The DOI matches its Crossref record; 40 of the 41 references it cites were found in a registry or an index.
Phosphorylation of the Stat1 Transactivation Domain Is Required for Full-Fledged IFN-γ-Dependent Innate Immunity
Open accessAbstract
Stat1 is phosphorylated on serine 727 within its transactivating domain (TAD) in response to interferons or other immunological signals. We generated gene-targeted mutant mice expressing a serine727-alanine mutant of Stat1. These animals showed increased mortality upon infection with Listeria monocytogenes and impaired clearance of the bacteria from spleen and liver. The Stat1S727A mice were more resistant to the LPS-induced septic shock syndrome, suggesting that Stat1 serine phosphorylation promotes inflammatory responses. Expression of IFN-gamma-induced genes was strongly reduced in macrophages expressing Stat1(S727A). While mutation of Stat1 at S727 did not reduce its binding to chromatin, association with the coactivator CBP and histone acetylation at the interferon-responsive GBP promoter was strongly reduced, suggesting defective recruitment of histone acetylases as the mechanism underlying IFN-gamma hyporesponsiveness. Our data demonstrate that the increase in transcription factor activity caused by Stat serine phosphorylation contributes to macrophage activation and to IFN-gamma-dependent immune responses in vivo.
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In the citing paper
“Blockage of STAT1 serine phosphorylation, using STAT1 serine mutants, significantly altered the IFN gamma transcriptional response and its ability to clear Listeria monocytogenes infection”
From CDK8 fine-tunes IL-6 transcriptional activities by limiting STAT3 resident time at the gene loci (2020) · cites this paper as Varinou et al., 2003
In the citing paper
“STAT1 Ser727Ala Knock-in mice elicited a poor immune response against Listeria monocytogenes infection (Varinou et al., 2003).”
From CDK8 fine-tunes IL-6 transcriptional activities by limiting STAT3 resident time at the gene loci (2020) · cites this paper as Varinou et al., 2003
Citations
Cites
STATs: transcriptional control and biological impact
Levy, D. E., & Darnell, J. E. (2002) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm909 · Cited by 3,201
Wen, Z., Zhong, Z., & Darnell, J. E. (1995) · Cell · doi:10.1016/0092-8674(95)90311-9 · Cited by 1,974
Meraz, M. A., White, J. M., Sheehan, K. C. F., Bach, E. A., Rodig, S. J., Dighe, A. S., Kaplan, D. H., Riley, J. K., Greenlund, A. C., Campbell, D., et al. (1996) · Cell · doi:10.1016/s0092-8674(00)81288-x · Cited by 1,656
Targeted Disruption of the Mouse Stat1 Gene Results in Compromised Innate Immunity to Viral Disease
Durbin, J. E., Hackenmiller, R., Simon, M. C., & Levy, D. E. (1996) · Cell · doi:10.1016/s0092-8674(00)81289-1 · Cited by 1,539
MacMicking, J. D., Nathan, C., Hom, G. J., Chartrain, N. A., Fletcher, D. S., Trumbauer, M. E., Stevens, K. A., Xie, Q. W., Sokol, K. A., Hutchinson, N. I., et al. (1995) · Cell · doi:10.1016/0092-8674(95)90085-3 · Cited by 1,445
Schwenk, F., Baron, U., & Rajewsky, K. (1995) · Nucleic Acids Research · doi:10.1093/nar/23.24.5080 · Cited by 1,289
Serine phosphorylation of STATs
Decker, T., & Kovarik, P. (2000) · Oncogene · doi:10.1038/sj.onc.1203481 · Cited by 887
Self-citationEssential function of histone deacetylase 1 in proliferation control and CDK inhibitor repression
Lagger, G., O'Carroll, D., Rembold, M., Khier, H., Tischler, J., Weitzer, G., Schuettengruber, B., Hauser, C., Brunmeir, R., Jenuwein, T., et al. (2002) · The EMBO Journal · doi:10.1093/emboj/21.11.2672 · Cited by 760
Defective TNF-α-Induced Apoptosis in STAT1-Null Cells Due to Low Constitutive Levels of Caspases
Kumar, A., Commane, M., Flickinger, T. W., Horvath, C. M., & Stark, G. R. (1997) · Science · doi:10.1126/science.278.5343.1630 · Cited by 508
Two contact regions between Stat1 and CBP/p300 in interferon γ signaling
Zhang, J. J., Vinkemeier, U., Gu, W., Chakravarti, D., Horvath, C. M., & Darnell, J. E., Jr. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.26.15092 · Cited by 487
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Items 101–102 of 102
NK Cell–Specific CDK8 Deletion Enhances Antitumor Responses
Witalisz-Siepracka, A., Gotthardt, D., Prchal-Murphy, M., Didara, Z., Menzl, I., Prinz, D., Edlinger, L., Putz, E. M., & Sexl, V. (2018) · Cancer Immunology Research · doi:10.1158/2326-6066.cir-17-0183 · Cited by 47
CDK8 fine-tunes IL-6 transcriptional activities by limiting STAT3 resident time at the gene loci
Martinez-Fabregas, J., Wang, L., Pohler, E., Cozzani, A., Kazemian, M., Mitra, S., & Moraga, I. (2020) · doi:10.1101/2020.03.19.998351 · Cited by 2
Metadata
- Authors
- Varinou, L., Ramsauer, K., Karaghiosoff, M., Kolbe, T., Pfeffer, K., Müller, M., & Decker, T.
- Affiliations
- University of Vienna (AT)
- Heinrich Heine University Düsseldorf (DE)
- Corresponding author
- Decker, Thomas
- Venue
- Immunity
- Publisher
- Elsevier BV
- Published
- 2003-12
- Volume
- 19
- Issue
- 6
- Pages
- 793-802
- ISSN
- 1074-7613 (ISSN-L)
- OpenAlex
- W2087814657
- Cited by
- 284
- References
- 41
- Citations by year
- 2026: 5 · 2025: 10 · 2024: 26 · 2023: 15 · 2022: 9 · 2021: 12 · 2020: 14 · 2019: 6 · 2018: 12 · 2017: 10 · 2016: 8 · 2015: 7 · 2014: 16 · 2013: 20 · 2012: 10
- FWCI
- 6.61
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Immunity (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Cytokine Signaling Pathways and Interactions
- Life Sciences › Immunology and Microbiology › Immunology › interferon and immune responses
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- STAT1, serine 727 phosphorylation, transactivation domain, interferon-gamma, innate immunity, Listeria monocytogenes infection, septic shock, macrophage activation, CBP, histone acetylation, transcriptional coactivator
- MeSH terms
- Animals, DNA-Binding Proteins, Immunity, Innate, Interferon-gamma, Lipopolysaccharides, Mice, Transgenic, Phosphorylation, Phosphotransferases, Serine, Shock, Septic, Trans-Activators, Amino Acid Substitution, STAT1 Transcription Factor, Mice
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