Effects of personal short-term exposure to ambient ozone on blood pressure and vascular endothelial function: a mechanistic study based on DNA methylation and metabolomics
Abstract
Short-term exposure to ambient ozone is associated with adverse cardiovascular effects, with inconsistent evidence on the molecular mechanisms. We conducted a longitudinal panel study among 43 college students in Shanghai to explore the effects of personal ozone exposure on blood pressure (BP), vascular endothelial function, and the potential molecular mechanisms. We measured real-time personal ozone exposure levels, serum angiotensin-converting enzyme (ACE) and endothelin-1 (ET-1), and locus-specific DNA methylation of ACE and EDN1 (coding ET-1). We used an untargeted metabolomic approach to explore potentially important metabolites. We applied linear mixed-effect models to examine the effects of ozone on the above biomarkers. An increase in 2 h-average ozone exposure was significantly associated with elevated levels of BP, ACE, and ET-1. ACE and EDN1 methylation decreased with ozone exposure, but the magnitude differed by genomic loci. Metabolomics analysis showed significant changes in serum lipid metabolites following ozone exposure that are involved in maintaining vascular endothelial function. Our findings suggested that acute exposure to ambient ozone can elevate serum levels of ACE and ET-1, decrease their DNA methylation, and alter the lipid metabolism, which may be partly responsible for the effects of ozone on BP and vascular endothelial function.
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Short-term exposure to ambient ozone can elevate serum levels of ACE and ET-1, decrease their DNA methylation, and alter the lipid metabolism, which may be partly responsible for increased blood pressure and vascular endothelial disfunction.
“Xia et al. revealed that short-term exposure to ambient ozone can elevate serum levels of ACE and ET-1, decrease their DNA methylation, and alter the lipid metabolism, which may be partly responsible for increased blood pressure and vascular endothelial disfunction (51).”
From Ozone pollution: A Major health hazard worldwide (2019) · cites this paper as [51]
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Ozone pollution: A Major health hazard worldwide
Zhang, J. (., Wei, Y., & Fang, Z. (2019) · Frontiers in Immunology · doi:10.3389/fimmu.2019.02518 · Cited by 828 · see citing works
- Authors
- Xia, Y., Niu, Y., Cai, J., Lin, Z., Liu, C., Li, H., Chen, C., Song, W., Zhao, Z., Chen, R., & Kan, H.
- Affiliations
- Fudan University (CN)
- Corresponding author
- Chen, Renjie
- Kan, Haidong
- ORCID
- Venue
- Environmental Science & Technology
- Publisher
- American Chemical Society (ACS)
- Published
- 2018-09-27
- Volume
- 52
- Issue
- 21
- Pages
- 12774-12782
- ISSN
- 0013-936X (ISSN-L)
- 1520-5851
- OpenAlex
- W2893184798
- Cited by
- 86
- References
- 42
- Citations by year
- 2026: 11 · 2025: 7 · 2024: 10 · 2023: 14 · 2022: 15 · 2021: 14 · 2020: 11 · 2019: 4
- FWCI
- 3.58
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Environmental Science & Technology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- China Medical Board (16-250)
- Science and Technology Commission of Shanghai Municipality (18QA1400400)
- National Natural Science Foundation of China (91643205, 91743111)
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Air Quality and Health Impacts
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Health, Environment, Cognitive Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Keywords
- ambient ozone, short-term air pollution exposure, blood pressure, endothelial function, angiotensin-converting enzyme, endothelin-1, DNA methylation, ACE, serum lipid metabolites, cardiovascular effects of air pollution, university students
- MeSH terms
- Blood Pressure, China, Humans, Ozone, DNA Methylation, Metabolomics
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