The DOI matches its Crossref record; 63 of the 63 references it cites were found in a registry or an index.
Photoprotective potential of lycopene, β-carotene, vitamin E, vitamin C and carnosic acid in UVA-irradiated human skin fibroblasts
Abstract
The photoprotective potential of the dietary antioxidants vitamin C, vitamin E, lycopene, beta-carotene, and the rosemary polyphenol, carnosic acid, was tested in human dermal fibroblasts exposed to ultraviolet-A (UVA) light. The carotenoids were prepared in special nanoparticle formulations together with vitamin C and/or vitamin E. Nanoparticle formulations, in contrast to dimethylsulphoxide, stablized lycopene in the cell culture medium and allowed efficient cellular uptake. The presence of vitamin E in the formulation further increased the stability and cellular uptake of lycopene. UVA irradiation of the human skin fibroblasts led to a 10-15-fold rise in metalloproteinase 1 (MMP-1) mRNA. This rise was suppressed in the presence of low microM concentrations of vitamin E, vitamin C, or carnosic acid but not with beta-carotene or lycopene. Indeed, in the presence of 0.5-1.0 microM beta-carotene or lycopene, the UVA-induced MMP-1 mRNA was further increased by 1.5-2-fold. This increase was totally suppressed when vitamin E was included in the nanoparticle formulation. Heme-oxygenase 1 (HO-1) mRNA expression was strongly induced by UVA irradiation but none of the antioxidants inhibited this effect at the concentrations used in this study. Indeed, beta-carotene or lycopene (0.5-1.0 microM) led to a further 1.5-fold rise in the UVA-induced HO-1 mRNA levels. In conclusion, vitamin C, vitamin E, and carnosic acid showed photoprotective potential. Lycopene and beta-carotene did not protect on their own but in the presence of vitamin E, their stability in culture was improved and the rise in MMP-1 mRNA expression was suppressed, suggesting a requirement for antioxidant protection of the carotenoids against formation of oxidative derivatives that can influence the cellular and molecular responses.
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“Ultraviolet (UV) irradiation causes several areas of damage to the skin.Together with immune suppression, cancer, tanning, and sunburn, it provokes injuries called photoaging, consisting of connective tissue degradation [62] (Figure 4).”
From Licorice (Glycyrrhiza glabra, G. uralensis, and G. inflata) and Their Constituents as Active Cosmeceutical Ingredients (2022) · cites this paper as [62]
In the citing paper
“Offord et al. assessed the photoprotective potential of vitamin C and other antioxidants, confirming that vitamin C plays a significant role in protecting the skin against UVA radiation [62].”
From Bioactive Compounds for Skin Health: A Review (2021) · cites this paper as [62]
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Antioxidant and prooxidant properties of flavonoids
Procházková, D., Boušová, I., & Wilhelmová, N. (2011) · Fitoterapia · doi:10.1016/j.fitote.2011.01.018 · Cited by 1,572
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Carotenoids and Chlorophylls as Antioxidants
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Metadata
- Authors
- Offord, E. A., Gautier, J.-C., Avanti, O., Scaletta, C., Runge, F., Krämer, K., & Applegate, L. A.
- Affiliations
- Nestlé (Switzerland) (CH)
- Centre Hospitalier Universitaire Vaudois (CH)
- BASF (Germany) (DE)
- Corresponding author
- Offord, Elizabeth A.
- Venue
- Free Radical Biology and Medicine
- Publisher
- Elsevier BV
- Published
- 2002-06
- Volume
- 32
- Issue
- 12
- Pages
- 1293-1303
- ISSN
- 0891-5849 (ISSN-L)
- OpenAlex
- W2052373472
- Cited by
- 254
- References
- 63
- Citations by year
- 2026: 5 · 2025: 8 · 2024: 10 · 2023: 5 · 2022: 8 · 2021: 8 · 2020: 9 · 2019: 9 · 2018: 9 · 2017: 8 · 2016: 17 · 2015: 11 · 2014: 5 · 2013: 12 · 2012: 12
- FWCI
- 10.77
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Free Radical Biology and Medicine (publishedVersion)
- PubMed (publishedVersion)
- IRIS (submittedVersion)
- SERVAL (Université de Lausanne) (submittedVersion)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
- Keywords
- photoprotection, lycopene, beta-carotene, vitamin E, vitamin C, carnosic acid, UVA irradiation, human dermal fibroblasts, MMP-1, heme oxygenase-1, carotenoid stability, nanoparticle formulation, dietary antioxidants, oxidative stress
- MeSH terms
- Lycopene, Adult, Antioxidants, Ascorbic Acid, Carotenoids, Cells, Cultured, Chromatography, High Pressure Liquid, Diterpenes, DNA Damage, Fibroblasts, Heme Oxygenase (Decyclizing), Humans, Male, Membrane Proteins, Plant Extracts, Radiation-Protective Agents, Skin, Ultraviolet Rays, Vitamin E, Blotting, Northern, Biomarkers, beta Carotene, Cytoprotection, Matrix Metalloproteinase 1, Abietanes, Heme Oxygenase-1
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