Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 227

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Phosphatidylinositol-4,5-bisphosphate is required for endocytic coated vesicle formation

Jost, Matthias, Simpson, Fiona, Kavran, Jennifer M., Lemmon, Mark A., Schmid, Sandra L.·1998·Current Biology
DOI 10.1016/s0960-9822(98)00022-0
Open this paper in CoChat — free account

Abstract

Receptor-mediated endocytosis via clathrin-coated vesicles has been extensively studied and, while many of the protein players have been identified, much remains unknown about the regulation of coat assembly and the mechanisms that drive vesicle formation [1]. Some components of the endocytic machinery interact with inositol polyphosphates and inositol lipids in vitro, implying a role for phosphatidylinositols in vivo [2] [3]. Specifically, the adaptor protein complex AP2 binds phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2), PtdIns(3)P, PtdIns(3,4,5)P3 and inositol phosphates. Phosphatidylinositol binding regulates AP2 self-assembly and the interactions of AP2 complexes with clathrin and with peptides containing endocytic motifs [4] [5]. The GTPase dynamin contains a pleckstrin homology (PH) domain that binds PtdIns(4,5)P2 and PtdIns(3,4,5)P3 to regulate GTPase activity in vitro [6] [7]. However, no direct evidence for the involvement of phosphatidylinositols in clathrin-mediated endocytosis exists to date. Using well-characterized PH domains as high affinity and high specificity probes in combination with a perforated cell assay that reconstitutes coated vesicle formation, we provide the first direct evidence that PtdIns(4,5)P2 is required for both early and late events in endocytic coated vesicle formation.

Overview

Integrity signals

Registry

Crossref · 10.1016/s0960-9822(98)00022-0

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Show all 1 references

Research works printed but not linked: 24

  1. Schmid, 1997, Clathrin-coated vesicle formation and protein sorting: an integrated process, Ann Rev Biochem, 66, 511, 10.1146/annurev.biochem.66.1.511 · DOI · Paper page
  2. Liscovitch, 1995, Signal transduction and membrane traffic: the PITP/phosphoinositide connection, Cell, 81, 659, 10.1016/0092-8674(95)90525-1 · DOI · Paper page
  3. De Camilli, 1996, Phosphoinositides as regulators in membrane traffic, Science, 271, 1533, 10.1126/science.271.5255.1533 · DOI · Paper page
  4. Gaidarov, 1996, A functional phosphatidylinositol 3,4,5-trisphosphate/phosphoinositide binding domain in the clathrin adaptor AP-2 alpha subunit. Implications for the endocytic pathway, J Biol Chem, 271, 20922, 10.1074/jbc.271.34.20922 · DOI
  5. Rapoport, 1997, Regulatory interactions in the recognition of endocytic sorting signals by AP-2 complexes, EMBO J, 16, 2240, 10.1093/emboj/16.9.2240 · DOI
  6. Barylko, 1998, Synergistic activation of dynamin GTPase by Grb2 and phosphoinositides, J Biol Chem, 273, 3791, 10.1074/jbc.273.6.3791 · DOI
  7. Klein, 1998, The PH-domains of dynamin isoforms require oligomerization for high-affinity phosphoinositide binding, J Biol Chem, 273, 27725, 10.1074/jbc.273.42.27725 · DOI
  8. Schmid, 1991, Stage-specific assays for coated pit formation and coated vesicle budding in vitro, J Cell Biol, 114, 869, 10.1083/jcb.114.5.869 · DOI
  9. Smythe, 1992, Cytosol- and clathrin-dependent stimulation of endocytosis vitro by purified adaptors, J Cell Biol, 119, 1163, 10.1083/jcb.119.5.1163 · DOI
  10. Lodhi, 1979, Interactions of neomycin with monomolecular films of polyphosphoinositides and other lipids, Biochim Biophys Acta, 557, 1, 10.1016/0005-2736(79)90084-1 · DOI
  11. West, 1997, The role of ADP-ribosylation factor and phospholipase D in adaptor recruitment, J Cell Biol, 138, 1239, 10.1083/jcb.138.6.1239 · DOI
  12. Shaw, 1996, The pleckstrin homology domain: an intriguing multifunctional protein module, Bioessays, 18, 35, 10.1002/bies.950180109 · DOI · Paper page
  13. Lemmon, 1996, PH domains: diverse sequences with a common fold recruit signaling molecules to the cell surface, Cell, 85, 621, 10.1016/S0092-8674(00)81022-3 · DOI
  14. Rameh, 1997, A comparative analysis of the phosphoinositide binding specificity of pleckstrin homology domains, J Biol Chem, 272, 22059, 10.1074/jbc.272.35.22059 · DOI · Paper page
  15. Kavran, 1998, Specificity and promiscuity in phosphoinositide binding by pleckstrin homology (PH) domains, J Biol Chem, 273, 30497, 10.1074/jbc.273.46.30497 · DOI · Paper page
  16. Klarlund, 1997, Signaling by phosphoinositide-3,4,5-trisphosphate through proteins containing pleckstrin and Sec7 homology domains, Science, 275, 1927, 10.1126/science.275.5308.1927 · DOI · Paper page
  17. Hudson, 1997, Interaction of coatomer with aminoglycoside antibiotics: evidence that coatomer has at least two dilysine binding sites, Mol Biol Cell, 8, 1901, 10.1091/mbc.8.10.1901 · DOI
  18. Prentki, 1986, Neomycin: a specific drug to study the inositol-phospholipid signalling system?, FEBS Lett, 197, 285, 10.1016/0014-5793(86)80343-X · DOI
  19. Ferguson, 1995, Structure of the high affinity complex of inositol trisphosphate with a phospholipase C pleckstrin homology domain, Cell, 83, 1037, 10.1016/0092-8674(95)90219-8 · DOI · Paper page
  20. Mahaffey, 1990, Clathrin-coated pits contain an integral membrane protein that binds the AP-2 subunit with high affinity, J Biol Chem, 265, 16514, 10.1016/S0021-9258(17)46253-3 · DOI
  21. Salim, 1996, Distinct specificity in the recognition of phosphoinositides by the pleckstrin homology domains of dynamin and Bruton's tyrosine kinase, EMBO J, 15, 6241, 10.1002/j.1460-2075.1996.tb01014.x · DOI · Paper page
  22. M Achiriloaie, B Barylko, JP Albanesi, Essential role of the dynamin PH domain in receptor-mediated endocytosis, Mol Cell Biol in press.
  23. Carter, 1993, Multiple GTP-binding proteins participate in clathrin-coated vesicle-mediated endocytosis, J Cell Biol, 120, 37, 10.1083/jcb.120.1.37 · DOI
  24. Spiro, 1996, Wortmannin alters the transferrin receptor endocytic pathway vivo and in vitro, Mol Biol Cell, 7, 355, 10.1091/mbc.7.3.355 · DOI

Cited by

Metadata

Authors
Jost, M., Simpson, F., Kavran, J. M., Lemmon, M. A., & Schmid, S. L.
Venue
Current Biology
Publisher
Elsevier BV
Published
1998-12
Volume
8
Issue
25
Pages
1399-1404
ISSN
0960-9822
DOI
10.1016/s0960-9822(98)00022-0
Cited by
227
References
1
Language
en
Licence

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.