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Cited by 292

The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.

Grape-Seed Procyanidins Act as Antiinflammatory Agents in Endotoxin-Stimulated RAW 264.7 Macrophages by Inhibiting NFkB Signaling Pathway

Terra, Ximena, Valls, Josep, Vitrac, Xavier, Mérrillon, Jean-Michel, Arola, Lluís, Ardèvol, Anna, Bladé, Cinta, Fernández-Larrea, Juan, Pujadas, Gerard, Salvadó, Josepa, Blay, Mayte·2007·Journal of Agricultural and Food Chemistry
DOI 10.1021/jf0633185
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Abstract

Procyanindin extract (PE) is a mixture of polyphenols, mainly procyanidins, obtained from grape seed with putative antiinflammatory activity. We evaluated the PE effect on RAW 264.7 macrophages stimulated with lipopolysaccharide plus interferon-gamma that show a rapid enhanced production of prostaglandin E2 (PGE2) and nitric oxide (NO). Our results demonstrated that PE significantly inhibited the overproduction of NO, dose and time dependently. PE caused a marked inhibition of PGE2 synthesis when administered during activation. Moreover, PE pretreatment diminished iNOS mRNA and protein amount dose dependently (10-65 microg/mL). PE (65 microg/mL) pretreatment inhibited NFkappaB (p65) translocation to nucleus by nearly 40%. Trimeric and longer oligomeric-rich procyanidin fractions from PE (5-30 microg/mL) inhibited iNOS expression but not the monomeric forms catechin and epicatechin. Thus, we show that the degree of polymerization is important in determining procyanidin effects. PE was considerably a more effective inhibitor of NO biosynthesis (IC50 = 50 microg/mL) in comparison to other antiinflammatories, such as aspirin (3 mM), indomethacin (20 microM), and dexamethasone (9 nM). In conclusion, PE modulates inflammatory response in activated macrophages by the inhibition of NO and PGE2 production, suppression of iNOS expression, and NFkB translocation. These results demonstrate an immunomodulatory role of grape seed procyanidins and thus a potential health-benefit in inflammatory conditions that exert an overproduction of NO and PGE2.

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Crossref · 10.1021/jf0633185

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Metadata

Authors
Terra, X., Valls, J., Vitrac, X., Mérrillon, J.-M., Arola, L., Ardèvol, A., Bladé, C., Fernández-Larrea, J., Pujadas, G., Salvadó, J., & Blay, M.
Venue
Journal of Agricultural and Food Chemistry
Publisher
American Chemical Society (ACS)
Published
2007-04-27
Volume
55
Issue
11
Pages
4357-4365
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
DOI
10.1021/jf0633185
OpenAlex
W1985058212
Cited by
292
References
36
FWCI
10.95
Type
journal article
Language
en
Open access
closed

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