Cited by 157Seaweed-derived Bioactive CompoundsSelenium in Biological SystemsAntioxidant Activity and Oxidative Stress

In Vitro Antioxidant and Antiproliferative Activities of Selenium-Containing Phycocyanin from Selenium-Enriched Spirulina platensis

Chen, Tianfeng, Wong, Yum-Shing·2008·Journal of Agricultural and Food Chemistry

Abstract

Both selenium and phycocyanin have been reported to show potent cancer chemopreventive activities. In this study, we investigated the in vitro antioxidant and antiproliferative activities of selenium-containing phycocyanin (Se-PC) purified from selenium-enriched Spirulina platensis. The antioxidant activity of Se-PC was evaluated by using four different free radical scavenging assays, namely, the 2,2'-azinobis-3-ethylbenzothiazolin-6-sulfonic acid (ABTS) assay, 1,1-diphenyl-2-picryhydrazyl (DPPH) assay, superoxide anion scavenging assay, and erythrocyte hemolysis assay. The results indicated that Se-PC exhibited stronger antioxidant activity than phycocyanin by scavenging ABTS, DPPH, superoxide anion, and 2,2'-azobis-(2-amidinopropane)dihydrochloride free radicals. Se-PC also showed dose-dependent protective effects on erythrocytes against H 2O 2-induced oxidative DNA damage as evaluated by the Comet assay. Moreover, Se-PC was identified as a potent antiproliferative agent against human melanoma A375 cells and human breast adenocarcinoma MCF-7 cells. Induction of apoptosis in both A375 and MCF-7 cells by Se-PC was evidenced by accumulation of sub-G1 cell populations, DNA fragmentation, and nuclear condensation. Further investigation on intracellular mechanisms indicated that depletion of mitochondrial membrane potential (DeltaPsi m) was involved in Se-PC-induced cell apoptosis. Our findings suggest that Se-PC is a promising organic Se species with potential applications in cancer chemoprevention.

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Crossref · 10.1021/jf073399k

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  • Extracted Arthrospira platensis polysaccharides with selenium nanoparticles may be a potential candidate against human cancers as a chemopreventive and chemotherapeutic agent, showing a strong antiproliferative factor on human melanoma and breast adenocarcinoma cells.

    “Extracted Arthrospira platensis polysaccharides with selenium nanoparticles may be a potential candidate against human cancers as a chemopreventive and chemotherapeutic agent [128] showing a strong antiproliferative factor on human melanoma and breast adenocarcinoma cells [129].”

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Authors
Chen, T., & Wong, Y.-S.
Affiliations
Chinese University of Hong Kong (HK)
Corresponding author
Wong, Yum-Shing
Venue
Journal of Agricultural and Food Chemistry
Published
2008-06-01
Volume
56
Issue
12
Pages
4352-4358
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
OpenAlex
W2012921958
Cited by
157
References
46
Citations by year
2026: 1 · 2025: 8 · 2024: 6 · 2023: 13 · 2022: 16 · 2021: 17 · 2020: 13 · 2019: 8 · 2018: 12 · 2017: 12 · 2016: 5 · 2015: 8 · 2014: 7 · 2013: 8 · 2012: 5
FWCI
9.01
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Agricultural and Food Chemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
  • Health Sciences › Nursing › Nutrition and Dietetics › Selenium in Biological Systems
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
Keywords
phycocyanin, antioxidant activity, antiproliferative activity, free radical scavenging, oxidative DNA damage, apoptosis, mitochondrial membrane potential, chemoprevention, organic selenium
MeSH terms
Adenocarcinoma, Antioxidants, Cell Division, Humans, Melanoma, Phycocyanin, Selenium, Free Radical Scavengers, Apoptosis, Cell Line, Tumor, Spirulina

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