The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Grape-Seed Procyanidins Act as Antiinflammatory Agents in Endotoxin-Stimulated RAW 264.7 Macrophages by Inhibiting NFkB Signaling Pathway
Abstract
Procyanindin extract (PE) is a mixture of polyphenols, mainly procyanidins, obtained from grape seed with putative antiinflammatory activity. We evaluated the PE effect on RAW 264.7 macrophages stimulated with lipopolysaccharide plus interferon-gamma that show a rapid enhanced production of prostaglandin E2 (PGE2) and nitric oxide (NO). Our results demonstrated that PE significantly inhibited the overproduction of NO, dose and time dependently. PE caused a marked inhibition of PGE2 synthesis when administered during activation. Moreover, PE pretreatment diminished iNOS mRNA and protein amount dose dependently (10-65 microg/mL). PE (65 microg/mL) pretreatment inhibited NFkappaB (p65) translocation to nucleus by nearly 40%. Trimeric and longer oligomeric-rich procyanidin fractions from PE (5-30 microg/mL) inhibited iNOS expression but not the monomeric forms catechin and epicatechin. Thus, we show that the degree of polymerization is important in determining procyanidin effects. PE was considerably a more effective inhibitor of NO biosynthesis (IC50 = 50 microg/mL) in comparison to other antiinflammatories, such as aspirin (3 mM), indomethacin (20 microM), and dexamethasone (9 nM). In conclusion, PE modulates inflammatory response in activated macrophages by the inhibition of NO and PGE2 production, suppression of iNOS expression, and NFkB translocation. These results demonstrate an immunomodulatory role of grape seed procyanidins and thus a potential health-benefit in inflammatory conditions that exert an overproduction of NO and PGE2.
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Cites
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Regulation of inflammation and redox signaling by dietary polyphenols
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Cited by
Biological Activities of Polyphenols from Grapes
Xia, E.-Q., Deng, G.-F., Guo, Y.-J., & Li, H.-B. (2010) · International Journal of Molecular Sciences · doi:10.3390/ijms11020622 · Cited by 1,063
Huang, Q., Liu, X., Zhao, G., Hu, T., & Wang, Y. (2018) · Animal nutrition · doi:10.1016/j.aninu.2017.09.004 · Cited by 555
Maier, T., Schieber, A., Kammerer, D. R., & Carle, R. (2009) · Food Chemistry · doi:10.1016/j.foodchem.2008.06.005 · Cited by 379
Terra, X., Montagut, G., Bustos, M., Llopiz, N., Ardèvol, A., Bladé, C., Fernández-Larrea, J., Pujadas, G., Salvadó, J., Arola, L., et al. (2009) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2008.02.005 · Cited by 317
Self-citationDrosou, C., Kyriakopoulou, K., Bimpilas, A., Tsimogiannis, D., & Krokida, M. (2015) · Industrial Crops and Products · doi:10.1016/j.indcrop.2015.05.063 · Cited by 311
Metadata
- Authors
- Terra, X., Valls, J., Vitrac, X., Mérrillon, J.-M., Arola, L., Ardèvol, A., Bladé, C., Fernández-Larrea, J., Pujadas, G., Salvadó, J., & Blay, M.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2007-04-27
- Volume
- 55
- Issue
- 11
- Pages
- 4357-4365
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W1985058212
- Cited by
- 292
- References
- 36
- FWCI
- 10.95
- Type
- journal article
- Language
- en
- Open access
- closed
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- Terra, X., Valls, J., Vitrac, X., Mérrillon, J.-M., Arola, L., Ardèvol, A., Bladé, C., Fernández-Larrea, J., Pujadas, G., Salvadó, J., & Blay, M. (2007). Grape-Seed Procyanidins Act as Antiinflammatory Agents in Endotoxin-Stimulated RAW 264.7 Macrophages by Inhibiting NFkB Signaling Pathway. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf0633185
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