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The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.

Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases

Open access
Hockemeyer, Dirk, Soldner, Frank, Beard, Caroline, Gao, Qing, Mitalipova, Maisam, DeKelver, Russell C, Katibah, George E, Amora, Ranier, Boydston, Elizabeth A, Zeitler, Bryan, Meng, Xiangdong, Miller, Jeffrey C, Zhang, Lei, Rebar, Edward J, Gregory, Philip D, Urnov, Fyodor D, Jaenisch, Rudolf·2009·Nature Biotechnology
DOI 10.1038/nbt.1562
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Abstract

Realizing the full potential of human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) requires efficient methods for genetic modification. However, techniques to generate cell type–specific lineage reporters, as well as reliable tools to disrupt, repair or overexpress genes by gene targeting, are inefficient at best and thus are not routinely used. Here we report the highly efficient targeting of three genes in human pluripotent cells using zinc-finger nuclease (ZFN)–mediated genome editing. First, using ZFNs specific for the OCT4 (POU5F1) locus, we generated OCT4-eGFP reporter cells to monitor the pluripotent state of hESCs. Second, we inserted a transgene into the AAVS1 locus to generate a robust drug-inducible overexpression system in hESCs. Finally, we targeted the PITX3 gene, demonstrating that ZFNs can be used to generate reporter cells by targeting non-expressed genes in hESCs and hiPSCs.

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Research works printed but not linked: 27

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Metadata

Authors
Hockemeyer, D., Soldner, F., Beard, C., Gao, Q., Mitalipova, M., DeKelver, R. C., Katibah, G. E., Amora, R., Boydston, E. A., Zeitler, B., Meng, X., Miller, J. C., Zhang, L., Rebar, E. J., Gregory, P. D., Urnov, F. D., & Jaenisch, R.
Venue
Nature Biotechnology
Publisher
Springer Science and Business Media LLC
Published
2009-08-13
Volume
27
Issue
9
Pages
851-857
ISSN
  • 0733-222X (ISSN-L)
  • 1087-0156
  • 1546-1696
DOI
10.1038/nbt.1562
OpenAlex
W2036632122
Cited by
1,056
References
4
FWCI
34.57
Type
journal article
Language
en
Open access
green
Licence
www.springer.com/tdm (tdm)

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CoChat. (2026). Citation check: Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. https://app.cochat.ai/papers/10.1038/nbt.1562
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Hockemeyer, D., Soldner, F., Beard, C., Gao, Q., Mitalipova, M., DeKelver, R. C., Katibah, G. E., Amora, R., Boydston, E. A., Zeitler, B., Meng, X., Miller, J. C., Zhang, L., Rebar, E. J., Gregory, P. D., Urnov, F. D., & Jaenisch, R. (2009). Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Nature Biotechnology. https://doi.org/10.1038/nbt.1562

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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.