The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Microfluidic Device for Single-Cell Analysis
Abstract
We have developed a novel microfluidic device constructed from poly(dimethylsiloxane) using multilayer soft lithography technology for the analysis of single cells. The microfluidic network enables the passive and gentle separation of a single cell from the bulk cell suspension, and integrated valves and pumps enable the precise delivery of nanoliter volumes of reagents to that cell. Various applications are demonstrated, including cell viability assays, ionophore-mediated intracellular Ca2+ flux measurements, and multistep receptor-mediated Ca2+ measurements. These assays, and others, are achieved with significant improvements in reagent consumption, analysis time, and temporal resolution over macroscale alternatives.
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Citations
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Cited by
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Metadata
- Authors
- Wheeler, A. R., Throndset, W. R., Whelan, R. J., Leach, A. M., Zare, R. N., Liao, Y. H., Farrell, K., Manger, I. D., & Daridon, A.
- Venue
- Analytical Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-05-14
- Volume
- 75
- Issue
- 14
- Pages
- 3581-3586
- ISSN
- 0003-2700 (ISSN-L)
- 1520-6882
- OpenAlex
- W2036758684
- Cited by
- 600
- References
- 25
- FWCI
- 27.16
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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