Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 405

The DOI matches its Crossref record.

Microchip Device for Cell Lysis, Multiplex PCR Amplification, and Electrophoretic Sizing

Waters, Larry C., Jacobson, Stephen C., Kroutchinina, Natalia, Khandurina, Julia, Foote, Robert S., Ramsey, J. Michael·1998·Analytical Chemistry
DOI 10.1021/ac970642d
Open this paper in CoChat — free account

Abstract

The steps of cell lysis, multiplex PCR amplification, and electrophoretic analysis are executed sequentially on a monolithic microchip device. The entire microchip is thermally cycled to lyse cells and to amplify DNA, and the products are then analyzed using a sieving medium for size separation and an intercalating dye for fluorescence detection. Using a standard PCR protocol, a 500-base pair (bp) region of bacteriophage lambda DNA and 154-, 264-, 346-, 410-, and 550-bp regions of E. coli genomic and plasmid DNAs are amplified. The electrophoretic analysis of the products is executed in <3 min following amplification using hydroxyethyl cellulose or poly(dimethylacrylamide) sieving gels. Product sizing is demonstrated by proportioning the amplified product with a DNA sizing ladder.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1021/ac970642d

Cites

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 37

  1. Harrison D. J., 1992, Anal. Chem., 64, 1926, 10.1021/ac00041a030 · DOI · Paper page
  2. Manz A., 1992, J. Chromatogr., 593, 253, 10.1016/0021-9673(92)80293-4 · DOI · Paper page
  3. Seiler K., 1993, Anal. Chem., 65, 1481, 10.1021/ac00058a029 · doi:10.1021/ac00058a029
  4. Harrison D. J., 1993, Science, 261, 895, 10.1126/science.261.5123.895 · DOI · Paper page
  5. Jacobson S. C., 1994, Anal. Chem., 66, 1107, 10.1021/ac00079a028 · DOI · Paper page
  6. Jacobson S. C., 1994, Anal. Chem., 66, 1114, 10.1021/ac00079a029 · DOI · Paper page
  7. Burggraf N., 1993, J. High Resolut. Chromatogr., 16, 594, 10.1002/jhrc.1240161005 · doi:10.1002/jhrc.1240161005
  8. Raymond D. E., 1994, Anal. Chem., 66, 2858, 10.1021/ac00090a011 · doi:10.1021/ac00090a011
  9. Jacobson S. C., 1994, Anal. Chem., 66, 2369, 10.1021/ac00086a024 · doi:10.1021/ac00086a024
  10. Moore A. W., 1995, Anal. Chem., 67, 4184, 10.1021/ac00118a023 · doi:10.1021/ac00118a023
  11. Effenhauser C. S., 1994, Anal. Chem., 66, 2949, 10.1021/ac00090a024 · doi:10.1021/ac00090a024
  12. Woolley A. T., 1994, Proc. Natl. Acad. Sci. U.S.A., 91, 11348, 10.1073/pnas.91.24.11348 · DOI
  13. Woolley A. T., 1995, Anal. Chem., 67, 3676, 10.1021/ac00116a010 · DOI
  14. Volkmuth W. D., 1992, Nature, 358, 600, 10.1038/358600a0 · doi:10.1038/358600a0
  15. Fodor S. P. A., 1991, Science, 251, 767, 10.1126/science.1990438 · DOI · Paper page
  16. Wilding P., 1994, J. Clin. Chem., 40, 1815, 10.1093/clinchem/40.9.1815 · doi:10.1093/clinchem/40.9.1815
  17. Murakami, Y.; Takeuchi, T.; Yokoyama, K.; Tamiya, E.; Karube, I.; Suda, M.Anal. Chem.1993,65, 2731 · doi:10.1021/ac00068a005
  18. Fettinger J. C., 1993, Sens. Actuators B
  19. Hadd A. G., 1997, Anal. Chem., 69, 3407, 10.1021/ac970192p · DOI · Paper page
  20. Jacobson S. C., 1994, Anal. Chem., 66, 4127, 10.1021/ac00095a003 · doi:10.1021/ac00095a003
  21. Jacobson S. C., 1994, Anal. Chem., 66, 3472, 10.1021/ac00092a027 · doi:10.1021/ac00092a027
  22. Fluri K., 1996, J. Anal. Chem., 68, 4285, 10.1021/ac9604090 · doi:10.1021/ac9604090
  23. Jacobson S. C., 1996, Anal. Chem., 68, 720, 10.1021/ac951230c · doi:10.1021/ac951230c
  24. Woolley A. T., 1996, Anal. Chem., 68, 4081, 10.1021/ac960718q · DOI · Paper page
  25. Cheng J., 1996, Mol. Diagn., 1, 183, 10.1016/S1084-8592(96)70004-8 · doi:10.1016/s1084-8592(96)70004-8
  26. Chamberlain S., 1988, Nucleic Acids Res., 16, 11141, 10.1093/nar/16.23.11141 · DOI · Paper page
  27. Erlich H. A., 1992, PCR Technology. Principles and Applications for DNA Amplification
  28. Saika R. K., 1986, Nature, 324, 163, 10.1038/324163a0 · DOI · Paper page
  29. Lee L. G., 1993, Nucleic Acids Res., 21, 3761, 10.1093/nar/21.16.3761 · DOI · Paper page
  30. Bassler H. A., 1995, Appl. Environ. Microbiol., 61, 3724, 10.1128/aem.61.10.3724-3728.1995 · doi:10.1128/aem.61.10.3724-3728.1995
  31. Hjerten S., 1985, J. Chromatogr., 347, 191, 10.1016/S0021-9673(01)95485-8 · DOI · Paper page
  32. Pawlowski K., 1997, Mol. Plant−Microbe Interact., 10, 656, 10.1094/MPMI.1997.10.5.656 · DOI
  33. Bej A., 1991, Appl. Environ. Microbiol., 57, 3529, 10.1128/aem.57.12.3529-3534.1991 · DOI · Paper page
  34. Berka J., 1995, Electrophoresis, 16, 377, 10.1002/elps.1150160164 · doi:10.1002/elps.1150160164
  35. Heiger D. N., 1990, J. Chromatogr., 516, 33, 10.1016/S0021-9673(01)90202-X · DOI · Paper page
  36. Chiari M., 1994, Electrophoresis, 15, 177, 10.1002/elps.1150150131 · doi:10.1002/elps.1150150131
  37. Kilger C., 1997, Biotechniques, 22, 412, 10.2144/97223bm08 · DOI

Cited by

Metadata

Authors
Waters, L. C., Jacobson, S. C., Kroutchinina, N., Khandurina, J., Foote, R. S., & Ramsey, J. M.
Venue
Analytical Chemistry
Publisher
American Chemical Society (ACS)
Published
1998-01-01
Volume
70
Issue
1
Pages
158-162
ISSN
  • 0003-2700 (ISSN-L)
  • 1520-6882
DOI
10.1021/ac970642d
OpenAlex
W2079112687
Cited by
405
References
0
FWCI
35.10
Type
journal article
Language
en
Open access
closed

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.