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In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration
Open accessAbstract
The Wnt target gene Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5) marks actively dividing stem cells in Wnt-driven, self-renewing tissues such as small intestine and colon, stomach and hair follicles. A three-dimensional culture system allows long-term clonal expansion of single Lgr5(+) stem cells into transplantable organoids (budding cysts) that retain many characteristics of the original epithelial architecture. A crucial component of the culture medium is the Wnt agonist RSPO1, the recently discovered ligand of LGR5. Here we show that Lgr5-lacZ is not expressed in healthy adult liver, however, small Lgr5-LacZ(+) cells appear near bile ducts upon damage, coinciding with robust activation of Wnt signalling. As shown by mouse lineage tracing using a new Lgr5-IRES-creERT2 knock-in allele, damage-induced Lgr5(+) cells generate hepatocytes and bile ducts in vivo. Single Lgr5(+) cells from damaged mouse liver can be clonally expanded as organoids in Rspo1-based culture medium over several months. Such clonal organoids can be induced to differentiate in vitro and to generate functional hepatocytes upon transplantation into Fah(-/-) mice. These findings indicate that previous observations concerning Lgr5(+) stem cells in actively self-renewing tissues can also be extended to damage-induced stem cells in a tissue with a low rate of spontaneous proliferation.
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“Liver organoids derived from leucine-rich repeat–containing G protein–coupled receptor 5 (LGR5)–positive liver progenitor cells (LPC) can be stably maintained for months and can differentiate into functionally competent hepatocytes and cholangiocytes [14–16].”
From High-throughput human microfluidic organoid-on-a-chip platform for modeling liver diseases and screening nanotherapeutics. (2026) · cites this paper as [14]
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“The liver organoid has selfrenewal capability, a key proliferative property for its long-term culture and clonal expansion from single cells with sustained stemness (Huch et al., 2013;Hu et al., 2018).”
From Fast formation and maturation enhancement of human liver organoids using a liver-organoid-on-a-chip (2024) · cites this paper as Huch et al., 2013
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“These studies demonstrated that HPCs arise within the liver, engraft the parenchyma14 and may have some differentiation capacity but did not regenerate significant quantities of liver parenchyma11-13.”
From Hepatic progenitor cells of biliary origin with liver repopulation capacity (2015) · cites this paper as [12]
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Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche
Sato, T., Vries, R. G., Snippert, H. J., van de Wetering, M., Barker, N., Stange, D. E., van Es, J. H., Abo, A., Kujala, P., Peters, P. J., et al. (2009) · Nature · doi:10.1038/nature07935 · Cited by 7,204
Self-citationIdentification of stem cells in small intestine and colon by marker gene Lgr5
Barker, N., van Es, J. H., Kuipers, J., Kujala, P., van den Born, M., Cozijnsen, M., Haegebarth, A., Korving, J., Begthel, H., Peters, P. J., et al. (2007) · Nature · doi:10.1038/nature06196 · Cited by 5,687
Self-citationGeneralized lacZ expression with the ROSA26 Cre reporter strain
Soriano, P. (1999) · Nature Genetics · doi:10.1038/5007 · Cited by 5,237
Cell fusion is the principal source of bone-marrow-derived hepatocytes
Wang, X., Willenbring, H., Akkari, Y. M. N., Torimaru, Y., Foster, M., Al-Dhalimy, M., Lagasse, E., Finegold, M. J., Olson, S. B., & Grompe, M. (2003) · Nature · doi:10.1038/nature01531 · Cited by 1,569
Self-citationLgr5+ve Stem Cells Drive Self-Renewal in the Stomach and Build Long-Lived Gastric Units In Vitro
Barker, N., Huch, M., Kujala, P., van de Wetering, M., Snippert, H. J., van Es, J. H., Sato, T., Stange, D. E., Begthel, H., van den Born, M., et al. (2010) · Cell Stem Cell · doi:10.1016/j.stem.2009.11.013 · Cited by 1,547
Self-citationLgr5 homologues associate with Wnt receptors and mediate R-spondin signalling
de Lau, W., Barker, N., Low, T. Y., Koo, B., Li, V. S. W., Teunissen, H., Kujala, P., Haegebarth, A., Peters, P. J., van de Wetering, M., et al. (2011) · Nature · doi:10.1038/nature10337 · Cited by 1,302
Self-citationLustig, B., Jerchow, B., Sachs, M., Weiler, S., Pietsch, T., Karsten, U., van de Wetering, M., Clevers, H., Schlag, P., Birchmeier, W., et al. (2002) · Molecular and Cellular Biology · doi:10.1128/mcb.22.4.1184-1193.2002 · Cited by 1,198
Self-citationLgr5 marks cycling, yet long-lived, hair follicle stem cells
Jaks, V., Barker, N., Kasper, M., van Es, J. H., Snippert, H. J., Clevers, H., & Toftgård, R. (2008) · Nature Genetics · doi:10.1038/ng.239 · Cited by 966
Self-citationCarmon, K. S., Gong, X. W., Lin, Q., Thomas, A., & Liu, Q. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1106083108 · Cited by 894
Furuyama, K., Kawaguchi, Y., Akiyama, H., Horiguchi, M., Kodama, S., Kuhara, T., Hosokawa, S., Elbahrawy, A., Soeda, T., Koizumi, M., et al. (2010) · Nature Genetics · doi:10.1038/ng.722 · Cited by 841
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Dong, R., Zhang, B., & Zhang, X. (2022) · Cell & Bioscience · doi:10.1186/s13578-022-00890-8 · Cited by 33
Advances in Liver Regeneration: Revisiting Hepatic Stem/Progenitor Cells and Their Origin
Sadri, A.-R., Jeschke, M. G., & Amini‐Nik, S. (2015) · Stem Cells International · doi:10.1155/2016/7920897 · Cited by 33
RSPOs facilitated HSC activation and promoted hepatic fibrogenesis
Yin, X., Yi, H., Wang, L., Wu, W., Wu, X., & Yu, L. (2016) · Oncotarget · doi:10.18632/oncotarget.11654 · Cited by 33
Shibata, W., Kinoshita, H., Hikiba, Y., Sato, T. A., Ishii, Y., Sue, S., Sugimori, M., Suzuki, N., Sakitani, K., Ijichi, H., et al. (2018) · Scientific Reports · doi:10.1038/s41598-018-24375-2 · Cited by 33
Self-citationPreclinical Models of Hepatocellular Carcinoma: Current Utility, Limitations, and Challenges
Cigliano, A., Liao, W., Deiana, G. A., Rizzo, D., Chen, X., & Calvisi, D. F. (2024) · Biomedicines · doi:10.3390/biomedicines12071624 · Cited by 32
Senescence-Inflammatory Regulation of Reparative Cellular Reprogramming in Aging and Cancer
Menéndez, J. A., & Alarcón, T. (2017) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2017.00049 · Cited by 33
Li, L., Qian, M., Chen, I.-H., Finkelstein, D., Onar‐Thomas, A., Johnson, M. D., Calabrese, C. R., Bahrami, A., Lopez-Terrada, D. H., Yang, J. J., et al. (2017) · American Journal Of Pathology · doi:10.1016/j.ajpath.2017.11.013 · Cited by 32
You, D., Guo, L., Li, W., Sun, S., Chen, Y., Chai, R., & Li, H. (2018) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2018.00137 · Cited by 32
Fujiwara, H., Tateishi, K., Misumi, K., Hayashi, A., Igarashi, K., Kato, H., Nakatsuka, T., Suzuki, N., Yamamoto, K., Kudo, Y., et al. (2019) · Scientific Reports · doi:10.1038/s41598-019-55211-w · Cited by 32
Current and Future Perspectives of the Use of Organoids in Radiobiology
Nagle, P. W., & Coppes, R. P. (2020) · Cells · doi:10.3390/cells9122649 · Cited by 32
Chen, M. H., Fu, L., Zhang, F., Yang, Y., & Wu, X. (2021) · Life Sciences · doi:10.1016/j.lfs.2021.119748 · Cited by 32
The In Vitro Response of Tissue Stem Cells to Irradiation With Different Linear Energy Transfers
Nagle, P. W., Hosper, N. A., Ploeg, E. M., van Goethem, M., Brandenburg, S., Langendijk, J. A., Chiu, R. K., & Coppes, R. P. (2016) · International Journal of Radiation Oncology*Biology*Physics · doi:10.1016/j.ijrobp.2016.02.020 · Cited by 32
New Perspectives in Liver Transplantation: From Regeneration to Bioengineering
Bizzaro, D., Russo, F. P., & Burra, P. (2019) · Bioengineering · doi:10.3390/bioengineering6030081 · Cited by 32
Organoids as preclinical models of human disease: progress and applications
Chen, B., Du, C., Wang, M., Guo, J., & Liu, X. (2024) · Medical Review · doi:10.1515/mr-2023-0047 · Cited by 31
Repairing organs: lessons from intestine and liver
Gehart, H., & Clevers, H. C. (2015) · Trends in Genetics · doi:10.1016/j.tig.2015.04.005 · Cited by 31
Self-citationTissue regeneration and the epididymal stem cell
Pinel, L., Mandon, M., & Cyr, D. G. (2019) · Andrology · doi:10.1111/andr.12635 · Cited by 31
Cotovio, J. P., & Fernandes, T. G. (2020) · Bioengineering · doi:10.3390/bioengineering7020036 · Cited by 31
Wang, F., Gao, Y., Xue, S.-T., Zhao, L., Jiang, H., Zhang, T., Li, Y., Zhao, C., Wu, F., Siqin, T., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-41593-z · Cited by 31
Canine Models for Copper Homeostasis Disorders
Wu, X., Leegwater, P. A. J., & Fieten, H. (2016) · International Journal of Molecular Sciences · doi:10.3390/ijms17020196 · Cited by 31
Zhang, H., Siegel, C. T., Shuai, L., Lai, J., Zeng, L., Zhang, Y., Lai, X., Bie, P., & Bai, L. (2016) · Scientific Reports · doi:10.1038/srep21783 · Cited by 30
A decade of liver organoids: Advances in disease modeling
Liu, Y., Sheng, J.-Y., Yang, C.-F., Ding, J., & Chan, Y.-S. (2023) · Clinical and Molecular Hepatology · doi:10.3350/cmh.2022.0428 · Cited by 30
Wang, J., Zhan, H., Wang, Y., Zhao, L., Huang, Y., & Wu, R. (2024) · Stem Cell Research & Therapy · doi:10.1186/s13287-024-03989-6 · Cited by 30
Soh, B.-S., Ng, S., Wu, H., Buac, K., Park, J.-H. C., Lian, X. L., Xu, J., Foo, K. S., Felldin, U., He, X., et al. (2016) · Nature Communications · doi:10.1038/ncomms10774 · Cited by 30
Thompson, R. E., Meyers, M. A., Pukazhenthi, B. S., & Hollinshead, F. (2021) · Cryobiology · doi:10.1016/j.cryobiol.2021.11.003 · Cited by 30
Recent advances in tissue stem cells
Fu, X., He, Q., Tao, Y., Wang, M., Wang, W., Wang, Y., Yu, Q. C., Zhang, F., Zhang, X., Chen, Y., et al. (2021) · Science China Life Sciences · doi:10.1007/s11427-021-2007-8 · Cited by 30
Weng, Y., Han, S., Sekyi, M. T., Su, T., Mattis, A. N., & Chang, T. T. (2022) · Stem Cells · doi:10.1093/stmcls/sxac090 · Cited by 30
Oderberg, I. M., & Goessling, W. (2023) · JCI Insight · doi:10.1172/jci.insight.163929 · Cited by 30
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Siervi, S. D., & Turato, C. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24054529 · Cited by 30
Human induced pluripotent stem cell-derived planar neural organoids assembled on synthetic hydrogels
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de Assuncao, T. M., Jalan‐Sakrikar, N., & Huebert, R. C. (2017) · Seminars in Liver Disease · doi:10.1055/s-0036-1597818 · Cited by 30
Genetic tracing of hepatocytes in liver homeostasis, injury, and regeneration
Wang, Y., Huang, X., He, L., Pu, W., Li, Y., Liu, Q., Li, Y., Zhang, L., Yu, W., Zhao, H., et al. (2017) · Journal of Biological Chemistry · doi:10.1074/jbc.m117.782029 · Cited by 30
The Renaissance of Developmental Biology
Johnston, D. S. (2015) · PLoS Biology · doi:10.1371/journal.pbio.1002149 · Cited by 30
Zheng, L., & Duan, S.-L. (2023) · World Journal of Gastroenterology · doi:10.3748/wjg.v29.i16.2380 · Cited by 29
Liver organoids: updates on generation strategies and biomedical applications
Liu, S., Cheng, C., Zhu, L., Zhao, T., Wang, Z., Yi, X., Yan, F., Wang, X., Li, C., Cui, T., et al. (2024) · Stem Cell Research & Therapy · doi:10.1186/s13287-024-03865-3 · Cited by 29
Zhu, Z., Cheng, Y., Liu, X., Ding, W., Liu, J., Ling, Z., & Wu, L. (2025) · Cell Transplantation · doi:10.1177/09636897241303271 · Cited by 31
The role of R-spondins and their receptors in bone metabolism
Shi, G.-X., Mao, W., Zheng, X., & Jiang, L. (2016) · Progress in Biophysics and Molecular Biology · doi:10.1016/j.pbiomolbio.2016.05.012 · Cited by 29
Li, X., Li, M., Chen, J., Dai, H., Wang, L., Xiong, Y., Zhong, Y., & Zhang, L. (2017) · Oncotarget · doi:10.18632/oncotarget.21792 · Cited by 29
RetractedAdvances in generating liver cells from pluripotent stem cells as a tool for modeling liver diseases
Maepa, W., & Ndlovu, H. (2020) · Stem Cells · doi:10.1002/stem.3154 · Cited by 29
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Sakabe, K., Takebe, T., & Asai, A. (2020) · Clinical Liver Disease · doi:10.1002/cld.855 · Cited by 29
Wang, P., Cong, M., Liu, T., Li, Y., Liu, L., Sun, S., Sun, L., Zhu, Z., Ma, H., You, H., et al. (2020) · Journal of Cellular Physiology · doi:10.1002/jcp.29759 · Cited by 29
Self-renewal and differentiation of rat epididymal basal cells using a novel in vitro organoid model
Pinel, L., & Cyr, D. G. (2021) · Biology of Reproduction · doi:10.1093/biolre/ioab113 · Cited by 29
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Yang, H., Wang, Y., Wang, P., Zhang, N., & Wang, P. (2021) · Cancer Biology and Medicine · doi:10.20892/j.issn.2095-3941.2021.0335 · Cited by 29
Current status and prospects of organoid-based regenerative medicine
Choi, W. H., Bae, D. H., & Yoo, J. (2022) · BMB Reports · doi:10.5483/bmbrep.2022-0195 · Cited by 29
Saito, Y., Nakaoka, T., Sakai, K., Muramatsu, T., Toshimitsu, K., Kimura, M., Kanai∥, T., Sato, T., & Saito, H. (2016) · Scientific Reports · doi:10.1038/srep25311 · Cited by 29
Self-citationRoose, H., Cox, B., Boretto, M., Gysemans, C. A., Vennekens, A., & Vankelecom, H. E. J. (2017) · Scientific Reports · doi:10.1038/s41598-017-16796-2 · Cited by 29
Long-Term Correction of Diabetes in Mice by In Vivo Reprogramming of Pancreatic Ducts
Wang, Y., Dorrell, C., Naugler, W. E., Heskett, M. B., Spellman, P. T., Li, B., Galivo, F., Haft, A., Wakefield, L., & Grompe, M. (2018) · Molecular Therapy · doi:10.1016/j.ymthe.2018.02.014 · Cited by 29
Self-citationXia, M., Chen, Y., He, Y., Li, H., & Li, W. (2020) · Stem Cells · doi:10.1002/stem.3175 · Cited by 29
Gyllborg, D., Ahmed, M., Toledo, E. M., Theofilopoulos, S., Yang, S., ffrench-Constant, C., & Arenas, E. (2018) · Stem Cell Reports · doi:10.1016/j.stemcr.2018.07.014 · Cited by 28
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Chen, F., Wang, Z., Yao, H., Liu, Q., Gan, Y., Xu, S., Bao, H., Jin, Y., Hu, Y., Gao, J., et al. (2025) · Trends in biotechnology · doi:10.1016/j.tibtech.2025.04.009 · Cited by 28
The plastic liver: differentiated cells, stem cells, every cell?
Hindley, C. J., Mastrogiovanni, G., & Huch, M. (2014) · Journal of Clinical Investigation · doi:10.1172/jci78372 · Cited by 28
Self-citationZhang, R., Zheng, Y., Li, B., Nie, Y., Ueno, Y., Tsuchida, T., & Taniguchi, H. (2018) · Stem Cell Research & Therapy · doi:10.1186/s13287-017-0747-3 · Cited by 28
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Mellin, R., & Boddey, J. A. (2019) · Trends in Parasitology · doi:10.1016/j.pt.2019.12.003 · Cited by 28
Ogoke, O., Maloy, M. H., & Parashurama, N. (2020) · Biological reviews/Biological reviews of the Cambridge Philosophical Society · doi:10.1111/brv.12650 · Cited by 28
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Funata, M., Nio, Y., Erion, D. M., Thompson, W. L., & Takebe, T. (2020) · Cell Death and Differentiation · doi:10.1038/s41418-020-00661-3 · Cited by 28
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Caiazza, C., Parisi, S., & Caiazzo, M. (2021) · Biology · doi:10.3390/biology10090835 · Cited by 28
Nigro, A. L., Gallo, A., Bulati, M., Vitale, G., Paini, D. S., Pampalone, M., Galvagno, D., Conaldi, P. G., & Miceli, V. (2021) · Frontiers in Medicine · doi:10.3389/fmed.2021.746298 · Cited by 28
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Rigual, M. D. M., Sánchez, P. S., & Djouder, N. (2022) · Trends in cancer · doi:10.1016/j.trecan.2022.10.005 · Cited by 28
Olayanju, A., Jones, L. G., Greco, K. V., Goldring, C. E., & Ansari, T. (2018) · Journal of Applied Toxicology · doi:10.1002/jat.3641 · Cited by 28
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Jun, D. Y., Kim, S. Y., Na, J. C., Lee, H. H., Kim, J., Yoon, Y. E., Hong, S. J., & Han, W. K. (2018) · PLoS ONE · doi:10.1371/journal.pone.0206447 · Cited by 28
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Chen, Q., Coto‐Llerena, M., Suslov, A., Teixeira, R. D., Fofana, I., Nuciforo, S., Hofmann, M., Thimme, R., Hensel, N., Lohmann, V., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-25218-x · Cited by 28
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Wang, J., Wu, X., Zhao, J., Ren, H., & Zhao, Y. (2024) · Advanced Healthcare Materials · doi:10.1002/adhm.202302217 · Cited by 27
Liang, J., Wei, J., Cao, J., Qian, J., Gao, R., Li, X., Wang, D., Gu, Y., Dong, L., Yu, J., et al. (2023) · Genome Biology · doi:10.1186/s13059-023-03084-8 · Cited by 27
Ma, W., Zheng, Y., Yang, G.-Z., Zhang, H., Lu, M., Ma, H., Wu, C., & Lu, H. (2024) · Materials Horizons · doi:10.1039/d4mh00228h · Cited by 27
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Yuan, L., Dawka, S., Kim, Y., Liebert, A., Rost, F., Arnes‐Benito, R., Baenke, F., Götz, C., Tsang, D., Schuhmann, A., et al. (2025) · Nature · doi:10.1038/s41586-025-09884-1 · Cited by 31
Self-citationDayem, A. A., Lee, S., Kim, K., Lim, K. M., Jeon, T.-I., & Cho, S. (2019) · BMB Reports · doi:10.5483/bmbrep.2019.52.5.089 · Cited by 27
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Chusilp, S., Li, B., Lee, D., Lee, C., Vejchapipat, P., & Pierro, A. (2019) · Pediatric Surgery International · doi:10.1007/s00383-019-04581-3 · Cited by 27
Pluripotency inducing Yamanaka factors: role in stemness and chemoresistance of liver cancer
Fatma, H., & Siddique, H. R. (2021) · Expert Review of Anticancer Therapy · doi:10.1080/14737140.2021.1915137 · Cited by 27
Optimized 3D Culture of Hepatic Cells for Liver Organoid Metabolic Assays
Gamboa, C. M., Wang, Y., Xu, H., Kalemba, K. M., Wondisford, F. E., & Sabaawy, H. E. (2021) · Cells · doi:10.3390/cells10123280 · Cited by 27
Synthetic developmental biology: Engineering approaches to guide multicellular organization
Zarkesh, I., Ashtiani, M. K., Shiri, Z., Aran, S., Braun, T. W., & Baharvand, H. (2022) · Stem Cell Reports · doi:10.1016/j.stemcr.2022.02.004 · Cited by 27
Ghorbaninejad, M., Asadzadeh‐Aghdaei, H., Baharvand, H., & Meyfour, A. (2023) · Life Sciences · doi:10.1016/j.lfs.2023.121506 · Cited by 27
Cell transplantation-based regenerative medicine in liver diseases
Cardinale, V., Lanthier, N., Baptista, P. M., Carpino, G., Carnevale, G., Orlando, G., Angelico, R., Manzia, T. M., Schuppan, D., Pinzani, M., et al. (2023) · Stem Cell Reports · doi:10.1016/j.stemcr.2023.06.005 · Cited by 27
Moad, H. E., & Pioszak, A. A. (2013) · Biochemistry · doi:10.1021/bi401090h · Cited by 27
Garnett, M. J., & McDermott, U. (2014) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2013.12.002 · Cited by 27
Zhang, C., Li, Y., Zhang, X., Liu, L., Tong, H., Han, T., Li, W., Jin, X., Yin, N., Song, T., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-11880-z · Cited by 27
LGR4 protects hepatocytes from injury in mouse
Li, Z., Liu, S., Lou, J., Mulholland, M. T., & Zhang, W. (2018) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00056.2018 · Cited by 27
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Hohwieler, M., Müller, M., Frappart, P., & Heller, S. (2019) · Stem Cells International · doi:10.1155/2019/9301382 · Cited by 27
Nguyen, R., Bae, S. D. W., Zhou, G., Read, S. A., Ahlenstiel, G., George, J., & Qiao, L. (2020) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/j.bbcan.2020.188350 · Cited by 27
Xu, X., Cheng, J., Luo, S., Gong, X., Huang, D., Xu, J., Qian, Y., Wan, X., & Zhou, H. J. (2020) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2020.01.159 · Cited by 27
Targeting the E3 Ubiquitin Ligase PJA1 Enhances Tumor-Suppressing TGFβ Signaling
Chen, J., Mitra, A., Li, S., Song, S., Nguyen, B.-N. H., Chen, J., Shin, J., Gough, N. R., Lin, P., Obias, V., et al. (2020) · Cancer Research · doi:10.1158/0008-5472.can-19-3116 · Cited by 27
Rizwan, M., Fokina, A., Kivijärvi, T., Ogawa, M., Kufleitner, M., Laselva, O., Smith, L. J., Bear, C. E., Ogawa, S., Keller, G. M., et al. (2020) · Advanced Functional Materials · doi:10.1002/adfm.202006116 · Cited by 27
Sheahan, B. J., Freeman, A. N., Keeley, T. M., Samuelson, L. C., Roper, J., Hasapis, S., Lee, C., & Dekaney, C. M. (2021) · Cellular and Molecular Gastroenterology and Hepatology · doi:10.1016/j.jcmgh.2021.01.015 · Cited by 27
Vondráček, J., & Machala, M. (2016) · Stem Cells International · doi:10.1155/2016/4326194 · Cited by 27
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Kim, Y., Kang, M., Mamo, M. G., Adisasmita, M., Huch, M., & Choi, D. (2025) · Clinical and Molecular Hepatology · doi:10.3350/cmh.2024.1040 · Cited by 26
Self-citationMultiple LDLR family members act as entry receptors for yellow fever virus
Chong, Z., Hui, S., Qiu, X., Palakurty, S., Sariol, A., Kaszuba, T., Nguyen, M. N., Li, P., Raju, S., Hall, P. D., et al. (2025) · Nature · doi:10.1038/s41586-025-09689-2 · Cited by 26
Jiang, A., Okabe, H., Popovic, B., Preziosi, M., Pradhan‐Sundd, T., Poddar, M., Singh, S., Bell, A. W., England, S. G., Nagarajan, S., et al. (2019) · American Journal Of Pathology · doi:10.1016/j.ajpath.2018.11.010 · Cited by 26
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Sharma, K., Krohne, T. U., & Busskamp, V. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21228484 · Cited by 26
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Ara, T., & Hashimoto, D. (2021) · Frontiers in Immunology · doi:10.3389/fimmu.2021.713631 · Cited by 26
Willemse, J., van der Laan, L. J. W., de Jonge, J., & Verstegen, M. M. A. (2022) · Bioengineering · doi:10.3390/bioengineering9030110 · Cited by 26
Gehling, K., Parekh, S., Schneider, F., Kirchner, M., Kondylis, V., Nikopoulou, C., & Tessarz, P. (2022) · Life Science Alliance · doi:10.26508/lsa.202101340 · Cited by 26
Yu, Y., Zhu, Y.-J., Xiao, Z., Chen, Y., Chang, X., Liu, Y., Tang, Q., & Zhang, H. (2022) · Biomarker Research · doi:10.1186/s40364-022-00421-0 · Cited by 27
Toprakhisar, B., Verfaillie, C. M., & Kumar, M. (2023) · Cells · doi:10.3390/cells12020301 · Cited by 26
Yoon, Y., Gong, S. C., Kim, M. Y., Baik, S. K., Hong, J.-E., Rhee, K., Ryu, H., & Eom, Y. W. (2023) · Cells · doi:10.3390/cells12212514 · Cited by 26
Stopel, A., Lev, C., Dahari, S., Adibi, O., Armon, L., & Gonen, N. (2024) · International Journal of Biological Sciences · doi:10.7150/ijbs.89480 · Cited by 26
Jin, L., Tao, F., Zerda, R., Chen, C., Riggs, A. D., & Ku, H. T. (2013) · Stem Cells and Development · doi:10.1089/scd.2013.0466 · Cited by 26
Malagola, E., Teunissen, M., van der Laan, L. J. W., Verstegen, M. M. A., Schotanus, B. A., van Steenbeek, F. G., Penning, L. C., van Wolferen, M. E., Tryfonidou, M. A., & Spee, B. (2015) · Stem Cells and Development · doi:10.1089/scd.2015.0125 · Cited by 26
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Drost, J., Artegiani, B., & Clevers, H. C. (2016) · Methods in molecular biology · doi:10.1007/978-1-4939-6393-5_15 · Cited by 26
Self-citationFu, X., Lin, J., Qin, F., Yang, Z., Ding, Y., Zhang, Y., Han, L., Zhu, X., & Zhang, Q. (2017) · Molecular Carcinogenesis · doi:10.1002/mc.22764 · Cited by 26
Retracted
Metadata
- Authors
- Huch, M., Dorrell, C., Boj, S. F., van Es, J. H., Li, V. S. W., van de Wetering, M., Sato, T., Hamer, K., Sasaki, N., Finegold, M. J., Haft, A., Vries, R. G., Grompe, M., & Clevers, H.
- Affiliations
- Utrecht University (NL)
- Oregon Health & Science University (US)
- Keio University (JP)
- Baylor College of Medicine (US)
- Corresponding author
- Clevers, Hans
- ORCID
- Venue
- Nature
- Published
- 2013-01-27
- Volume
- 494
- Issue
- 7436
- Pages
- 247-250
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- PMC
- PMC3634804
- OpenAlex
- W2008285918
- Cited by
- 1,555
- References
- 33
- Citations by year
- 2026: 66 · 2025: 117 · 2024: 148 · 2023: 118 · 2022: 122 · 2021: 143 · 2020: 146 · 2019: 120 · 2018: 125 · 2017: 120 · 2016: 109 · 2015: 80 · 2014: 95 · 2013: 43
- FWCI
- 67.87
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- KNAW Research Portal (The Royal Netherlands Academy of Arts and Sciences) (submittedVersion)
- Funders
- Wellcome Trust (104151)
- Japan Society for the Promotion of Science
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK051592, P30DK056338)
- Topics
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- Wnt signaling, liver regeneration, liver organoids, hepatocyte differentiation, bile duct regeneration, tyrosinemia type I, RSPO1, clonal expansion, stem cell transplantation, liver injury, lineage tracing
- MeSH terms
- Alleles, Animals, Bile Ducts, Clone Cells, Culture Media, Disease Models, Animal, Female, Hydrolases, Liver, Liver Diseases, Male, Organoids, Regeneration, Stem Cells, Cell Lineage, Thrombospondins, Tyrosinemias, Hepatocytes, Multipotent Stem Cells, Receptors, G-Protein-Coupled, Mice, Gene Knock-In Techniques, Wnt Signaling Pathway
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