terminal amino acids. In this study, we first show that the predicted truncated protein indeed exists in Orleans reeler mice, using several anti-Reelin antibodies. Three antibodies are directed against epitopes located in the N-terminal region of the protein, namely: monoclonal antibody CR-50 [M. Ogawa, T. Miyata, K. Nakajima, K. Yagyu, M. Seike, K. Ikenaka, H. Yamamoto, K. Mikoshiba, The reeler gene-associated antigen on Cajal-Retzius neurons is a crucial molecule for laminar organization of cortical neurons, Neuron 14 (1995) 899-912] (epitope region between Reelin residues 251-407), monoclonal antibody G10 (epitope located between amino acids 199 and 244) and the polyclonal antipeptide rp4 (positions 381-399). A fourth antibody, antipeptide rp5, reacts with the C-terminal (3443-3461) Reelin sequence. In normal embryos, all four antibodies stained cells in the marginal zone with features of Cajal-Retzius cells. While N-terminal specific antibodies detected Reelin immunoreactivity in mouse embryos homozygous for the reeler-Orleans mutation, no staining was obtained with the rp5 antibody, showing the presence of a truncated protein. Moreover, although Reelin could be detected at the surface of living Cajal-Retzius cells of normal mice, it was not revealed after vital staining of embryonic cortex from Orleans reeler mice. These results indicate that the C-terminal region of Reelin is essential for its secretion and suggest that the Orleans reeler phenotype is due to defective Reelin secretion rather than to secretion of an inactive protein.

Integrity signals

Registry

Crossref · 10.1016/s0169-328x(97)00166-6

Cites

12 of 12 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
de Bergeyck, V., Nakajima, K., de Rouvroit, C. L., Naerhuyzen, B., Goffinet, A. M., Miyata, T., Ogawa, M., & Mikoshiba, K.
Affiliations
  • RIKEN (JP)
  • University of Namur (BE)
  • Kōchi University (JP)
Corresponding author
Nakajima, K.
ORCID
Venue
Molecular Brain Research
Publisher
Elsevier BV
Published
1997-10
Volume
50
Issue
1-2
Pages
85-90
ISSN
0169-328X (ISSN-L)
DOI
10.1016/s0169-328x(97)00166-6
OpenAlex
W2030718949
Cited by
128
References
12
Citations by year
2025: 3 · 2024: 3 · 2023: 1 · 2022: 3 · 2021: 2 · 2020: 7 · 2019: 2 · 2018: 4 · 2017: 7 · 2016: 6 · 2015: 5 · 2014: 4 · 2013: 3 · 2012: 5
FWCI
2.63
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Molecular Brain Research (publishedVersion)
  • PubMed (publishedVersion)
  • DIAL (Catholic University of Leuven) (submittedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Keywords
reelin, RELN gene, frame shift, protein secretion, N-terminal epitope, antibody specificity, Cajal-Retzius neurons, extracellular matrix proteins
MeSH terms
Reelin Protein, Amino Acid Sequence, Animals, Cerebral Cortex, Immunohistochemistry, Mice, Neurologic Mutants, Molecular Sequence Data, Mutation, Nerve Tissue Proteins, Serine Endopeptidases, Cell Adhesion Molecules, Neuronal, Extracellular Matrix Proteins, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review