Restricted expression and retinoic acid-induced downregulation of the retinaldehyde dehydrogenase type 2 (RALDH-2) gene during mouse development
Open accessAbstract
Retinaldehyde dehydrogenase type 2 (RALDH-2) was identified as a major retinoic acid generating enzyme in the early embryo. Here we report the expression domains of the RALDH-2 gene during mouse embryogenesis, which are likely to indicate regions of endogenous retinoic acid (RA) synthesis. During early gastrulation, RALDH-2 is expressed in the mesoderm adjacent to the node and primitive streak. At the headfold stage, mesodermal expression is restricted to posterior regions up to the base of the headfolds. Later, RALDH-2 is transiently expressed in the undifferentiated somites and the optic vesicles, and more persistently along the lateral walls of the intraembryonic coelom and around the hindgut diverticulum. The RALDH-2 expression domains in differentiating limbs, which include presumptive interdigital regions, coincide with, but slightly precede, those of the RA-inducible RAR beta gene. The RALDH-2 gene is also expressed in specific regions of the developing head, including the tooth buds, inner ear, meninges and pituitary gland, and in several viscera. Administration of a teratogenic dose of RA at embryonic day 8.5 results in downregulation of RALDH-2 transcript levels in caudal regions of the embryo, and may reflect a mechanism of negative feedback regulation of RA synthesis.
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The timepoint of Renshaw cell generation correlates with expression of the RA-synthesising enzyme Aldh1a2 in the adjacent somites.
“Retinoic acid (RA) promotes Renshaw cell identity in V1 neurons (Hoang et al., 2018), and the timepoint of their generation correlates with expression of the RA-synthesising enzyme Aldh1a2 in the adjacent somites (Niederreither et al., 1997).”
From Single cell transcriptomics reveals spatial and temporal dynamics of gene expression in the developing mouse spinal cord (2019) · cites this paper as Niederreither et al., 1997
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- Authors
- Niederreither, K., McCaffery, P., Dräger, U. C., Chambon, P., & Dollé, P.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Harvard University (US)
- Corresponding author
- Chambon, Pierre
- Venue
- Mechanisms of Development
- Publisher
- Elsevier BV
- Published
- 1997-02
- Volume
- 62
- Issue
- 1
- Pages
- 67-78
- ISSN
- 0925-4773 (ISSN-L)
- OpenAlex
- W2086345206
- Cited by
- 523
- References
- 62
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 7 · 2023: 9 · 2022: 14 · 2021: 17 · 2020: 16 · 2019: 14 · 2018: 15 · 2017: 11 · 2016: 12 · 2015: 12 · 2014: 17 · 2013: 15 · 2012: 16
- FWCI
- 7.70
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Mechanisms of Development (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Bristol-Myers Squibb
- Association pour la Recherche sur le Cancer
- Centre National de la Recherche Scientifique
- National Institutes of Health
- National Eye Institute (R01EY001938, EY-01938)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (P01HD005515, HD05515)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinoids in leukemia and cellular processes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Keywords
- retinoic acid synthesis, retinoic acid, mouse embryo development, gene expression domains, mesoderm, optic vesicle, somites, limb development, RARβ, negative feedback regulation
- MeSH terms
- Aldehyde Oxidoreductases, Animals, Female, Embryonic and Fetal Development, Pregnancy, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Developmental, Retinal Dehydrogenase, Mice
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