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Cited by 52Skin Protection and Agingmelanin and skin pigmentationProtease and Inhibitor Mechanisms

β-Carotene Inhibits UVA-Induced Matrix Metalloprotease 1 and 10 Expression in Keratinocytes by a Singlet Oxygen-Dependent Mechanism

Wertz, Karin, Seifert, Nicole, Hunziker, Petra Buchwald, Riss, Georges, Wyss, Adrian, Lankin, Christopher, Goralczyk, Regina·2004·Free Radical Biology and Medicine
DOI 10.1016/j.freeradbiomed.2004.05.018
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Abstract

UVA exposure causes skin photoaging by singlet oxygen (1)O(2)-mediated induction of, e.g., matrix metalloproteases (MMPs). We assessed whether pretreatment with beta-carotene, a (1)O(2) quencher and retinoic acid (RA) precursor, interferes with UVA-induced gene regulation. HaCaT keratinocytes were precultured with beta-carotene at physiological concentrations (0.5, 1.5, and 3.0 microM) prior to exposure to UVA from a Hönle solar simulator (270 kJ/m(2)). HaCaT cells accumulated beta-carotene in a time- and dose-dependent manner. UVA irradiation massively reduced the cellular beta-carotene content. Beta-carotene suppressed UVA-induction of MMP-1, MMP-3, and MMP-10, three major matrix metalloproteases involved in photoaging. We show that regulation by not only MMP-1, but also MMP-10, involves (1)O(2)-dependent mechanisms. Beta-carotene dose-dependently quenched (1)O(2)-mediated induction of MMP-1 and MMP-10. Thus, as in chemical solvent systems, beta-carotene quenches (1)O(2) also in living cells. Vitamin E did not cooperate with beta-carotene to further inhibit MMP induction. HaCaT cells produced weak retinoid activity from beta-carotene, as demonstrated by mild upregulation of RAR beta and activation of an RARE-dependent reporter gene. Beta-carotene did not regulate the genes encoding other RARs, RXRs, or the two beta-carotene cleavage enzymes. These results demonstrate that beta-carotene acts photoprotectively, and that this effect is mediated by (1)O(2) quenching.

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Crossref · 10.1016/j.freeradbiomed.2004.05.018

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118 of 118 references verified0 citation errors · 0 not checkable

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  • β-carotene is the precursor of retinoic acid.

    “β-carotene is the precursor of retinoic acid [154,155].”

    From The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence (2022) · cites this paper as [154]

  • In vitro, keratinocytes treatment with β-carotene, prior to UVA exposure, prevents the upregulation of MMP-1, MMP-3 and MMP-10, suggesting a protective role of β-carotene against OS-induced senescence.

    “In vitro, keratinocytes treatment with β-carotene, prior to UVA exposure, prevents the upregulation of MMP-1, MMP-3 and MMP-10, therefore suggesting a protective role of β-carotene against OS-induced senescence [154].”

    From The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence (2022) · cites this paper as [154]

  • β-carotene suppresses the UVA induction of MMP-1, MMP-3 and MMP-10.

    “This compound was found to suppress the UVA induction of MMP-1, MMP-3 and MMP-10, the three main metalloproteinases involved in photoaging.”

    From Bioactive Compounds for Skin Health: A Review (2021) · cites this paper as [228]

  • Carotenoids in the diet and in the form of dietary supplements have photoprotective effects.

    “Research confirms the photoprotective effects of carotenoids in the diet and in the form of dietary supplements.”

    From Bioactive Compounds for Skin Health: A Review (2021) · cites this paper as [228]

  • β-carotene blocks the 1O2-mediated induction of MMP-1 and MMP-10 in a dose-dependent manner.

    “Moreover, β-carotene was shown to block the 1O2-mediated induction of MMP-1 and MMP-10 in a dose-dependent manner [228].”

    From Bioactive Compounds for Skin Health: A Review (2021) · cites this paper as [228]

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Authors
Wertz, K., Seifert, N., Hunziker, P. B., Riss, G., Wyss, A., Lankin, C., & Goralczyk, R.
Venue
Free Radical Biology and Medicine
Publisher
Elsevier BV
Published
2004-09
Volume
37
Issue
5
Pages
654-670
ISSN
0891-5849 (ISSN-L)
DOI
10.1016/j.freeradbiomed.2004.05.018
OpenAlex
W2000001611
Cited by
52
References
119
Citations by year
2026: 2 · 2025: 2 · 2024: 4 · 2022: 3 · 2021: 3 · 2020: 2 · 2019: 2 · 2018: 2 · 2017: 3 · 2016: 3 · 2015: 2 · 2014: 4 · 2013: 2 · 2012: 1
FWCI
1.65
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Free Radical Biology and Medicine (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Dermatology › Skin Protection and Aging
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
Keywords
beta-carotene, UVA, photoaging, singlet oxygen, singlet oxygen quenching, MMP-1, matrix metalloproteinase-10, matrix metalloproteinase-3, keratinocytes, retinoic acid, retinoid X receptor, photoprotection, vitamin E
MeSH terms
Analysis of Variance, Antioxidants, Base Sequence, Biological Transport, Cell Line, Epidermis, Humans, Metalloendopeptidases, RNA, Sunlight, Ultraviolet Rays, Keratinocytes, DNA Primers, beta Carotene, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, Matrix Metalloproteinase 1, Singlet Oxygen, Matrix Metalloproteinase 10

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