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Endothelial and perivascular cells maintain haematopoietic stem cells

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Ding, Lei, Saunders, Thomas L., Enikolopov, Grigori, Morrison, Sean J.·2012·Nature
DOI 10.1038/nature10783
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Abstract

Several cell types have been proposed to create niches for haematopoietic stem cells (HSCs). However, the expression patterns of HSC maintenance factors have not been systematically studied and no such factor has been conditionally deleted from any candidate niche cell. Thus, the cellular sources of these factors are undetermined. Stem cell factor (SCF; also known as KITL) is a key niche component that maintains HSCs. Here, using Scfgfp knock-in mice, we found that Scf was primarily expressed by perivascular cells throughout the bone marrow. HSC frequency and function were not affected when Scf was conditionally deleted from haematopoietic cells, osteoblasts, nestin-cre- or nestin-creER-expressing cells. However, HSCs were depleted from bone marrow when Scf was deleted from endothelial cells or leptin receptor (Lepr)-expressing perivascular stromal cells. Most HSCs were lost when Scf was deleted from both endothelial and Lepr-expressing perivascular cells. Thus, HSCs reside in a perivascular niche in which multiple cell types express factors that promote HSC maintenance.

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Metadata

Authors
Ding, L., Saunders, T. L., Enikolopov, G., & Morrison, S. J.
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2012-01
Volume
481
Issue
7382
Pages
457-462
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
DOI
10.1038/nature10783
OpenAlex
W2041357218
Cited by
1,954
References
1
FWCI
93.54
Type
journal article
Language
en
Open access
green
Licence
www.springer.com/tdm (tdm)

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CoChat. (2026). Citation check: Endothelial and perivascular cells maintain haematopoietic stem cells. https://app.cochat.ai/papers/10.1038/nature10783
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Ding, L., Saunders, T. L., Enikolopov, G., & Morrison, S. J. (2012). Endothelial and perivascular cells maintain haematopoietic stem cells. Nature. https://doi.org/10.1038/nature10783

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