In Vitro Antioxidant and Antiproliferative Activities of Selenium-Containing Phycocyanin from Selenium-Enriched Spirulina platensis
Abstract
Both selenium and phycocyanin have been reported to show potent cancer chemopreventive activities. In this study, we investigated the in vitro antioxidant and antiproliferative activities of selenium-containing phycocyanin (Se-PC) purified from selenium-enriched Spirulina platensis. The antioxidant activity of Se-PC was evaluated by using four different free radical scavenging assays, namely, the 2,2'-azinobis-3-ethylbenzothiazolin-6-sulfonic acid (ABTS) assay, 1,1-diphenyl-2-picryhydrazyl (DPPH) assay, superoxide anion scavenging assay, and erythrocyte hemolysis assay. The results indicated that Se-PC exhibited stronger antioxidant activity than phycocyanin by scavenging ABTS, DPPH, superoxide anion, and 2,2'-azobis-(2-amidinopropane)dihydrochloride free radicals. Se-PC also showed dose-dependent protective effects on erythrocytes against H 2O 2-induced oxidative DNA damage as evaluated by the Comet assay. Moreover, Se-PC was identified as a potent antiproliferative agent against human melanoma A375 cells and human breast adenocarcinoma MCF-7 cells. Induction of apoptosis in both A375 and MCF-7 cells by Se-PC was evidenced by accumulation of sub-G1 cell populations, DNA fragmentation, and nuclear condensation. Further investigation on intracellular mechanisms indicated that depletion of mitochondrial membrane potential (DeltaPsi m) was involved in Se-PC-induced cell apoptosis. Our findings suggest that Se-PC is a promising organic Se species with potential applications in cancer chemoprevention.
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Extracted Arthrospira platensis polysaccharides with selenium nanoparticles may be a potential candidate against human cancers as a chemopreventive and chemotherapeutic agent, showing a strong antiproliferative factor on human melanoma and breast adenocarcinoma cells.
“Extracted Arthrospira platensis polysaccharides with selenium nanoparticles may be a potential candidate against human cancers as a chemopreventive and chemotherapeutic agent [128] showing a strong antiproliferative factor on human melanoma and breast adenocarcinoma cells [129].”
From Application of Arthrospira platensis for Medicinal Purposes and the Food Industry: A Review of the Literature (2023) · cites this paper as [129]
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- Authors
- Chen, T., & Wong, Y.-S.
- Affiliations
- Chinese University of Hong Kong (HK)
- Corresponding author
- Wong, Yum-Shing
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2008-06-01
- Volume
- 56
- Issue
- 12
- Pages
- 4352-4358
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2012921958
- Cited by
- 157
- References
- 46
- Citations by year
- 2026: 1 · 2025: 8 · 2024: 6 · 2023: 13 · 2022: 16 · 2021: 17 · 2020: 13 · 2019: 8 · 2018: 12 · 2017: 12 · 2016: 5 · 2015: 8 · 2014: 7 · 2013: 8 · 2012: 5
- FWCI
- 9.01
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Health Sciences › Nursing › Nutrition and Dietetics › Selenium in Biological Systems
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Keywords
- phycocyanin, antioxidant activity, antiproliferative activity, free radical scavenging, oxidative DNA damage, apoptosis, mitochondrial membrane potential, chemoprevention, organic selenium
- MeSH terms
- Adenocarcinoma, Antioxidants, Cell Division, Humans, Melanoma, Phycocyanin, Selenium, Free Radical Scavengers, Apoptosis, Cell Line, Tumor, Spirulina
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