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Cited by 1,835Neuroscience and Neuropharmacology ResearchIon channel regulation and functionAmino Acid Enzymes and Metabolism

The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.

NMDA-receptor activation increases cytoplasmic calcium concentration in cultured spinal cord neurones

MacDermott, Amy B., Mayer, Mark L., Westbrook, Gary L., Smith, Stephen J., Barker, Jeffery L.·1986·Nature
DOI 10.1038/321519a0
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Abstract

Excitatory amino acids act via receptor subtypes in the mammalian central nervous system (CNS). The receptor selectively activated by N-methyl-D-aspartic acid (NMDA) has been best characterized using voltage-clamp and single-channel recording; the results suggest that NMDA receptors gate channels that are permeable to Na+, K+ and other monovalent cations. Various experiments suggest that Ca2+ flux is also associated with the activation of excitatory amino-acid receptors on vertebrate neurones. Whether Ca2+ enters through voltage-dependent Ca2+ channels or through excitatory amino-acid-activated channels of one or more subtype is unclear. Mg2+ can be used to distinguish NMDA-receptor-activated channels from voltage-dependent Ca2+ channels, because at micromolar concentrations Mg2+ has little effect on voltage-dependent Ca2+ channels while it enters and blocks NMDA receptor channels. Marked differences in the potency of other divalent cations acting as Ca2+ channel blockers compared with their action as NMDA antagonists also distinguish the NMDA channel from voltage-sensitive Ca2+ channels. However, we now directly demonstrate that excitatory amino acids acting at NMDA receptors on spinal cord neurones increase the intracellular Ca2+ activity, measured using the indicator dye arsenazo III, and that this is the result of Ca2+ influx through NMDA receptor channels. Kainic acid (KA), which acts at another subtype of excitatory amino-acid receptor, was much less effective in trigge…

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Research works printed but not linked: 8

  1. Lansman, J. B., Hess, P. & Tsien, R. W. J. gen. Physiol. (in the press).
  2. Cull-Candy, S. G. & Ogden, D. C. Proc. R. Soc. B224, 367–373 (1985).
  3. Evans, R. H. & Watkins, J. C. J. Physiol., Lond. 277, 57P (1977).
  4. Nowak, L. M. & Ascher, P. Soc. Neurosci. Abstr. 10, 23 (1984).
  5. Mayer, M. L. & Westbrook, G. L. Soc. Neurosci. Abstr. 11, 785 (1985).
  6. Ascher, P. & Nowak, L. J. Physiol., Lond. Proc. (in the press).
  7. Nowak, L. M. & Ascher, P. Soc. Neurosci. Abstr. 11, 953 (1985).
  8. Miledi, R. Proc. R. Soc. B209, 447–452 (1980).

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Metadata

Authors
MacDermott, A. B., Mayer, M. L., Westbrook, G. L., Smith, S. J., & Barker, J. L.
Affiliations
  • National Institutes of Health (US)
  • Yale University (US)
ORCID
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
1986-05
Volume
321
Issue
6069
Pages
519-522
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
DOI
10.1038/321519a0
OpenAlex
W2010926499
Cited by
1,835
References
30
Citations by year
2026: 8 · 2025: 11 · 2024: 14 · 2023: 12 · 2022: 13 · 2021: 15 · 2020: 18 · 2019: 22 · 2018: 15 · 2017: 17 · 2016: 19 · 2015: 20 · 2014: 29 · 2013: 23 · 2012: 24
FWCI
40.51
Type
journal article
Language
en
Update notices
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Amino Acid Enzymes and Metabolism
Keywords
NMDA receptor, glutamate receptors, intracellular calcium, calcium influx, spinal cord neurons, kainic acid, voltage-gated calcium channels, magnesium block, arsenazo III, excitatory amino acid antagonists
MeSH terms
Animals, Aspartic Acid, Calcium, Cells, Cultured, Cytoplasm, Embryo, Mammalian, Neurons, Spinal Cord, Receptors, N-Methyl-D-Aspartate, N-Methylaspartate, Receptors, Neurotransmitter, Mice

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