The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Monitoring Protein−Protein Interactions Using Split Synthetic Renilla Luciferase Protein-Fragment-Assisted Complementation
Open accessAbstract
In this study we developed an inducible synthetic renilla luciferase protein-fragment-assisted complementation-based bioluminescence assay to quantitatively measure real time protein-protein interactions in mammalian cells. We identified suitable sites to generate fragments of N and C portions of the protein that yield significant recovered activity through complementation. We validate complementation-based activation of split synthetic renilla luciferase protein driven by the interaction of two strongly interacting proteins, MyoD and Id, in five different cell lines utilizing transient transfection studies. The expression level of the system was also modulated by tumor necrosis factor alpha through NFkappaB-promoter/enhancer elements used to drive expression of the N portion of synthetic renilla luciferase reporter gene. This new system should help in studying protein-protein interactions and when used with other split reporters (e.g., split firefly luciferase) should help to monitor different components of an intracellular network.
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Cites
A novel genetic system to detect protein–protein interactions
Fields, S., & Song, O.-K. (1989) · Nature · doi:10.1038/340245a0 · Cited by 6,114
Split ubiquitin as a sensor of protein interactions in vivo.
Johnsson, N., & Varshavsky, A. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.22.10340 · Cited by 789
Optical imaging of Renilla luciferase reporter gene expression in living mice
Bhaumik, S., & Gambhir, S. S. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.012611099 · Cited by 536
Self-citationIsolation of an AP-1 Repressor by a Novel Method for Detecting Protein-Protein Interactions
Aronheim, A., Zandi, E., Hennemann, H., Elledge, S. J., & Karin, M. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.6.3094 · Cited by 462
Protein Splicing and Related Forms of Protein Autoprocessing
Paulus, H. (2000) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.69.1.447 · Cited by 425
Galarneau, A., Primeau, M., Trudeau, L.-E., & Michnick, S. W. (2002) · Nature Biotechnology · doi:10.1038/nbt0602-619 · Cited by 410
Pelletier, J. N., Campbell-Valois, F.-X., & Michnick, S. W. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.21.12141 · Cited by 382
Protein trans -splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803
Wu, H., Hu, Z., & Liu, X.-Q. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.16.9226 · Cited by 373
Isolation and expression of a cDNA encoding Renilla reniformis luciferase.
Lorenz, W. W., McCann, R. O., Longiaru, M., & Cormier, M. J. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.10.4438 · Cited by 366
Paulmurugan, R., Umezawa, Y., & Gambhir, S. S. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.242594299 · Cited by 334
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Cited by
Kerppola, T. K. (2008) · Annual Review of Biophysics · doi:10.1146/annurev.biophys.37.032807.125842 · Cited by 709
RETRACTED: Acriflavine inhibits HIF-1 dimerization, tumor growth, and vascularization
Lee, K., Zhang, H., Qian, D. Z., Rey, S., Liu, J. O., & Semenza, G. L. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0909353106 · Cited by 451
RetractedVarnum, M. M., Clayton, K. A., Yoshii-Kitahara, A., Yonemoto, G., Koro, L., Ikezu, S., & Ikezu, T. (2017) · Journal of Biological Chemistry · doi:10.1074/jbc.m116.759159 · Cited by 22
Metadata
- Authors
- Paulmurugan, R., & Gambhir, S. S.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-03-05
- Volume
- 75
- Issue
- 7
- Pages
- 1584-1589
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2009777487
- Cited by
- 310
- References
- 21
- FWCI
- 4.93
- Type
- journal article
- Language
- en
- Open access
- green
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