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Self-Assembled DNA Nanopores That Span Lipid Bilayers
Abstract
DNA nanotechnology excels at rationally designing bottom-up structures that can functionally replicate naturally occurring proteins. Here we describe the design and generation of a stable DNA-based nanopore that structurally mimics the amphiphilic nature of protein pores and inserts into bilayers to support a steady transmembrane flow of ions. The pore carries an outer hydrophobic belt comprised of small chemical alkyl groups which mask the negatively charged oligonucleotide backbone. This modification overcomes the otherwise inherent energetic mismatch to the hydrophobic environment of the membrane. By merging the fields of nanopores and DNA nanotechnology, we expect that the small membrane-spanning DNA pore will help open up the design of entirely new molecular devices for a broad range of applications including sensing, electric circuits, catalysis, and research into nanofluidics and controlled transmembrane transport.
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Citations
Cites
Folding DNA to create nanoscale shapes and patterns
Rothemund, P. W. K. (2006) · Nature · doi:10.1038/nature04586 · Cited by 7,354
Ion Channels/Excitable Membranes
(2011) · SpringerReference · doi:10.1007/springerreference_33998 · Cited by 4,034
Characterization of individual polynucleotide molecules using a membrane channel
Kasianowicz, J. J., Brandin, E., Branton, D., & Deamer, D. W. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.24.13770 · Cited by 3,362
The potential and challenges of nanopore sequencing
Branton, D., Deamer, D. W., Marziali, A., Bayley, H., Benner, S. A., Butler, T., Di Ventra, M., Garaj, S., Hibbs, A., Huang, X., et al. (2008) · Nature Biotechnology · doi:10.1038/nbt.1495 · Cited by 2,534
Structure of Staphylococcal α-Hemolysin, a Heptameric Transmembrane Pore
Song, L., Hobaugh, M. R., Shustak, C., Cheley, S., Bayley, H., & Gouaux, J. E. (1996) · Science · doi:10.1126/science.274.5294.1859 · Cited by 2,377
Dekker, C. (2007) · Nature Nanotechnology · doi:10.1038/nnano.2007.27 · Cited by 1,933
Continuous base identification for single-molecule nanopore DNA sequencing
Clarke, J., Wu, H.-C., Jayasinghe, L., Patel, A., Reid, S., & Bayley, H. (2009) · Nature Nanotechnology · doi:10.1038/nnano.2009.12 · Cited by 1,810
Nanopore sensors for nucleic acid analysis
Venkatesan, B. M., & Bashir, R. (2011) · Nature Nanotechnology · doi:10.1038/nnano.2011.129 · Cited by 1,302
Rapid prototyping of 3D DNA-origami shapes with caDNAno
Douglas, S. M., Marblestone, A. H., Teerapittayanon, S., Vazquez, A., Church, G. M., & Shih, W. M. (2009) · Nucleic Acids Research · doi:10.1093/nar/gkp436 · Cited by 1,224
Rapid Chiral Assembly of Rigid DNA Building Blocks for Molecular Nanofabrication
Goodman, R. P., Schaap, I. A. T., Tardin, C. F., Erben, C. M., Berry, R. M., Schmidt, C. F., & Turberfield, A. J. (2005) · Science · doi:10.1126/science.1120367 · Cited by 1,171
Nanopore analytics: sensing of single molecules
Howorka, S., & Siwy, Z. (2009) · Chemical Society Reviews · doi:10.1039/b813796j · Cited by 1,170
Self-citationStochastic sensors inspired by biology
Bayley, H., & Cremer, P. S. (2001) · Nature · doi:10.1038/35093038 · Cited by 1,118
Reading DNA at single-nucleotide resolution with a mutant MspA nanopore and phi29 DNA polymerase
Manrao, E. A., Derrington, I. M., Laszlo, A. H., Langford, K. W., Hopper, M. K., Gillgren, N., Pavlenok, M., Niederweis, M., & Gundlach, J. H. (2012) · Nature Biotechnology · doi:10.1038/nbt.2171 · Cited by 965
A primer to scaffolded DNA origami
Castro, C. E., Kilchherr, F., Kim, D.-N., Shiao, E. L., Wauer, T., Wortmann, P., Bathe, M., & Dietz, H. (2011) · Nature Methods · doi:10.1038/nmeth.1570 · Cited by 925
Sequence-specific detection of individual DNA strands using engineered nanopores
Howorka, S., Cheley, S., & Bayley, H. (2001) · Nature Biotechnology · doi:10.1038/90236 · Cited by 760
Self-citationSynthetic Lipid Membrane Channels Formed by Designed DNA Nanostructures
Langecker, M., Arnaut, V., Martin, T. G., List, J., Renner, S., Mayer, M., Dietz, H., & Simmel, F. C. (2012) · Science · doi:10.1126/science.1225624 · Cited by 758
Electrostatic focusing of unlabelled DNA into nanoscale pores using a salt gradient
Wanunu, M., Morrison, W., Rabin, Y., Grosberg, A. Y., & Meller, A. (2010) · Nature Nanotechnology · doi:10.1038/nnano.2009.379 · Cited by 745
Rapid electronic detection of probe-specific microRNAs using thin nanopore sensors
Wanunu, M., Dadosh, T., Ray, V., Jin, J., McReynolds, L., & Drndić, M. (2010) · Nature Nanotechnology · doi:10.1038/nnano.2010.202 · Cited by 736
Enzyme cascades activated on topologically programmed DNA scaffolds
Wilner, O. I., Weizmann, Y., Gill, R., Lioubashevski, O., Freeman, R., & Willner, I. (2009) · Nature Nanotechnology · doi:10.1038/nnano.2009.50 · Cited by 701
Ortega, A., Amorós, D., & García de la Torre, J. (2011) · Biophysical Journal · doi:10.1016/j.bpj.2011.06.046 · Cited by 661
Controlling protein translocation through nanopores with bio-inspired fluid walls
Yusko, E. C., Johnson, J. M., Majd, S., Prangkio, P., Rollings, R. C., Li, J., Yang, J., & Mayer, M. (2011) · Nature Nanotechnology · doi:10.1038/nnano.2011.12 · Cited by 660
Automated forward and reverse ratcheting of DNA in a nanopore at 5-Å precision
Cherf, G. M., Lieberman, K. R., Rashid, H., Lam, C. E., Karplus, K., & Akeson, M. (2012) · Nature Biotechnology · doi:10.1038/nbt.2147 · Cited by 613
A Bipedal DNA Brownian Motor with Coordinated Legs
Omabegho, T., Sha, R., & Seeman, N. C. (2009) · Science · doi:10.1126/science.1170336 · Cited by 589
Goodman, R. P., Berry, R. M., & Turberfield, A. J. (2004) · Chemical Communications · doi:10.1039/b402293a · Cited by 512
Engineered voltage-responsive nanopores
Siwy, Z. S., & Howorka, S. (2010) · Chem. Soc. Rev. · doi:10.1039/b909105j · Cited by 500
Self-citationNanopore-based detection of circulating microRNAs in lung cancer patients
Wang, Y., Zheng, D., Tan, Q., Wang, M. X., & Gu, L.-Q. (2011) · Nature Nanotechnology · doi:10.1038/nnano.2011.147 · Cited by 497
Stochastic sensing of proteins with receptor-modified solid-state nanopores
Wei, R., Gatterdam, V., Wieneke, R., Tampé, R., & Rant, U. (2012) · Nature Nanotechnology · doi:10.1038/nnano.2012.24 · Cited by 487
Programming DNA Tube Circumferences
Yin, P., Hariadi, R. F., Sahu, S., Choi, H. M. T., Park, S. H., LaBean, T. H., & Reif, J. H. (2008) · Science · doi:10.1126/science.1157312 · Cited by 486
Self-assembled DNA nanostructures for distance-dependent multivalent ligand–protein binding
Rinker, S., Ke, Y., Liu, Y., Chhabra, R., & Yan, H. (2008) · Nature Nanotechnology · doi:10.1038/nnano.2008.164 · Cited by 478
A proton pore in a potassium channel voltage sensor reveals a focused electric field
Starace, D. M., & Bezanilla, F. (2004) · Nature · doi:10.1038/nature02270 · Cited by 398
A DNA-based molecular motor that can navigate a network of tracks
Wickham, S. F. J., Bath, J., Katsuda, Y., Endo, M., Hidaka, K., Sugiyama, H., & Turberfield, A. J. (2012) · Nature Nanotechnology · doi:10.1038/nnano.2011.253 · Cited by 371
Direct observation of stepwise movement of a synthetic molecular transporter
Wickham, S. F. J., Endo, M., Katsuda, Y., Hidaka, K., Bath, J., Sugiyama, H., & Turberfield, A. J. (2011) · Nature Nanotechnology · doi:10.1038/nnano.2010.284 · Cited by 368
Movileanu, L., Howorka, S., Braha, O., & Bayley, H. (2000) · Nature Biotechnology · doi:10.1038/80295 · Cited by 366
Self-citationDNA Origami: The Art of Folding DNA
Saccà, B., & Niemeyer, C. M. (2012) · Angewandte Chemie International Edition · doi:10.1002/anie.201105846 · Cited by 361
Stochastic Sensing of Proteins with Receptor-Modified Solid-State Nanopores
Wei, R., Tampé, R., & Rant, U. (2012) · Biophysical Journal · doi:10.1016/j.bpj.2011.11.2351 · Cited by 354
Scaffolded DNA Origami of a DNA Tetrahedron Molecular Container
Ke, Y., Sharma, J., Liu, M., Jahn, K., Liu, Y., & Yan, H. (2009) · Nano Letters · doi:10.1021/nl901165f · Cited by 343
An autonomous polymerization motor powered by DNA hybridization
Venkataraman, S., Dirks, R. M., Rothemund, P. W. K., Winfree, E., & Pierce, N. A. (2007) · Nature Nanotechnology · doi:10.1038/nnano.2007.225 · Cited by 339
Electric-field-induced wetting and dewetting in single hydrophobic nanopores
Powell, M. R., Cleary, L., Davenport, M., Shea, K. J., & Siwy, Z. S. (2011) · Nature Nanotechnology · doi:10.1038/nnano.2011.189 · Cited by 333
Bell, N. A. W., Engst, C., Ablay, M., Divitini, G., Ducati, C., Liedl, T., & Keyser, U. F. (2011) · Nano Letters · doi:10.1021/nl204098n · Cited by 331
Functional nucleic acid nanostructures and DNA machines
Teller, C., & Willner, I. (2010) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2010.06.001 · Cited by 241
Single-molecule transport across an individual biomimetic nuclear pore complex
Kowalczyk, S. W., Kapinos, L., Blosser, T. R., Magalhães, T., van Nies, P., Lim, R. Y. H., & Dekker, C. (2011) · Nature Nanotechnology · doi:10.1038/nnano.2011.88 · Cited by 238
Operation of a DNA Robot Arm Inserted into a 2D DNA Crystalline Substrate
Ding, B., & Seeman, N. C. (2006) · Science · doi:10.1126/science.1131372 · Cited by 228
Bezrukov, S. M., & Kasianowicz, J. J. (1993) · Physical Review Letters · doi:10.1103/physrevlett.70.2352 · Cited by 195
Functional engineered channels and pores (Review)
Bayley, H., & Jayasinghe, L. (2004) · Molecular Membrane Biology · doi:10.1080/09687680410001716853 · Cited by 194
A de novo peptide hexamer with a mutable channel
Zaccai, N. R., Chi, B., Thomson, A. R., Boyle, A. L., Bartlett, G. J., Bruning, M., Linden, N., Sessions, R. B., Booth, P. J., Brady, R. L., et al. (2011) · Nature Chemical Biology · doi:10.1038/nchembio.692 · Cited by 192
Design and construction of a box-shaped 3D-DNA origami
Kuzuya, A., & Komiyama, M. (2009) · Chemical Communications · doi:10.1039/b907800b · Cited by 187
Examining Noise Sources at the Single-Molecule Level: 1/f Noise of an Open Maltoporin Channel
Bezrukov, S. M., & Winterhalter, M. (2000) · Physical Review Letters · doi:10.1103/physrevlett.85.202 · Cited by 150
Sturdier DNA Nanotubes via Ligation
O’Neill, P., Rothemund, P. W. K., Kumar, A., & Fygenson, D. K. (2006) · Nano Letters · doi:10.1021/nl0603505 · Cited by 137
Synthetic pores with reactive signal amplifiers as artificial tongues
Litvinchuk, S., TANAKA, H. K. M., Miyatake, T., et al. (2007) · Nature Materials · doi:10.1038/nmat1933 · Cited by 129
Structure and water permeability of fully hydrated diphytanoylPC
Tristram‐Nagle, S., Kim, D. J., Akhunzada, N., Kučerka, N., Mathai, J., Katsaras, J., Zeidel, M. L., & Nagle, J. F. (2010) · Chemistry and Physics of Lipids · doi:10.1016/j.chemphyslip.2010.04.011 · Cited by 98
Rationally engineering natural protein assemblies in nanobiotechnology
Howorka, S. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.05.003 · Cited by 86
Self-citationMitchell, N., Schlapak, R., Kastner, M., Armitage, D., Chrzanowski, W., Riener, J., Hinterdorfer, P., Ebner, A., & Howorka, S. (2008) · Angewandte Chemie International Edition · doi:10.1002/anie.200804264 · Cited by 84
Self-citationWang, T., Schiffels, D., Cuesta, S. M., Fygenson, D. K., & Seeman, N. C. (2011) · Journal of the American Chemical Society · doi:10.1021/ja207976q · Cited by 78
Nanoscale DNA Tetrahedra Improve Biomolecular Recognition on Patterned Surfaces
Schlapak, R., Danzberger, J., Armitage, D., Morgan, D. J., Ebner, A., Hinterdorfer, P., Pollheimer, P. D., Gruber, H. J., Schaffler, F., & Howorka, S. (2011) · Small · doi:10.1002/smll.201101576 · Cited by 63
Self-citationShibata, A., Abe, H., Ito, M., Kondo, Y., Shimizu, S., Aikawa, K., & Ito, Y. (2009) · Chemical Communications · doi:10.1039/b912896d · Cited by 38
Theoretical analysis of the atomic force microscopy characterization of columnar thin films
Klapetek, P., & Ohlı́dal, I. (2002) · Ultramicroscopy · doi:10.1016/s0304-3991(02)00159-6 · Cited by 38
Single-Molecule AFM Characterization of Individual Chemically Tagged DNA Tetrahedra
Leitner, M., Mitchell, N., Kastner, M., Schlapak, R., Gruber, H. J., Hinterdorfer, P., Howorka, S., & Ebner, A. (2011) · ACS Nano · doi:10.1021/nn201705p · Cited by 33
Self-citationDNA Nanostructures as Models for Evaluating the Role of Enthalpy and Entropy in Polyvalent Binding
Nangreave, J., Yan, H., & Liu, Y. (2011) · Journal of the American Chemical Society · doi:10.1021/ja1103298 · Cited by 30
Chemical Tags Mediate the Orthogonal Self‐Assembly of DNA Duplexes into Supramolecular Structures
Mitchell, N., Ebner, A., Hinterdorfer, P., Tampé, R., & Howorka, S. (2010) · Small · doi:10.1002/smll.201000790 · Cited by 12
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Meng, H.-M., Liu, H., Kuai, H., Peng, R., Mo, L., & Zhang, X.-B. (2016) · Chemical Society Reviews · doi:10.1039/c5cs00645g · Cited by 608
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Metadata
- Authors
- Burns, J. R., Stulz, E., & Howorka, S.
- Venue
- Nano Letters
- Publisher
- American Chemical Society (ACS)
- Published
- 2013-04-30
- Volume
- 13
- Issue
- 6
- Pages
- 2351-2356
- ISSN
- 1530-6984 (ISSN-L)
- 1530-6992
- OpenAlex
- W1967952840
- Cited by
- 298
- References
- 59
- FWCI
- 12.40
- Type
- journal article
- Language
- en
- Open access
- closed
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