CoChat
Sign Up
Cited by 254Skin Protection and AgingAntioxidant Activity and Oxidative Stressmelanin and skin pigmentation

The DOI matches its Crossref record; 63 of the 63 references it cites were found in a registry or an index.

Photoprotective potential of lycopene, β-carotene, vitamin E, vitamin C and carnosic acid in UVA-irradiated human skin fibroblasts

Offord, Elizabeth A., Gautier, Jean-Charles, Avanti, Ornella, Scaletta, Corinne, Runge, Frank, Krämer, Klaus, Applegate, Lee Ann·2002·Free Radical Biology and Medicine
DOI 10.1016/s0891-5849(02)00831-6
Open this paper in CoChat — free account

Abstract

The photoprotective potential of the dietary antioxidants vitamin C, vitamin E, lycopene, beta-carotene, and the rosemary polyphenol, carnosic acid, was tested in human dermal fibroblasts exposed to ultraviolet-A (UVA) light. The carotenoids were prepared in special nanoparticle formulations together with vitamin C and/or vitamin E. Nanoparticle formulations, in contrast to dimethylsulphoxide, stablized lycopene in the cell culture medium and allowed efficient cellular uptake. The presence of vitamin E in the formulation further increased the stability and cellular uptake of lycopene. UVA irradiation of the human skin fibroblasts led to a 10-15-fold rise in metalloproteinase 1 (MMP-1) mRNA. This rise was suppressed in the presence of low microM concentrations of vitamin E, vitamin C, or carnosic acid but not with beta-carotene or lycopene. Indeed, in the presence of 0.5-1.0 microM beta-carotene or lycopene, the UVA-induced MMP-1 mRNA was further increased by 1.5-2-fold. This increase was totally suppressed when vitamin E was included in the nanoparticle formulation. Heme-oxygenase 1 (HO-1) mRNA expression was strongly induced by UVA irradiation but none of the antioxidants inhibited this effect at the concentrations used in this study. Indeed, beta-carotene or lycopene (0.5-1.0 microM) led to a further 1.5-fold rise in the UVA-induced HO-1 mRNA levels. In conclusion, vitamin C, vitamin E, and carnosic acid showed photoprotective potential. Lycopene and beta-carotene did not protect on their own but in the presence of vitamin E, their stability in culture was improved and the rise in MMP-1 mRNA expression was suppressed, suggesting a requirement for antioxidant protection of the carotenoids against formation of oxidative derivatives that can influence the cellular and molecular responses.

Overview

Integrity signals

Registry

Crossref · 10.1016/s0891-5849(02)00831-6

Cites

63 of 63 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Research works printed but not linked: 2

  1. Basu-Modak, 1993, Singlet oxygen, Cancer Res., 53, 4505
  2. Aeschbach, 1993, Carnosic acid obtension and uses

Cited by

Sorted by citations

See all 254

Related

See all

Metadata

Authors
Offord, E. A., Gautier, J.-C., Avanti, O., Scaletta, C., Runge, F., Krämer, K., & Applegate, L. A.
Affiliations
  • Nestlé (Switzerland) (CH)
  • Centre Hospitalier Universitaire Vaudois (CH)
  • BASF (Germany) (DE)
Corresponding author
Offord, Elizabeth A.
ORCID
Venue
Free Radical Biology and Medicine
Publisher
Elsevier BV
Published
2002-06
Volume
32
Issue
12
Pages
1293-1303
ISSN
0891-5849 (ISSN-L)
DOI
10.1016/s0891-5849(02)00831-6
OpenAlex
W2052373472
Cited by
254
References
63
Citations by year
2026: 5 · 2025: 8 · 2024: 10 · 2023: 5 · 2022: 8 · 2021: 8 · 2020: 9 · 2019: 9 · 2018: 9 · 2017: 8 · 2016: 17 · 2015: 11 · 2014: 5 · 2013: 12 · 2012: 12
FWCI
10.77
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Free Radical Biology and Medicine (publishedVersion)
  • PubMed (publishedVersion)
  • IRIS (submittedVersion)
  • SERVAL (Université de Lausanne) (submittedVersion)
Topics
  • Health Sciences › Medicine › Dermatology › Skin Protection and Aging
  • Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
Keywords
photoprotection, lycopene, beta-carotene, vitamin E, vitamin C, carnosic acid, UVA irradiation, human dermal fibroblasts, MMP-1, heme oxygenase-1, carotenoid stability, nanoparticle formulation, dietary antioxidants, oxidative stress
MeSH terms
Lycopene, Adult, Antioxidants, Ascorbic Acid, Carotenoids, Cells, Cultured, Chromatography, High Pressure Liquid, Diterpenes, DNA Damage, Fibroblasts, Heme Oxygenase (Decyclizing), Humans, Male, Membrane Proteins, Plant Extracts, Radiation-Protective Agents, Skin, Ultraviolet Rays, Vitamin E, Blotting, Northern, Biomarkers, beta Carotene, Cytoprotection, Matrix Metalloproteinase 1, Abietanes, Heme Oxygenase-1

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review