Cited by 759Developmental Biology and Gene RegulationWnt/β-catenin signaling in development and cancerPluripotent Stem Cells Research

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Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer

Bouwmeester, Tewis, Kim, Sung-Hyun, Sasai, Yoshiki, Lu, Bin, Robertis, Eddy M. De·1996·Nature

DOI 10.1038/382595a0

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Abstract

An abundant cDNA enriched in Spemann's organizer, cerberus, was isolated by differential screening. It encodes a secreted protein that is expressed in the anterior endomesoderm. Microinjection of cerberus mRNA into Xenopus embryos induces ectopic heads, and duplicated hearts and livers. The results suggest a role for a molecule expressed in the anterior endoderm in the induction of head structures in the vertebrate embryo.

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Background
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A Novel Xenopus Acetyltransferase with a Dynamic Expression in Early Development
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“Based on the expression patterns of SMO - inducing molecules (18, 40, 41) and using the SMO fate maps (42, 43), we microinjected the SMO precursor cells of the 32 - cell stage embryo with fluorescent dextran lineage tracers, labeling the dorsal - animal (D112 in green) and the dorsal - vegetal…”
BackgroundMethod
A Functional Genome-Wide In Vivo Screen Identifies New Regulators of Signalling Pathways during Early Xenopus Embryogenesis
Zhang, S., Li, J., Lea, R., Amaya, E., & Dorey, K. (2013) · PLoS ONE · doi:10.1371/journal.pone.0079469 · Cited by 7
“Cerberus is a secreted protein expressed in the anterior domain of the mesendoderm [30].”
Background
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Differential expression of BMP inhibitors gremlin and noggin in Hydra suggests distinct roles during budding and patterning of tentacles
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G protein-coupled receptors Flop1 and Flop2 inhibit Wnt/β-catenin signaling and are essential for head formation in Xenopus
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Generation of Hepatocyte-Like Cells from Human Pluripotent Stem Cells
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mNanog Possesses Dorsal Mesoderm-Inducing Ability by Modulating Both BMP and Activin/Nodal Signaling in Xenopus Ectodermal Cells
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“Similar inhibition was observed with Xwnt8, mix, mixer, Cerberus (Cer), and Sox17a [23–27] (Fig.”
Result
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Nakajima, Y., Okamoto, H., & Kubo, T. (2009) · Genes to Cells · doi:10.1111/j.1365-2443.2009.01291.x · Cited by 7
“BMP inhibitors, such as noggin (Smith & Harland 1992), follistatin (Hemmati-Brivanlou et al. 1994), chordin (Sasai et al. 1994) and Cerberus (Bouwmeester et al. 1996), block the BMP-Smad signaling pathway and this affects ectodermal fate to direct differentiation from epidermis to neural tissue.”
Background
Uncorking gastrulation: the morphogenetic movement of bottle cells
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“…the expression of organizer marker genes, such as chordin (chd) and cerberus (cer), whereas lower doses of activin induced the mesodermal marker gene,nXbrachyury (Xbra) in animal cap explants from Xenopus laevis (Bouwmeester et al., 1996, Sasai et al., 1994, Smith, 1993, Smith et al., 1991).”
BackgroundInfluential
Peptide growth factors in amphibian embryogenesis: intersection of modern molecular approaches with traditional inductive interaction paradigms
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“Certain growth factor antagonists embody signal integration in one molecule, for example, Cerberus secreted from the head organizer inhibits BMP, Nodal and Wnt signaling to induce dorsoanterior mesendoderm (Bouwmeester et al., 1996; Piccolo et al., 1999) (Fig.”
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Zaraisky, A. G. (2007) · Molecular Biology · doi:10.1134/s002689330702001x · Cited by 6
“In addition to noggin and chordin, the following BMP-binding factors are involved in neural induction: cerberus [40], Cv2 [41], DAN [42], follistatin [43], gremlin [44], two noggin homologs (noggin2 [45] and noggin4 [46]), tsukushi [47], and fugacin/Xnr3 [48, 49].”
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The Molecular Basis of the Gastrula Organizer in Amphibians and Cnidarians
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XTIF2, a Xenopus homologue of the human transcription intermediary factor, is required for a nuclear receptor pathway that also interacts with CBP to suppress Brachyury and XMyoD
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ZSWIM4 regulates embryonic patterning and BMP signaling by promoting nuclear Smad1 degradation
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Authors: Bouwmeester, T., Kim, S.-H., Sasai, Y., Lu, B., & Robertis, E. M. D.
Affiliations: Howard Hughes Medical Institute (US)
ORCID: Bouwmeester, Tewis
Kim, Sung-Hyun
Venue: Nature
Publisher: Springer Science and Business Media LLC
Published: 1996-08
Volume: 382
Issue: 6592
Pages: 595-601
ISSN: 0028-0836 (ISSN-L)
1476-4687
DOI: 10.1038/382595a0
OpenAlex: W2081939122
Cited by: 759
References: 44
Citations by year: 2026: 3 · 2025: 9 · 2024: 15 · 2023: 11 · 2022: 10 · 2021: 7 · 2020: 9 · 2019: 6 · 2018: 10 · 2017: 13 · 2016: 14 · 2015: 14 · 2014: 10 · 2013: 21 · 2012: 23
FWCI: 23.03
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Keywords: Cerberus, Spemann's organizer, anterior endoderm, secreted proteins, head induction, Xenopus embryo, gene expression, embryonic induction
MeSH terms: Amino Acid Sequence, Animals, Base Sequence, Blastomeres, Digestive System, Embryonic Induction, Endoderm, Gastrula, Head, Mesoderm, Molecular Sequence Data, Proteins, RNA, Messenger, Tail, Ultraviolet Rays, Xenopus, Cell Polarity, In Situ Hybridization, DNA Primers, DNA, Complementary, Xenopus Proteins, Intercellular Signaling Peptides and Proteins

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Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer

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