The DOI matches its Crossref record; 10 of the 10 references it cites were found in a registry or an index.
Matrix effect in bio-analysis of illicit drugs with LC-MS/MS: Influence of ionization type, sample preparation, and biofluid
Open accessAbstract
The purpose of the present work was to evaluate the synergistic effect of ionization type, sample preparation technique, and bio-fluid on the presence of matrix effect in quantitative liquid chromatography (LC)-MS/MS analysis of illicit drugs by post-column infusion experiments with morphine (10-microg/mL solution). Three bio-fluids (urine, oral fluid, and plasma) were pretreated with four sample preparation procedures [direct injection, dilution, protein precipitation, solid-phase extraction (SPE)] and analyzed by both LC-electrospray ionization (ESI)-MS/MS and LC-atmospheric pressure chemical ionization (APCI)-MS/MS. Our results indicated that both ionization types showed matrix effect, but ESI was more susceptible than APCI. Sample preparation could reduce (clean up) or magnify (pre-concentrate) matrix effect. Residual matrix components were specific to each bio-fluid and interfered at different time points in the chromatogram. We evaluated matrix effect in an early stage of method development and combined optimal ionization type and sample preparation technique for each bio-fluid. Simple dilution of urine was sufficient to allow for the analysis of the analytes of interest by LC-APCI-MS/MS. Acetonitrile protein precipitation provided both sample clean up and concentration for oral fluid analysis, while SPE was necessary for extensive clean up of plasma prior to LC-APCI-MS/MS.
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Cites
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Cited by
Taylor, P. J. (2005) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2004.11.007 · Cited by 1,248
Oral fluid testing for drugs of abuse
Bosker, W. M., & Huestis, M. A. (2009) · Clinical Chemistry · doi:10.1373/clinchem.2008.108670 · Cited by 378
Self-citationSigmund, G., Koch, A., Orlovius, A., Guddat, S., Thomas, A., Schänzer, W., & Thevis, M. (2014) · Drug Testing and Analysis · doi:10.1002/dta.1680 · Cited by 22
Metadata
- Authors
- Dams, R., Huestis, M. A., Lambert, W. E., & Murphy, C. M.
- Venue
- Journal of the American Society for Mass Spectrometry
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-11-01
- Volume
- 14
- Issue
- 11
- Pages
- 1290-1294
- ISSN
- 1044-0305 (ISSN-L)
- OpenAlex
- W2007455664
- Cited by
- 600
- References
- 10
- FWCI
- 23.63
- Type
- journal article
- Language
- en
- Open access
- green
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- Dams, R., Huestis, M. A., Lambert, W. E., & Murphy, C. M. (2003). Matrix effect in bio-analysis of illicit drugs with LC-MS/MS: Influence of ionization type, sample preparation, and biofluid. Journal of the American Society for Mass Spectrometry. https://doi.org/10.1016/s1044-0305(03)00574-9
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