Layer-by-Layer Nanoparticles with a pH-Sheddable Layer for in Vivo Targeting of Tumor Hypoxia
Open accessAbstract
Inspired by the simplicity and versatility of layer-by-layer (LbL) assembly, we applied multilayered polyelectrolyte assemblies on nanoparticles to create viable systemic delivery systems. Focusing on tumor-specific delivery, LbL nanoparticles that exhibit a pH-sensitive outer stealth layer are demonstrated to target and be retained in hypoxic tumor regions. The neutral layers shed in response to acidity to reveal a charged nanoparticle surface that is readily taken up by tumor cells. The first in vivo demonstration of this mechanism of targeting is presented, as well as an initial examination of the mechanism of uptake of the nanoparticles. We further demonstrate that this concept for tumor targeting is potentially valid for a broad range of cancers, with applicability for therapies that target hypoxic tumor tissue.
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Layer-by-layer (LbL) nanoparticles exhibit a pH-sensitive outer layer that targets and is retained in hypoxic tumor areas.
“Layer‐by‐layer (LbL) nanoparticles exhibit a pH‐sensitive outer layer that has been shown to target and be retained in hypoxic tumor areas.”
From Hypoxia signaling in cancer: Implications for therapeutic interventions (2023) · cites this paper as [187]
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- Authors
- Poon, Z., Chang, D., Zhao, X., & Hammond, P. T.
- Affiliations
- Koch Institute for Integrative Cancer Research At MIT (US)
- Corresponding author
- Hammond, Paula T
- Venue
- ACS Nano
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-04-29
- Volume
- 5
- Issue
- 6
- Pages
- 4284-4292
- ISSN
- 1936-0851 (ISSN-L)
- 1936-086X
- PMC
- PMC3125426
- OpenAlex
- W2171845176
- Cited by
- 343
- References
- 53
- Citations by year
- 2026: 7 · 2025: 9 · 2024: 9 · 2023: 12 · 2022: 9 · 2021: 14 · 2020: 20 · 2019: 21 · 2018: 22 · 2017: 28 · 2016: 45 · 2015: 35 · 2014: 37 · 2013: 36 · 2012: 32
- FWCI
- 14.66
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- ACS Nano (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc)
- Funders
- National Cancer Institute (U54CA151884)
- National Institute of Biomedical Imaging and Bioengineering (R01EB008082)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
- Physical Sciences › Materials Science › Polymers and Plastics › Dendrimers and Hyperbranched Polymers
- Keywords
- layer-by-layer assembly, polyelectrolyte multilayers, pH-responsive nanoparticles, tumor hypoxia, tumor targeting, nanoparticle uptake, systemic drug delivery, cancer therapeutics
- MeSH terms
- Animals, Hypoxia, Electrolytes, Female, HeLa Cells, Humans, Hydrogen-Ion Concentration, Mice, Nude, Neoplasm Transplantation, Neoplasms, Particle Size, Polyethylene Glycols, Surface Properties, Tetrazolium Salts, Thiazoles, Nanotechnology, Cell Line, Tumor, Mice, Nanoparticles
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