Stability of Self-Assembled Polymeric Micelles in Serum
Open accessAbstract
The stability of polymeric nanoparticles in serum is critical to their use in drug delivery where dilution after intravenous injection often results in nanoparticle disassembly and drug unloading; however, few investigate this in biologically relevant media. To gain greater insight into nanoparticle stability in blood, the stability of self-assembled polymeric micelles of poly(d,l-lactide-co-2-methyl-2-carboxytrimethylene carbonate)-g-poly(ethylene glycol), P(LA-co-TMCC)-g-PEG, were tested in both serum and individual serum protein solutions. By encapsulating Förster resonance energy transfer pairs and following their release by fluorescence, these micelles demonstrated excellent thermodynamic and kinetic stability in the presence of serum. Further analyses by fast protein liquid chromatography and dynamic light scattering confirmed these data. Moreover, these micelles are compatible with red blood cells, as shown by a hemolysis assay. The stability and compatibility demonstrated in blood suggest that these micelles may be stable in vivo, which is critical for intravenous drug delivery applications. This comprehensive approach to understanding micelle stability and compatibility is broadly applicable.
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Jiwpanich et al. demonstrated significant differences in the stability of micelles depending on the composition (for instance tween 80 and CTAB vs poloxamers) and also evaluated the role of cross-linking in tuning the rate of exchange.
“With this technique, Jiwpanich et al. [232] demonstrated significant differences in the stability of micelles depending on the composition (for instance tween 80 and CTAB vs poloxamers) and also evaluated the role of the cross-linking in tuning the rate of exchange.”
From Polymeric micelles in drug delivery: An insight of the techniques for their characterization and assessment in biorelevant conditions (2021) · cites this paper as [232]
The Results and Discussion (page unavailable) compare the paper’s slow dye exchange with that reported for highly cross-linked nanogels, citing Jiwpanich et al. The Introduction also describes FRET as a way to study exchange between micelles. Our copy does not report a Tween 80/CTAB-versus-poloxamer comparison or say that Jiwpanich et al. tested how cross-linking tunes the exchange rate. (LLM, conf=0.82)
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- Authors
- Lu, J., Owen, S. C., & Shoichet, M. S.
- Affiliations
- University of Toronto (CA)
- Corresponding author
- Shoichet, Molly S.
- Venue
- Macromolecules
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-07-08
- Volume
- 44
- Issue
- 15
- Pages
- 6002-6008
- ISSN
- 0024-9297 (ISSN-L)
- 1520-5835
- PMC
- PMC3148800
- OpenAlex
- W2043461452
- Cited by
- 299
- References
- 25
- Citations by year
- 2026: 5 · 2025: 17 · 2024: 15 · 2023: 11 · 2022: 26 · 2021: 23 · 2020: 23 · 2019: 27 · 2018: 22 · 2017: 25 · 2016: 15 · 2015: 20 · 2014: 26 · 2013: 27 · 2012: 13
- FWCI
- 9.18
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Macromolecules (publishedVersion, other-oa)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- TSpace (University of Toronto) (submittedVersion)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Physical Sciences › Materials Science › Polymers and Plastics › Dendrimers and Hyperbranched Polymers
- Keywords
- polymeric micelles, polyethylene glycol conjugates, drug delivery, fluorescence resonance energy transfer, hemocompatibility, erythrocyte hemolysis, intravenous injection, nanoparticle disassembly, serum proteins
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