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Super-Cationic Carbon Quantum Dots Synthesized from Spermidine as an Eye Drop Formulation for Topical Treatment of Bacterial Keratitis
Abstract
We have developed a one-step method to synthesize carbon quantum dots (CQD PAs ) from biogenic polyamines (PAs) as an antibacterial agent for topical treatment of bacterial keratitis (BK). CQDs synthesized by direct pyrolysis of spermidine (Spd) powder through a simple dry heating treatment exhibit a solubility and yield much higher than those from putrescine and spermine. We demonstrate that CQDs obtained from Spds (CQD Spds ) possess effective antibacterial activities against non-multidrug-resistant Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica serovar Enteritidis bacteria and also against the multidrug-resistant bacteria, methicillin-resistant S. aureus . The minimal inhibitory concentration (MIC) of CQD Spds is ∼2500-fold lower than that of spermidine alone, demonstrating their strong antibacterial capabilities. Investigation of the possible mechanisms behind the antibacterial activities of the as-synthesized CQD Spds indicates that the super-cationic CQD Spds with small size (diameter ca. 6 nm) and highly positive charge (ζ-potential ca. +45 mV) cause severe disruption of the bacterial membrane. In vitro cytotoxicity, hemolysis, hemagglutination, genotoxicity, and oxidative stress and in vivo morphologic and physiologic cornea change evaluations show the good biocompatibility of CQD Spds . Furthermore, topical ocular administration of CQD Spds can induce the opening of the tight junction of corneal epithelial cells, thereby leading to great antibacterial treatment of S. aureus -induced BK in rabbits. Our results suggest that CQD Spds are a promising antibacterial candidate for clinical applications in treating eye-related bacterial infections and even persistent bacteria-induced infections.
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Cites
Carbon quantum dots and their applications
Lim, S. Y., Shen, W., & Gao, Z. (2015) · Chemical Society Reviews · doi:10.1039/c4cs00269e · Cited by 4,976
Review on Zinc Oxide Nanoparticles: Antibacterial Activity and Toxicity Mechanism
Sirelkhatim, A., Mahmud, S., Seeni, A., Kaus, N. H. M., Ann, L. C., Bakhori, S. K. M., Hasan, H., & Mohamad, D. (2015) · Nano-Micro Letters · doi:10.1007/s40820-015-0040-x · Cited by 4,586
Liu, S., Zeng, T. H., Hofmann, M., Burcombe, E., Wei, J., Jiang, R., Kong, J., & Chen, Y. (2011) · ACS Nano · doi:10.1021/nn202451x · Cited by 2,929
Antibacterial properties of nanoparticles
Hajipour, M. J., Fromm, K. M., Akbar Ashkarran, A., Jimenez de Aberasturi, D., Larramendi, I. R. D., Rojo, T., Serpooshan, V., Parak, W. J., & Mahmoudi, M. (2012) · Trends in Biotechnology · doi:10.1016/j.tibtech.2012.06.004 · Cited by 2,710
Glowing Graphene Quantum Dots and Carbon Dots: Properties, Syntheses, and Biological Applications
Zheng, X. T., Ananthanarayanan, A., Luo, K. Q., & Chen, P. (2015) · Small · doi:10.1002/smll.201402648 · Cited by 2,190
Silver nanoparticles: A new view on mechanistic aspects on antimicrobial activity
Durán, N., Durán, M., de Jesus, M. B., Seabra, A. B., Fávaro, W. J., & Nakazato, G. (2016) · Nanomedicine: Nanotechnology, Biology and Medicine · doi:10.1016/j.nano.2015.11.016 · Cited by 1,542
Single-Walled Carbon Nanotubes Exhibit Strong Antimicrobial Activity
Kang, S., Pinault, M., Pfefferle, L. D., & Elimelech, M. (2007) · Langmuir · doi:10.1021/la701067r · Cited by 1,330
Park, M. V. D. Z., Neigh, A. M., Vermeulen, J. P., de la Fonteyne, L. J. J., Verharen, H. W., Briedé, J. J., van Loveren, H., & de Jong, W. H. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.08.085 · Cited by 1,004
Alternative Antimicrobial Approach: Nano-Antimicrobial Materials
Beyth, N., Houri-Haddad, Y., Domb, A., Khan, W., & Hazan, R. (2015) · Evidence-Based Complementary and Alternative Medicine · doi:10.1155/2015/246012 · Cited by 837
Graphene Quantum Dots-Band-Aids Used for Wound Disinfection
Sun, H., Gao, N., Dong, K., Ren, J., & Qu, X. (2014) · ACS Nano · doi:10.1021/nn501640q · Cited by 755
Functions of Polyamines in Mammals
Pegg, A. E. (2016) · Journal of Biological Chemistry · doi:10.1074/jbc.r116.731661 · Cited by 718
Zheng, M., Liu, S., Li, J., Qu, D., Zhao, H., Guan, X., Hu, X., Xie, Z., Jing, X., & Sun, Z. (2014) · Advanced Materials · doi:10.1002/adma.201306192 · Cited by 588
Hühn, D., Kantner, K., Geidel, C., Brandholt, S., De Cock, I., Soenen, S. J. H., Rivera_Gil, P., Montenegro, J.-M., Braeckmans, K., Müllen, K., et al. (2013) · ACS Nano · doi:10.1021/nn3059295 · Cited by 580
Membrane potential is important for bacterial cell division
Strahl, H., & Hamoen, L. W. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1005485107 · Cited by 553
Gan, Z., Xu, H., & Hao, Y. (2016) · Nanoscale · doi:10.1039/c6nr00605a · Cited by 518
Soenen, S. J., Parak, W. J., Rejman, J., & Manshian, B. (2015) · Chemical Reviews · doi:10.1021/cr400714j · Cited by 515
Carbon quantum dot-based nanoprobes for metal ion detection
Gao, X., Du, C., Zhuang, Z., & Chen, W. (2016) · Journal of Materials Chemistry C · doi:10.1039/c6tc02055k · Cited by 505
Jain, A., Duvvuri, L. S., Farah, S., Beyth, N., Domb, A. J., & Khan, W. (2014) · Advanced Healthcare Materials · doi:10.1002/adhm.201400418 · Cited by 485
Keay, L., Edwards, K., Naduvilath, T., Taylor, H. R., Snibson, G. R., Forde, K., & Stapleton, F. (2006) · Ophthalmology · doi:10.1016/j.ophtha.2005.08.013 · Cited by 435
A multifaceted role for polyamines in bacterial pathogens
Shah, P., & Swiatlo, E. (2008) · Molecular Microbiology · doi:10.1111/j.1365-2958.2008.06126.x · Cited by 424
One-step preparation of nitrogen-doped graphenequantum dots from oxidized debris of graphene oxide
Hu, C., Liu, Y., Yang, Y., Cui, J., Huang, Z., Wang, Y., Yang, L., Wang, H., Xiao, Y., & Rong, J. (2013) · J. Mater. Chem. B · doi:10.1039/c2tb00189f · Cited by 421
Carbon Dot-Based Platform for Simultaneous Bacterial Distinguishment and Antibacterial Applications
Yang, J., Zhang, X., Ma, Y.-H., Gao, G., Chen, X., Jia, H.-R., Li, Y.-H., Chen, Z., & Wu, F.-G. (2016) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.6b10398 · Cited by 409
Self-citationDeli, M. A. (2009) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/j.bbamem.2008.09.016 · Cited by 385
Mechanism and consequence of chitosan-mediated reversible epithelial tight junction opening
Yeh, T.-H., Hsu, L.-W., Tseng, M. T., Lee, P.-L., Sonjae, K., Ho, Y.-C., & Sung, H.-W. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.03.056 · Cited by 379
Carbon-based quantum dots for fluorescence imaging of cells and tissues
Luo, P. G., Yang, F., Yang, S.-T., Sonkar, S. K., Yang, L., Broglie, J. J., Liu, Y., & Sun, Y.-P. (2014) · RSC Advances · doi:10.1039/c3ra47683a · Cited by 331
Polyamines in Eukaryotes, Bacteria, and Archaea
Michael, A. J. (2016) · Journal of Biological Chemistry · doi:10.1074/jbc.r116.734780 · Cited by 305
Developments in photocatalytic antibacterial activity of nano TiO2: A review
Yadav, H. M., Kim, J.-S., & Pawar, S. H. (2016) · Korean Journal of Chemical Engineering · doi:10.1007/s11814-016-0118-2 · Cited by 277
Chong, Y., Ge, C., Fang, G., Tian, X., Ma, X., Wen, T., Wamer, W. G., Chen, C., Chai, Z., & Yin, J.-J. (2016) · ACS Nano · doi:10.1021/acsnano.6b04061 · Cited by 272
Characterization of paracellular and aqueous penetration routes in cornea, conjunctiva, and sclera.
Hämäläinen, K. M., Kananen, K., Auriola, S. O. K., Kontturi, K., & Urtti, A. O. (1997) · PubMed · Cited by 266
Visible-Light-Activated Bactericidal Functions of Carbon “Quantum” Dots
Meziani, M. J., Dong, X., Zhu, L., Jones, L. P., LeCroy, G. E., Yang, F., Wang, S., Wang, P., Zhao, Y., Yang, L., et al. (2016) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.6b01765 · Cited by 263
Sonaje, K., Chuang, E.-Y., Lin, K.-J., Yen, T.-C., Su, F.-Y., Tseng, M. T., & Sung, H.-W. (2012) · Molecular Pharmaceutics · doi:10.1021/mp200572t · Cited by 253
Dana, M. R., Qian, Y., & Hamrah, P. (2000) · Cornea · doi:10.1097/00003226-200009000-00008 · Cited by 251
Ananth, A., Dharaneedharan, S., Heo, M.-S., & Mok, Y. S. (2015) · Chemical Engineering Journal · doi:10.1016/j.cej.2014.09.083 · Cited by 248
Pathogenesis of microbial keratitis
Lakhundi, S., Siddiqui, R., & Khan, N. A. (2017) · Microbial Pathogenesis · doi:10.1016/j.micpath.2016.12.013 · Cited by 235
Zeng, Z., Yu, D., He, Z., Liu, J., Xiao, F.-X., Zhang, Y., Wang, R., Bhattacharyya, D., & Tan, T. T. Y. (2016) · Scientific Reports · doi:10.1038/srep20142 · Cited by 226
Hou, J., Wang, W., Zhou, T., Wang, B., Li, H., & Ding, L. (2016) · Nanoscale · doi:10.1039/c6nr02701f · Cited by 218
Insight into the Cellular Internalization and Cytotoxicity of Graphene Quantum Dots
Wu, C., Wang, C., Han, T., Zhou, X., Guo, S., & Zhang, J. (2013) · Advanced Healthcare Materials · doi:10.1002/adhm.201300066 · Cited by 216
Kuo, W.-S., Chen, H.-H., Chen, S.-Y., Chang, C.-Y., Chen, P.-C., Hou, Y.-I., Shao, Y.-T., Kao, H.-F., Lilian Hsu, C.-L., Chen, Y.-C., et al. (2017) · Biomaterials · doi:10.1016/j.biomaterials.2016.12.022 · Cited by 215
Bacteria-derived fluorescent carbon dots for microbial live/dead differentiation
Hua, X.-W., Bao, Y.-W., Wang, H.-Y., Chen, Z., & Wu, F.-G. (2017) · Nanoscale · doi:10.1039/c6nr06558a · Cited by 214
Li, Y., Harroun, S. G., Su, Y., Huang, C., Unnikrishnan, B., Lin, H., Lin, C., & Huang, C. (2016) · Advanced Healthcare Materials · doi:10.1002/adhm.201600297 · Cited by 210
Self-citationZhang, X., Chen, X., Yang, J., Jia, H., Li, Y., Chen, Z., & Wu, F. (2016) · Advanced Functional Materials · doi:10.1002/adfm.201602185 · Cited by 173
Self-citationAntibacterial Property of Graphene Quantum Dots (Both Source Material and Bacterial Shape Matter)
Hui, L., Huang, J., Chen, G., Zhu, Y., & Yang, L. (2016) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.5b10132 · Cited by 171
Hydroxyl radical scavenging and singlet oxygen quenching properties of polyamines
Das, K. C., & Misra, H. P. (2004) · Molecular and Cellular Biochemistry · doi:10.1023/b:mcbi.0000038227.91813.79 · Cited by 166
Üstündağ-Okur, N., Gökçe, E. H., Bozbıyık, D. İ., Eğrilmez, S., Özer, Ö., & Ertan, G. (2014) · European Journal of Pharmaceutical Sciences · doi:10.1016/j.ejps.2014.07.013 · Cited by 163
Polyamine Effects on Antibiotic Susceptibility in Bacteria
Kwon, D.-H., & Lu, C. (2007) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01472-06 · Cited by 158
Shi, J., Sun, X., Lin, Y. M., Zou, X., Li, Z., Liao, Y., Du, M., & Zhang, H. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2014.04.093 · Cited by 154
Aquatic Environmental Risk Assessment for Human Use of the Old Antibiotic Sulfamethoxazole in Europe
Straub, J. O. (2015) · Environmental Toxicology and Chemistry · doi:10.1002/etc.2945 · Cited by 154
The dual roles of functional groups in the photoluminescence of graphene quantum dots
Wang, S., Cole, I. S., Zhao, D., & Li, Q. (2015) · Nanoscale · doi:10.1039/c5nr07042b · Cited by 149
Thomas, P. A., & Geraldine, P. (2007) · Current Opinion in Infectious Diseases · doi:10.1097/qco.0b013e328017f878 · Cited by 131
Carbon Nanomaterials as Antibacterial Colloids
Maas, M. (2016) · Materials · doi:10.3390/ma9080617 · Cited by 119
Polyamines decrease Escherichia coli outer membrane permeability
Vega, A. L. D., & Delcour, A. H. (1996) · Journal of Bacteriology · doi:10.1128/jb.178.13.3715-3721.1996 · Cited by 116
Collagens and proteoglycans of the cornea: importance in transparency and visual disorders
Massoudi, D., Malecaze, F., & Galiacy, S. D. (2015) · Cell and Tissue Research · doi:10.1007/s00441-015-2233-5 · Cited by 112
Lai, J., Hui‐Kang, D., Lai, M.-H., et al. (2013) · PLoS ONE · doi:10.1371/journal.pone.0054058 · Cited by 95
Self-citationMurphy, A., Casey, A., Byrne, G., Chambers, G., & Howe, O. L. (2016) · Journal of Applied Toxicology · doi:10.1002/jat.3315 · Cited by 92
Human corneal cell culture models for drug toxicity studies
Rönkkö, S., Vellonen, K., Järvinen, K., Toropainen, E., & Urtti, A. O. (2016) · Drug Delivery and Translational Research · doi:10.1007/s13346-016-0330-y · Cited by 79
Graphene oxide–cationic polymer conjugates: Synthesis and application as gene delivery vectors
Teimouri, M., Nia, A. H., Abnous, K., et al. (2016) · Plasmid · doi:10.1016/j.plasmid.2016.03.002 · Cited by 54
Topical antibiotic therapy for the treatment of experimental Staphylococcus aureus keratitis.
Callegan, M. C., Hobden, J. A., Hill, J. M., Insler, M. S., & O’Callaghan, R. J. (1992) · PubMed · Cited by 50
Sauer, R., Krug, S. M., Hackel, D., Staat, C., Konasin, N., Yang, S., Niedermirtl, B., Bosten, J., Günther, R., Dabrowski, S., et al. (2014) · Journal of Controlled Release · doi:10.1016/j.jconrel.2014.04.029 · Cited by 43
Role of steroids in the treatment of bacterial keratitis
Henry, C. R., Palioura, S., Amescua, G., & Alfonso, E. C. (2016) · Clinical ophthalmology · doi:10.2147/opth.s80411 · Cited by 36
Effect of Propolis in the Treatment of Experimental Staphylococcus aureus Keratitis in Rabbits
Oksuz, H., Durán, N., Tamer, C., Cetin, M., & Sılıcı, S. (2005) · Ophthalmic Research · doi:10.1159/000087943 · Cited by 35
Pharmacological treatment for infectious corneal ulcers
Miller, D. (2013) · Expert Opinion on Pharmacotherapy · doi:10.1517/14656566.2013.775248 · Cited by 30
Yamaki, T., Kamiya, Y., Ohtake, K., Uchida, M., Seki, T., UEDA, H. M. D., Kobayashi, J., Morimoto, Y., & Natsume, H. (2014) · Pharmaceutical Research · doi:10.1007/s11095-014-1324-4 · Cited by 28
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Novel properties and applications of carbon nanodots
Xiao, L., & Sun, H. (2018) · Nanoscale Horizons · doi:10.1039/c8nh00106e · Cited by 359
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Zhao, Y., Zhang, Z., Pan, Z., & Liu, Y. (2021) · Exploration · doi:10.1002/exp.20210089 · Cited by 357
Nguyen, D. D., & Lai, J.-Y. (2022) · Chemical Engineering Journal · doi:10.1016/j.cej.2022.134970 · Cited by 178
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Metadata
- Authors
- Jian, H.-J., Wu, R.-S., Lin, T.-Y., Li, Y.-J., Lin, H.-J., Harroun, S. G., Lai, J.-Y., & Huang, C.-C.
- Venue
- ACS Nano
- Publisher
- American Chemical Society (ACS)
- Published
- 2017-07-05
- Volume
- 11
- Issue
- 7
- Pages
- 6703-6716
- ISSN
- 1936-0851 (ISSN-L)
- 1936-086X
- OpenAlex
- W2726087978
- Cited by
- 507
- References
- 62
- FWCI
- 11.40
- Type
- journal article
- Language
- en
- Open access
- closed
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