Quantum Dot and Cy5.5 Labeled Nanoparticles to Investigate Lipoprotein Biointeractions via Förster Resonance Energy Transfer
Open accessAbstract
The study of lipoproteins, natural nanoparticles comprised of lipids and apolipoproteins that transport fats throughout the body, is of key importance to better understand, treat, and prevent cardiovascular disease. In the current study, we have developed a lipoprotein-based nanoparticle that consists of a quantum dot (QD) core and Cy5.5 labeled lipidic coating. The methodology allows judicious tuning of the QD/Cy5.5 ratio, which enabled us to optimize Förster resonance energy transfer (FRET) between the QD core and the Cy5.5-labeled coating. This phenomenon allowed us to study lipoprotein-lipoprotein interactions, lipid exchange dynamics, and the influence of apolipoproteins on these processes. Moreover, we were able to study HDL-cell interactions and exploit FRET to visualize HDL association with live macrophage cells.
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Modified HDL has been exploited to probe lipoprotein-lipoprotein interactions.
“Modified HDL has also been exploited to probe lipoprotein–lipoprotein interactions138.”
From Perspectives and opportunities for nanomedicine in the management of atherosclerosis (2011) · cites this paper as [138]
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- Authors
- Skajaa, T., Zhao, Y., van den Heuvel, D. J., Gerritsen, H. C., Cormode, D. P., Koole, R., van Schooneveld, M. M., Post, J. A., Fisher, E. A., Fayad, Z. A., de Mello Donega, C., Meijerink, A., & Mulder, W. J. M.
- Affiliations
- Aarhus University Hospital (DK)
- Condensed Matter and Interfaces
- Molecular Biophysics, Debye Institute
- Translational and Molecular Imaging Institute
- Inorganic Chemistry and Catalysis, Debye Institute, Utrecht University, Sorbonnelaan 16, 3584 CA, The Netherlands
- Utrecht University (NL)
- NYU Langone Health (US)
- Corresponding author
- Mulder, Willem J. M.
- ORCID
- Venue
- Nano Letters
- Publisher
- American Chemical Society (ACS)
- Published
- 2010-11-18
- Volume
- 10
- Issue
- 12
- Pages
- 5131-5138
- ISSN
- 1530-6984 (ISSN-L)
- 1530-6992
- PMC
- PMC3256273
- OpenAlex
- W2085194018
- Cited by
- 76
- References
- 48
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 2 · 2022: 1 · 2021: 3 · 2020: 6 · 2019: 7 · 2018: 6 · 2017: 5 · 2016: 6 · 2015: 8 · 2014: 4 · 2013: 8 · 2012: 10
- FWCI
- 3.76
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nano Letters (publishedVersion)
- PubMed (publishedVersion)
- Pure Amsterdam UMC (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Utrecht University Repository (Utrecht University) (submittedVersion)
- Funders
- National Heart, Lung, and Blood Institute (R01HL078667, R01HL071021)
- National Institute of Biomedical Imaging and Bioengineering (R01EB009638, K99EB012165)
- Topics
- Physical Sciences › Materials Science › Materials Chemistry › Quantum Dots Synthesis And Properties
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- quantum dots, Cy5.5, lipoproteins, fluorescence resonance energy transfer, lipid exchange, apolipoproteins, macrophages, cardiovascular disease
- MeSH terms
- Lipoproteins, Protein Binding, Fluorescence Resonance Energy Transfer, Quantum Dots, Nanoparticles
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