Cited by 759Developmental Biology and Gene RegulationWnt/β-catenin signaling in development and cancerPluripotent Stem Cells Research

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Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer

Bouwmeester, Tewis, Kim, Sung-Hyun, Sasai, Yoshiki, Lu, Bin, Robertis, Eddy M. De·1996·Nature

DOI 10.1038/382595a0

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Abstract

An abundant cDNA enriched in Spemann's organizer, cerberus, was isolated by differential screening. It encodes a secreted protein that is expressed in the anterior endomesoderm. Microinjection of cerberus mRNA into Xenopus embryos induces ectopic heads, and duplicated hearts and livers. The results suggest a role for a molecule expressed in the anterior endoderm in the induction of head structures in the vertebrate embryo.

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Appendix G: In Situ Hybridization: An Improved Whole-Mount Method for Xenopus Embryos
Harland, R. M. (1991) · Methods in cell biology · doi:10.1016/s0091-679x(08)60307-6 · Cited by 2,048
Expression cloning of noggin, a new dorsalizing factor localized to the Spemann organizer in Xenopus embryos
Smith, W. C., & Harland, R. M. (1992) · Cell · doi:10.1016/0092-8674(92)90316-5 · Cited by 1,184
Xenopus chordin: A novel dorsalizing factor activated by organizer-specific homeobox genes
Sasai, Y. (1994) · Cell · doi:10.1016/0092-8674(94)90068-x · Cited by 1,122
Self-citation
Expression of a xenopus homolog of Brachyury (T) is an immediate-early response to mesoderm induction
Smith, J. C., Price, B. M. J., Green, J. B. A., Weigel, D., & Herrmann, B. G. (1991) · Cell · doi:10.1016/0092-8674(91)90573-h · Cited by 971
Retinoic acid causes an anteroposterior transformation in the developing central nervous system
Durston, A. J., Timmermans, J. P., Hage, W. J., Hendriks, H., de Vries, N. J., Heideveld, M., & Nieuwkoop, P. D. (1989) · Nature · doi:10.1038/340140a0 · Cited by 887
Molecular nature of Spemann's organizer: the role of the Xenopus homeobox gene goosecoid
Cho, K. W. Y., Blumberg, B., Steinbeißer, H., & Robertis, E. M. D. (1991) · Cell · doi:10.1016/0092-8674(91)90288-a · Cited by 807
Self-citation
Requirement for LIml in head-organizer function
Shawlot, W., & Behringer, R. R. (1995) · Nature · doi:10.1038/374425a0 · Cited by 805
Neural Induction by the Secreted Polypeptide Noggin
Lamb, T. M., Knecht, A. K., Smith, W. C., Stachel, S. E., Economides, A. N., Stahl, N., Yancopolous, G. D., & Harland, R. M. (1993) · Science · doi:10.1126/science.8235591 · Cited by 785
A common plan for dorsoventral patterning in Bilateria
De Robertis, E. M., & Sasai, Y. (1996) · Nature · doi:10.1038/380037a0 · Cited by 780
Self-citation
Follistatin, an antagonist of activin, is expressed in the Spemann organizer and displays direct neuralizing activity
Hemmati‐Brivanlou, A., Kelly, O., & Melton, D. A. (1994) · Cell · doi:10.1016/0092-8674(94)90320-4 · Cited by 761

Flagged first, then by citations

Show all 44 references

Research works printed but not linked: 6

Spemann, H. & Mangold, H. Wilhelm Roux Arch. EntwMech. Org. 100, 599–638 (1924).
Nieuwkoop, P. D. & Florschütz, P. A. Archs Biol., Paris 61, 113–150 (1950).
Pasteels, J. Archs Biol., Paris 60, 235–250 (1949).
Hausen, P. & Riebesell, M. The Early development of Xenopus laevis (Springer, Berlin, 1991).
Sasai, Y., Lu, B., Piccolo, S. & De Robertis, E. M. EMBO J. (in the press).
Sive, H. L., Hattori, K. & Weintraub, H. Cell 58, 170–180 (1989).

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Andoniadou, C. L., & Martı́nez-Barberá, J. P. (2013) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-013-1269-5 · Cited by 72
“The ability of Cerberus, a secreted inhibitor of nodal, BMP, and WNT molecules to induce an ectopic full axis when overexpressed in Xenopus confirmed the necessity to repress the BMP/TGFβ and WNT pathways for head formation to occur [16, 51, 64, 115].”
Angiopoietin-like 4 Is a Wnt Signaling Antagonist that Promotes LRP6 Turnover
Kirsch, N., Chang, L.-S., Koch, S., Glinka, A., Dolde, C. E., Colozza, G., Benitez, M. D. J., Robertis, E. M. D., & Niehrs, C. (2017) · Developmental Cell · doi:10.1016/j.devcel.2017.09.011 · Cited by 69
“Since ANGPTL4 inhibits Wnt signaling, this expression domain is particularly revealing since the Spemann organizer is the source of multiple Wnt antagonists, e.g., cerberus, dkk1, and sfrp3/frzb (Bouwmeester et al., 1996; Glinka et al., 1998; Leyns et al., 1997).”
BackgroundSelf-citation
A gene regulatory network controlling hhex transcription in the anterior endoderm of the organizer
Rankin, S. A., Kormish, J. D., Kofron, M. J., Jegga, A. G., & Zorn, A. M. (2011) · Developmental Biology · doi:10.1016/j.ydbio.2010.11.037 · Cited by 69
“The chordomesoderm component regulates trunk formation, whereas the hhex-expressing anterior endoderm regulates head and cardiac induction (Bouwmeester et al., 1996; Foley and Mercola, 2005; Jones et al., 1999; Niehrs, 2004).”
Expression of the zinc finger gene fez-like in zebrafish forebrain
Hashimoto, H., Yabe, T., Hirata, T., Shimizu, T., Bae, Y., Yamanaka, Y., Hirano, T., & Hibi, M. (2000) · Mechanisms of Development · doi:10.1016/s0925-4773(00)00418-4 · Cited by 71
Requirement for anti-dorsalizing morphogenetic protein in organizer patterning
Dosch, R., & Niehrs, C. (2000) · Mechanisms of Development · doi:10.1016/s0925-4773(99)00245-2 · Cited by 71
“ADMP and BMP4 both induce vent gene expression and co-injection of BMP4 with all tested BMP inhibitors, including Chordin, Noggin, Follistatin (Hemmati-Brivanlou et al., 1994) and Cerberus ( Bouwmeester et al., 1996 ), prevents vent gene induction.”
Complexity of the Wnt/β‑catenin pathway: Searching for an activation model
Tortelote, G. G., dos Reis, R. R., Mendes, F. A., & Abreu, J. G. (2017) · Cellular Signalling · doi:10.1016/j.cellsig.2017.08.008 · Cited by 71
Hypoblast controls mesoderm generation and axial patterning in the gastrulating rabbit embryo
Idkowiak, J., Weisheit, G., Plitzner, J., & Viebahn, C. (2004) · Development Genes and Evolution · doi:10.1007/s00427-004-0436-y · Cited by 69
“Dickkopf and Cerberus, both originally discovered in the frog, are causally involved in head induction (Bouwmeester et al. 1996; Glinka et al. 1998).”
Background
Early anteroposterior division of the presumptive neurectoderm in Xenopus.
Gamse, J., & Sive, H. (2001) · Mechanisms of Development · doi:10.1016/s0925-4773(01)00358-6 · Cited by 78
“Templates for sense RNA were as follows: p64TXbM (b-globin) (Krieg and Melton, 1984); pCS21 cerberus (Bouwmeester et al., 1996); pCS21 xnoggin (gift from Richard Harland); p64T-X11 (Xwnt-11) (Ku and Melton, 1993); pSP64T-Wnt3a (Wolda et al.”
Influential
Notchless encodes a novel WD40‐repeat‐containing protein that modulates Notch signaling activity
Royet, J., Bouwmeester, T., & Cohen, S. M. (1998) · The EMBO Journal · doi:10.1093/emboj/17.24.7351 · Cited by 69
“Spatial expression was analyzed by whole-mount in situ hybridization essentially as described previously (Bouwmeester et al., 1996), using antisense RNA synthesized from pBS-XNle linearized with XhoI and transcribed with T3 polymerase.”
MethodSelf-citation
Inhibition of BMP Activity by the FGF Signal Promotes Posterior Neural Development in Zebrafish
Koshida, S., Shinya, M., Nikaido, M., Ueno, N., Schulte‐Merker, S., Kuroiwa, A., & Takeda, H. (2002) · Developmental Biology · doi:10.1006/dbio.2002.0581 · Cited by 69
The role of Otx and Otp genes in brain development
Acampora, D., Postiglione, M. P., Avantaggiato, V., Bonito, M. D., & Simeone, A. (2000) · The International Journal of Developmental Biology · doi:10.1387/ijdb.11061431 · Cited by 69
The Xenopus Emx genes identify presumptive dorsal telencephalon and are induced by head organizer signals
Pannese, M., Lupo, G., Kablar, B., Boncinelli, E., Barsacchi, G., & Vignali, R. (1998) · Mechanisms of Development · doi:10.1016/s0925-4773(98)00034-3 · Cited by 69
Early liver differentiation: genetic potentiation and multilevel growth control.
Zaret, K. S. (1998) · Current Opinion in Genetics and Development · doi:10.1016/s0959-437x(98)80006-3 · Cited by 73
The Pitx2 Homeobox Protein Is Required Early for Endoderm Formation and Nodal Signaling
Faucourt, M., Houliston, E., Besnardeau, L., Kimelman, D., & Lepage, T. (2001) · Developmental Biology · doi:10.1006/dbio.2000.9950 · Cited by 68
Neural induction in Xenopus requires inhibition of Wnt-β-catenin signaling
Heeg‐Truesdell, E., & LaBonne, C. (2006) · Developmental Biology · doi:10.1016/j.ydbio.2006.06.015 · Cited by 68
Short-range Wnt5 signaling initiates specification of sea urchin posterior ectoderm
McIntyre, D. C., Seay, N. W., Croce, J. C., & McClay, D. R. (2013) · Development · doi:10.1242/dev.095844 · Cited by 69
“Wnt signaling then further refines neural fates along the anteroposterior axis, distinguishing brain from spinal cord (Bouwmeester et al., 1996).”
Background
Maternal and Zygotic Activity of the Zebrafish ogon Locus Antagonizes BMP Signaling
Miller-Bertoglio, V. E., Carmany-Rampey, A., Fürthauer, M., González, E. M., Thisse, C., Thisse, B., Halpern, M. E., & Solnica‐Krezel, L. (1999) · Developmental Biology · doi:10.1006/dbio.1999.9384 · Cited by 68
Maternal determinants of embryonic cell fate
Heasman, J. (2006) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2005.11.005 · Cited by 67
Translational embryology: Using embryonic principles to generate pancreatic endocrine cells from embryonic stem cells
Spence, J. R., & Wells, J. M. (2007) · Developmental Dynamics · doi:10.1002/dvdy.21366 · Cited by 64
“…Nodal activity is additionally regulated through proteolytic processing, secreted antagonists, and by the presence or absence of receptor complexes (Bouwmeester et al., 1996; Ding et al., 1998; Roebroek et al., 1998; Constam and Robertson, 2000; Perea-Gomez et al., 2002), it is clear that…”
Background
Molecular mechanisms of neural crest induction
Basch, M. L., García‐Castro, M. I., & Bronner-Fraser, M. E. (2004) · Birth Defects Research Part C Embryo Today Reviews · doi:10.1002/bdrc.20015 · Cited by 66
Is Chordin a Long-Range- or Short-Range-Acting Factor? Roles for BMP1-Related Metalloproteases in Chordin and BMP4 Autofeedback Loop Regulation
Blitz, I. L., Shimmi, O., Wünnenberg-Stapleton, K., O’Connor, M. B., & Cho, K. W. Y. (2000) · Developmental Biology · doi:10.1006/dbio.2000.9740 · Cited by 65
Early embryonic specification of vertebrate cranial placodes
Schlosser, G. (2014) · Wiley Interdisciplinary Reviews Developmental Biology · doi:10.1002/wdev.142 · Cited by 64
DRM/GREMLIN (CKTSF1B1) maps to human chromosome 15 and is highly expressed in adult and fetal brain
Topol, L. Z., Modi, W. S., Koochekpour, S., & Blair, D. G. (2000) · Cytogenetic and Genome Research · doi:10.1159/000015568 · Cited by 63
The choice between epidermal and neural fate: a matter of calcium.
Moreau, M., & Leclerc, C. (2004) · The International Journal of Developmental Biology · doi:10.1387/ijdb.15272372 · Cited by 63
“Xnr3, a Xenopus homologue of the mouse nodal gene (Hansen et al., 1997; Smith et al., 1995) and Cerberus, are also able to induce neural tissue (Bouwmeester et al., 1996).”
Background
Induction of cardiomyocytes by GATA4 inXenopusectodermal explants
Latinkic, B., Kotecha, S., & Mohun, T. J. (2003) · Development · doi:10.1242/dev.00599 · Cited by 63
“Injection of Dishevelled (Dsh) ∆PDZ dominant-negative construct, which inhibits both canonical and PCP WNT pathways, does not alter induction of the cardiac marker MLC2 by GATA4 and Cerberus.”
BackgroundInfluential
4 Retinoids and Related Signals in Early Development of the Vertebrate Central Nervous System
Durston, A. J., van der Wees, J., Pijnappel, W. W. M. P., & Godsave, S. F. (1998) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/s0070-2153(08)60366-x · Cited by 63
Stepwise Chemically Induced Cardiomyocyte Specification of Human Embryonic Stem Cells
Gonzalez, R., Lee, J. W., & Schultz, P. G. (2011) · Angewandte Chemie International Edition · doi:10.1002/anie.201103909 · Cited by 62
GATA6 is essential for endoderm formation from human pluripotent stem cells
Fisher, J. B., Pulakanti, K., Rao, S., & Duncan, S. A. (2017) · Biology Open · doi:10.1242/bio.026120 · Cited by 62
“, 2011), Cer1 (Bouwmeester et al., 1996; Wang et al., 2012) and Gata4 (Rojas et al.”
Activin redux: specification of mesodermal pattern inXenopusby graded concentrations of endogenous activin B
Piepenburg, O., Grimmer, D., Williams, P., & Smith, J. C. (2004) · Development · doi:10.1242/dev.01323 · Cited by 61
“We note that inhibition of all mesoderm-inducing Xenopus nodal-related genes, by expression of the C-terminal region of Cerberus (Bouwmeester et al., 1996), causes severe defects in mesoderm formation.”
Signaling specificities of fibroblast growth factor receptors in early Xenopus embryo
Umbhauer, M., Penzo‐Méndez, A., Clavilier, L., Boucaut, J., & Riou, J. (2000) · Journal of Cell Science · doi:10.1242/jcs.113.16.2865 · Cited by 61
XBMPRII, a novel Xenopus type II receptor mediating BMP signaling in embryonic tissues
Frisch, A., & Wright, C. V. E. (1998) · Development · doi:10.1242/dev.125.3.431 · Cited by 60
TGFβ inhibitors: new and unexpected requirements in vertebrate development
Smith, W. C. (1999) · Trends in Genetics · doi:10.1016/s0168-9525(98)01641-2 · Cited by 61
Neural crests are actively precluded from the anterior neural fold by a novel inhibitory mechanism dependent on Dickkopf1 secreted by the prechordal mesoderm
Carmona‐Fontaine, C., Acuña, G., Ellwanger, K., Niehrs, C., & Mayor, R. (2007) · Developmental Biology · doi:10.1016/j.ydbio.2007.07.006 · Cited by 60
Kruppel-like factor 2 cooperates with the ETS family protein ERG to activateFlk1expression during vascular development
Meadows, S. M., Salanga, M. C., & Krieg, P. A. (2009) · Development · doi:10.1242/dev.029538 · Cited by 60

Authors: Bouwmeester, T., Kim, S.-H., Sasai, Y., Lu, B., & Robertis, E. M. D.
Affiliations: Howard Hughes Medical Institute (US)
ORCID: Bouwmeester, Tewis
Kim, Sung-Hyun
Venue: Nature
Publisher: Springer Science and Business Media LLC
Published: 1996-08
Volume: 382
Issue: 6592
Pages: 595-601
ISSN: 0028-0836 (ISSN-L)
1476-4687
DOI: 10.1038/382595a0
OpenAlex: W2081939122
Cited by: 759
References: 44
Citations by year: 2026: 3 · 2025: 9 · 2024: 15 · 2023: 11 · 2022: 10 · 2021: 7 · 2020: 9 · 2019: 6 · 2018: 10 · 2017: 13 · 2016: 14 · 2015: 14 · 2014: 10 · 2013: 21 · 2012: 23
FWCI: 23.03
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Keywords: Cerberus, Spemann's organizer, anterior endoderm, secreted proteins, head induction, Xenopus embryo, gene expression, embryonic induction
MeSH terms: Amino Acid Sequence, Animals, Base Sequence, Blastomeres, Digestive System, Embryonic Induction, Endoderm, Gastrula, Head, Mesoderm, Molecular Sequence Data, Proteins, RNA, Messenger, Tail, Ultraviolet Rays, Xenopus, Cell Polarity, In Situ Hybridization, DNA Primers, DNA, Complementary, Xenopus Proteins, Intercellular Signaling Peptides and Proteins

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Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer

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